product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-human CD14
catalog :
301824
quantity :
100 tests
price :
382 USD
clonality :
monoclonal
host :
mouse
conjugate :
PerCP/Cy5.5
clone name :
M5E2
reactivity :
African green monkey, human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 19
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1c
Kelly A, Günaltay S, McEntee C, Shuttleworth E, Smedley C, Houston S, et al. Human monocytes and macrophages regulate immune tolerance via integrin αvβ8-mediated TGFβ activation. J Exp Med. 2018;215:2725-2736 pubmed publisher
  • flow cytometry; African green monkey; loading ...; fig 2a
Holbrook B, Aycock S, Machiele E, Clemens E, Gries D, Jorgensen M, et al. An R848 adjuvanted influenza vaccine promotes early activation of B cells in the draining lymph nodes of non-human primate neonates. Immunology. 2018;153:357-367 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Lo M, Amarilla A, Lee J, Albornoz E, Modhiran N, Clark R, et al. SARS-CoV-2 triggers complement activation through interactions with heparan sulfate. Clin Transl Immunology. 2022;11:e1413 pubmed publisher
Rodda L, Morawski P, Pruner K, Fahning M, Howard C, Franko N, et al. Imprinted SARS-CoV-2-specific memory lymphocytes define hybrid immunity. Cell. 2022;185:1588-1601.e14 pubmed publisher
Carre C, Wong G, Narang V, Tan C, Chong J, Chin H, et al. Endoplasmic reticulum stress response and bile acid signatures associate with multi-strain seroresponsiveness during elderly influenza vaccination. iScience. 2021;24:102970 pubmed publisher
Cohen C, Li A, Hachim A, Hui D, Kwan M, Tsang O, et al. SARS-CoV-2 specific T cell responses are lower in children and increase with age and time after infection. Nat Commun. 2021;12:4678 pubmed publisher
Savage A, Gutschow M, Chiang T, Henderson K, Green R, Chaudhari M, et al. Multimodal analysis for human ex vivo studies shows extensive molecular changes from delays in blood processing. iScience. 2021;24:102404 pubmed publisher
Marín Franco J, Genoula M, Corral D, Duette G, Ferreyra M, Maio M, et al. Host-Derived Lipids from Tuberculous Pleurisy Impair Macrophage Microbicidal-Associated Metabolic Activity. Cell Rep. 2020;33:108547 pubmed publisher
Rendeiro A, Krausgruber T, Fortelny N, Zhao F, Penz T, Farlik M, et al. Chromatin mapping and single-cell immune profiling define the temporal dynamics of ibrutinib response in CLL. Nat Commun. 2020;11:577 pubmed publisher
Murray L, Xi Y, Upham J. CLEC4C gene expression can be used to quantify circulating plasmacytoid dendritic cells. J Immunol Methods. 2019;464:126-130 pubmed publisher
Liakopoulos V, Jeron A, Shah A, Bruder D, Mertens P, Gorny X. Hemodialysis-related changes in phenotypical features of monocytes. Sci Rep. 2018;8:13964 pubmed publisher
Kubo M, Satoh T, Ishiyama H, Tabata K, Tsumura H, Komori S, et al. Enhanced activated T cell subsets in prostate cancer patients receiving iodine-125 low-dose-rate prostate brachytherapy. Oncol Rep. 2018;39:417-424 pubmed publisher
Corleis B, Lisanti A, Korner C, Schiff A, Rosenberg E, Allen T, et al. Early type I Interferon response induces upregulation of human β-defensin 1 during acute HIV-1 infection. PLoS ONE. 2017;12:e0173161 pubmed publisher
Rusmini M, Griseri P, Lantieri F, Matera I, Hudspeth K, Roberto A, et al. Induction of RET dependent and independent pro-inflammatory programs in human peripheral blood mononuclear cells from Hirschsprung patients. PLoS ONE. 2013;8:e59066 pubmed publisher
Santos J, Barcia R, Simões S, Gaspar M, Calado S, Agua Doce A, et al. The role of human umbilical cord tissue-derived mesenchymal stromal cells (UCX®) in the treatment of inflammatory arthritis. J Transl Med. 2013;11:18 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301824
Clone :
M5E2
Item :
PerCP/Cyanine5.5 anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
100 tests
Price (USD) :
382 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA