product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-human CD14
catalog :
301828
quantity :
100 tests
price :
264 USD
clonality :
monoclonal
host :
mouse
conjugate :
Pacific Blue
clone name :
M5E2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 27
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1
Risnes L, Christophersen A, Dahal Koirala S, Neumann R, Sandve G, Sarna V, et al. Disease-driving CD4+ T cell clonotypes persist for decades in celiac disease. J Clin Invest. 2018;128:2642-2650 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Dolton G, Rius C, Hasan M, Wall A, Szomolay B, Behiry E, et al. Emergence of immune escape at dominant SARS-CoV-2 killer T cell epitope. Cell. 2022;185:2936-2951.e19 pubmed publisher
Simpson A, Roghanian A, Oldham R, Chan H, Penfold C, Kim H, et al. FcγRIIB controls antibody-mediated target cell depletion by ITIM-independent mechanisms. Cell Rep. 2022;40:111099 pubmed publisher
Schmitz T, Hoffmann V, Olliges E, Bobinger A, Popovici R, Nößner E, et al. Reduced frequency of perforin-positive CD8+ T cells in menstrual effluent of endometriosis patients. J Reprod Immunol. 2021;148:103424 pubmed publisher
Ho C, Silva A, Tomita B, Weng H, Ho I. Differential impacts of TNFα inhibitors on the transcriptome of Th cells. Arthritis Res Ther. 2021;23:199 pubmed publisher
Bernardes J, Mishra N, Tran F, Bahmer T, Best L, Blase J, et al. Longitudinal Multi-omics Analyses Identify Responses of Megakaryocytes, Erythroid Cells, and Plasmablasts as Hallmarks of Severe COVID-19. Immunity. 2020;53:1296-1314.e9 pubmed publisher
Greenshields Watson A, Attaf M, MacLachlan B, Whalley T, Rius C, Wall A, et al. CD4+ T Cells Recognize Conserved Influenza A Epitopes through Shared Patterns of V-Gene Usage and Complementary Biochemical Features. Cell Rep. 2020;32:107885 pubmed publisher
Ilinykh P, Huang K, Santos R, Gilchuk P, Gunn B, Karim M, et al. Non-neutralizing Antibodies from a Marburg Infection Survivor Mediate Protection by Fc-Effector Functions and by Enhancing Efficacy of Other Antibodies. Cell Host Microbe. 2020;27:976-991.e11 pubmed publisher
Kobayashi Y, Yamada D, Kawai T, Sato Y, Teshima T, Yamada Y, et al. Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinoma. Int J Oncol. 2020;56:999-1013 pubmed publisher
van Kempen T, Leijten E, Lindenbergh M, Nordkamp M, Driessen C, Lebbink R, et al. Impaired proteolysis by SPPL2a causes CD74 fragment accumulation that can be recognized by anti-CD74 autoantibodies in human ankylosing spondylitis. Eur J Immunol. 2020;50:1209-1219 pubmed publisher
Han P, Hanlon D, Arshad N, Lee J, Tatsuno K, Robinson E, et al. Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Sci Adv. 2020;6:eaaz1580 pubmed publisher
Longino N, Yang J, Iyer J, Ibrani D, Chow I, Laing K, et al. Human CD4+ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain. Cancer Immunol Res. 2019;7:1727-1739 pubmed publisher
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
Rius C, Attaf M, Tungatt K, Bianchi V, Legut M, Bovay A, et al. Peptide-MHC Class I Tetramers Can Fail To Detect Relevant Functional T Cell Clonotypes and Underestimate Antigen-Reactive T Cell Populations. J Immunol. 2018;200:2263-2279 pubmed publisher
Sander J, Schmidt S, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, et al. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. Immunity. 2017;47:1051-1066.e12 pubmed publisher
Lee J, Tam H, Adler L, Ilstad Minnihan A, Macaubas C, Mellins E. The MHC class II antigen presentation pathway in human monocytes differs by subset and is regulated by cytokines. PLoS ONE. 2017;12:e0183594 pubmed publisher
Crane A, Brubaker W, Johansson J, Trigunaite A, Ceballos J, Bradt B, et al. Peripheral complement interactions with amyloid β peptide in Alzheimer's disease: 2. Relationship to amyloid β immunotherapy. Alzheimers Dement. 2018;14:243-252 pubmed publisher
Hazeldine J, Naumann D, Toman E, Davies D, Bishop J, Su Z, et al. Prehospital immune responses and development of multiple organ dysfunction syndrome following traumatic injury: A prospective cohort study. PLoS Med. 2017;14:e1002338 pubmed publisher
Ciarlo E, Heinonen T, Herderschee J, Fenwick C, Mombelli M, Le Roy D, et al. Impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo. Sci Rep. 2016;6:37944 pubmed publisher
Kerkman P, Kempers A, van der Voort E, van Oosterhout M, Huizinga T, Toes R, et al. Synovial fluid mononuclear cells provide an environment for long-term survival of antibody-secreting cells and promote the spontaneous production of anti-citrullinated protein antibodies. Ann Rheum Dis. 2016;75:2201-2207 pubmed publisher
Holland C, Dolton G, Scurr M, Ladell K, Schauenburg A, Miners K, et al. Enhanced Detection of Antigen-Specific CD4+ T Cells Using Altered Peptide Flanking Residue Peptide-MHC Class II Multimers. J Immunol. 2015;195:5827-36 pubmed publisher
Li H, Evans T, Gillis J, Connole M, Reeves R. Bone marrow-imprinted gut-homing of plasmacytoid dendritic cells (pDCs) in acute simian immunodeficiency virus infection results in massive accumulation of hyperfunctional CD4+ pDCs in the mucosae. J Infect Dis. 2015;211:1717-25 pubmed publisher
Tungatt K, Bianchi V, Crowther M, Powell W, Schauenburg A, Trimby A, et al. Antibody stabilization of peptide-MHC multimers reveals functional T cells bearing extremely low-affinity TCRs. J Immunol. 2015;194:463-74 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
Alkanani A, Rewers M, Dong F, Waugh K, Gottlieb P, Zipris D. Dysregulated Toll-like receptor-induced interleukin-1? and interleukin-6 responses in subjects at risk for the development of type 1 diabetes. Diabetes. 2012;61:2525-33 pubmed
Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301828
Clone :
M5E2
Item :
Pacific Blue™ anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
100 tests
Price (USD) :
264 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PB
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA