product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-human CD14
catalog :
301819
quantity :
25 tests
price :
143 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
M5E2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 42
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2c
Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Shishkova D, Lobov A, Zainullina B, Matveeva V, Markova V, Sinitskaya A, et al. Calciprotein Particles Cause Physiologically Significant Pro-Inflammatory Response in Endothelial Cells and Systemic Circulation. Int J Mol Sci. 2022;23: pubmed publisher
Shemesh A, Su Y, Calabrese D, Chen D, Arakawa Hoyt J, Roybal K, et al. Diminished cell proliferation promotes natural killer cell adaptive-like phenotype by limiting FcεRIγ expression. J Exp Med. 2022;219: pubmed publisher
Mirchandani A, Jenkins S, Bain C, Sánchez García M, Lawson H, Coelho P, et al. Hypoxia shapes the immune landscape in lung injury and promotes the persistence of inflammation. Nat Immunol. 2022;23:927-939 pubmed publisher
Arcangeli S, Bove C, Mezzanotte C, Camisa B, Falcone L, Manfredi F, et al. CAR T cell manufacturing from naive/stem memory T lymphocytes enhances antitumor responses while curtailing cytokine release syndrome. J Clin Invest. 2022;132: pubmed publisher
Paniskaki K, Anft M, Thieme C, Skrzypczyk S, Konik M, Dolff S, et al. Severe Acute Respiratory Syndrome Coronavirus 2 Cross-Reactive B and T Cell Responses in Kidney Transplant Patients. Transplant Proc. 2022;54:1455-1464 pubmed publisher
Liu W. Evaluating Human Natural Killer Cells Antibody-dependent Cellular Cytotoxicity (ADCC) Using Plate-bound Anti-CD16 Antibodies. Bio Protoc. 2022;12:e4285 pubmed publisher
Zhang B, Zhang Y, Xiong L, Li Y, Zhang Y, Zhao J, et al. CD127 imprints functional heterogeneity to diversify monocyte responses in inflammatory diseases. J Exp Med. 2022;219: pubmed publisher
Schneider M, Emmenegger M, Xu C, Condado Morales I, Meisl G, Turelli P, et al. Microfluidic characterisation reveals broad range of SARS-CoV-2 antibody affinity in human plasma. Life Sci Alliance. 2022;5: pubmed publisher
Leboux R, Schipper P, van Capel T, Kong L, van der Maaden K, Kros A, et al. Antigen Uptake After Intradermal Microinjection Depends on Antigen Nature and Formulation, but Not on Injection Depth. Front Allergy. 2021;2:642788 pubmed publisher
Krauss P, Pfeiffenberger M, Damerau A, Buttgereit T, Chen Y, Gaber T, et al. Production of IL-6 and Phagocytosis Are the Most Resilient Immune Functions in Metabolically Compromised Human Monocytes. Front Immunol. 2021;12:730672 pubmed publisher
Pozzetto B, Legros V, Djebali S, Barateau V, Guibert N, Villard M, et al. Immunogenicity and efficacy of          heterologous ChAdOx1-BNT162b2 vaccination. Nature. 2021;600:701-706 pubmed publisher
Schmitz T, Hoffmann V, Olliges E, Bobinger A, Popovici R, Nößner E, et al. Reduced frequency of perforin-positive CD8+ T cells in menstrual effluent of endometriosis patients. J Reprod Immunol. 2021;148:103424 pubmed publisher
Glaros V, Rauschmeier R, Artemov A, Reinhardt A, Ols S, Emmanouilidi A, et al. Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response. Immunity. 2021;54:2005-2023.e10 pubmed publisher
Lee J, Toy L, Kos J, Safonova Y, Schief W, Havenar Daughton C, et al. Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells. NPJ Vaccines. 2021;6:113 pubmed publisher
Zabaleta N, Dai W, Bhatt U, Hérate C, Maisonnasse P, Chichester J, et al. An AAV-based, room-temperature-stable, single-dose COVID-19 vaccine provides durable immunogenicity and protection in non-human primates. Cell Host Microbe. 2021;29:1437-1453.e8 pubmed publisher
Sureshchandra S, Marshall N, Mendoza N, Jankeel A, Zulu M, Messaoudi I. Functional and genomic adaptations of blood monocytes to pregravid obesity during pregnancy. iScience. 2021;24:102690 pubmed publisher
Herati R, Silva L, Vella L, Muselman A, Alanio C, Bengsch B, et al. Vaccine-induced ICOS+CD38+ circulating Tfh are sensitive biosensors of age-related changes in inflammatory pathways. Cell Rep Med. 2021;2:100262 pubmed publisher
Rossignol E, Dugast A, Compere H, Cottrell C, Copps J, Lin S, et al. Mining HIV controllers for broad and functional antibodies to recognize and eliminate HIV-infected cells. Cell Rep. 2021;35:109167 pubmed publisher
