LEAF™ Purified anti-human CD14 | BioLegend 301809 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD14

catalog :

301809

quantity :

50 ug

price :

60 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

M5E2

reactivity :

African green monkey, human, dogs, bovine, chimpanzee, rhesus macaque

application :

neutralization, flow cytometry, immunohistochemistry - frozen section

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3a	Allden S, Ogger P, Ghai P, McErlean P, Hewitt R, Toshner R, et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2019;: pubmed publisher
flow cytometry; human; loading ...; fig s1b	Zhang J, Supakorndej T, Krambs J, Rao M, Abou Ezzi G, Ye R, et al. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest. 2019;129:2920-2931 pubmed publisher
flow cytometry; human; loading ...; fig 5	Oda H, Beck D, Kuehn H, Sampaio Moura N, Hoffmann P, Ibarra M, et al. Second Case of HOIP Deficiency Expands Clinical Features and Defines Inflammatory Transcriptome Regulated by LUBAC. Front Immunol. 2019;10:479 pubmed publisher
flow cytometry; rhesus macaque; loading ...; fig 2d	Chea L, Wyatt L, Gangadhara S, Moss B, Amara R. Novel Modified Vaccinia Virus Ankara Vector Expressing Anti-apoptotic Gene B13R Delays Apoptosis and Enhances Humoral Responses. J Virol. 2019;93: pubmed publisher
flow cytometry; human; 1:200; loading ...; fig 1b	Jones G, Bain C, Fenton T, Kelly A, Brown S, Ivens A, et al. Dynamics of Colon Monocyte and Macrophage Activation During Colitis. Front Immunol. 2018;9:2764 pubmed publisher
flow cytometry; human; loading ...; fig s1	Risnes L, Christophersen A, Dahal Koirala S, Neumann R, Sandve G, Sarna V, et al. Disease-driving CD4+ T cell clonotypes persist for decades in celiac disease. J Clin Invest. 2018;128:2642-2650 pubmed publisher
flow cytometry; human; loading ...; fig 2c	Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
flow cytometry; human; loading ...; fig s1	Melo Gonzalez F, Fenton T, Forss C, Smedley C, Goenka A, MacDonald A, et al. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner. J Biol Chem. 2018;293:8543-8553 pubmed publisher
flow cytometry; African green monkey; loading ...; fig 2a	Holbrook B, Aycock S, Machiele E, Clemens E, Gries D, Jorgensen M, et al. An R848 adjuvanted influenza vaccine promotes early activation of B cells in the draining lymph nodes of non-human primate neonates. Immunology. 2018;153:357-367 pubmed publisher
flow cytometry; human; loading ...; fig s4a	Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
flow cytometry; human; fig 2	Offersen R, Nissen S, Rasmussen T, Ã˜stergaard L, Denton P, SÃ¸gaard O, et al. A Novel Toll-Like Receptor 9 Agonist, MGN1703, Enhances HIV-1 Transcription and NK Cell-Mediated Inhibition of HIV-1-Infected Autologous CD4+ T Cells. J Virol. 2016;90:4441-4453 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed

product information

Antigen :

CD14

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Block, IHC-F

Cat # :

301809

Clone :

M5E2

Item :

LEAF™ Purified anti-human CD14

Isotype :

Mouse IgG2a, kappa

Other Names :

LPS receptor

Size :

50 ug

Price (USD) :

60 USD

Reactivity :

Human, African Green, Capuchin Monkey, Cattle (Bovine, Cow), Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Cynomolgus, Dog (Canine), Rhesus, Pigtailed Macaque, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Immunogen :

Full-length human CD14 protein

Application Notes :

. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation 4 , and immunofluorescence microscopy 5 . Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 301846).