Askenase M, Goods B, Beatty H, Steinschneider A, Velazquez S, Osherov A, et al. Longitudinal transcriptomics define the stages of myeloid activation in the living human brain after intracerebral hemorrhage. Sci Immunol. 2021;6: pubmed publisher
Goods B, Askenase M, Markarian E, Beatty H, Drake R, Fleming I, et al. Leukocyte dynamics after intracerebral hemorrhage in a living patient reveal rapid adaptations to tissue milieu. JCI Insight. 2021;6: pubmed publisher
Muto S, Ozaki Y, Yamaguchi H, Mine H, Takagi H, Watanabe M, et al. Tumor β-catenin expression is associated with immune evasion in non-small cell lung cancer with high tumor mutation burden. Oncol Lett. 2021;21:203 pubmed publisher
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
Corrado M, Edwards Hicks J, Villa M, Flachsmann L, Sanin D, Jacobs M, et al. Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity. Cell Metab. 2020;32:981-995.e7 pubmed publisher
Messerer D, Vidoni L, Erber M, Stratmann A, Bauer J, Braun C, et al. Animal-Free Human Whole Blood Sepsis Model to Study Changes in Innate Immunity. Front Immunol. 2020;11:571992 pubmed publisher
Meckiff B, Ramírez Suástegui C, Fajardo V, Chee S, Kusnadi A, Simon H, et al. Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4+ T Cells in COVID-19. Cell. 2020;183:1340-1353.e16 pubmed publisher
Gajdasik D, Gaspal F, Halford E, Fiancette R, Dutton E, Willis C, et al. Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues. Nat Commun. 2020;11:3421 pubmed publisher
Lysenko V, Wildner Verhey van Wijk N, Zimmermann K, Weller M, Bühler M, Wildschut M, et al. Enhanced engraftment of human myelofibrosis stem and progenitor cells in MISTRG mice. Blood Adv. 2020;4:2477-2488 pubmed publisher
Rönnberg E, Ghaib A, Ceriol C, Enoksson M, Arock M, Säfholm J, et al. Divergent Effects of Acute and Prolonged Interleukin 33 Exposure on Mast Cell IgE-Mediated Functions. Front Immunol. 2019;10:1361 pubmed publisher
Cirelli K, Carnathan D, Nogal B, Martin J, Rodriguez O, Upadhyay A, et al. Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance. Cell. 2019;177:1153-1171.e28 pubmed publisher
Janela B, Patel A, Lau M, Goh C, Msallam R, Kong W, et al. A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils. Immunity. 2019;50:1069-1083.e8 pubmed publisher
Kong X, Martinez Barricarte R, Kennedy J, Mele F, Lazarov T, Deenick E, et al. Disruption of an antimycobacterial circuit between dendritic and helper T cells in human SPPL2a deficiency. Nat Immunol. 2018;19:973-985 pubmed publisher
Del Prete G, Oswald K, Lara A, Shoemaker R, Smedley J, Macallister R, et al. Elevated Plasma Viral Loads in Romidepsin-Treated Simian Immunodeficiency Virus-Infected Rhesus Macaques on Suppressive Combination Antiretroviral Therapy. Antimicrob Agents Chemother. 2015;60:1560-72 pubmed publisher
Inglis H, Norris P, Danesh A. Techniques for the analysis of extracellular vesicles using flow cytometry. J Vis Exp. 2015;: pubmed publisher
Kuehn C, Fülöp T, Lakey J, Vermette P. Young porcine endocrine pancreatic islets cultured in fibrin and alginate gels show improved resistance towards human monocytes. Pathol Biol (Paris). 2014;62:354-64 pubmed publisher
Del Prete G, Shoemaker R, Oswald K, Lara A, Trubey C, Fast R, et al. Effect of suberoylanilide hydroxamic acid (SAHA) administration on the residual virus pool in a model of combination antiretroviral therapy-mediated suppression in SIVmac239-infected indian rhesus macaques. Antimicrob Agents Chemother. 2014;58:6790-806 pubmed publisher
Gleason M, Ross J, Warlick E, Lund T, Verneris M, Wiernik A, et al. CD16xCD33 bispecific killer cell engager (BiKE) activates NK cells against primary MDS and MDSC CD33+ targets. Blood. 2014;123:3016-26 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
Del Prete G, Kearney M, Spindler J, Wiegand A, Chertova E, Roser J, et al. Restricted replication of xenotropic murine leukemia virus-related virus in pigtailed macaques. J Virol. 2012;86:3152-66 pubmed publisher
Santer D, Hall B, George T, Tangsombatvisit S, Liu C, Arkwright P, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010;185:4738-49 pubmed publisher
Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301819
Clone :
M5E2
Item :
APC/Cyanine7 anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
25 tests
Price (USD) :
143 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA