product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human CD14
catalog :
301807
quantity :
25 tests
price :
65 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
M5E2
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 45
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 6b
Liu Y, Wang L, Song Q, Ali M, Crowe W, Kucera G, et al. Intrapleural nano-immunotherapy promotes innate and adaptive immune responses to enhance anti-PD-L1 therapy for malignant pleural effusion. Nat Nanotechnol. 2022;17:206-216 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Chen Y, Feng R, He B, Wang J, Xian N, Huang G, et al. PD-1H Expression Associated With CD68 Macrophage Marker Confers an Immune-Activated Microenvironment and Favorable Overall Survival in Human Esophageal Squamous Cell Carcinoma. Front Mol Biosci. 2021;8:777370 pubmed publisher
  • flow cytometry; human; loading ...; fig 7a
Yan D, Wang J, Sun H, Zamani A, Zhang H, Chen W, et al. TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; human; fig 2
Offersen R, Nissen S, Rasmussen T, Østergaard L, Denton P, Søgaard O, et al. A Novel Toll-Like Receptor 9 Agonist, MGN1703, Enhances HIV-1 Transcription and NK Cell-Mediated Inhibition of HIV-1-Infected Autologous CD4+ T Cells. J Virol. 2016;90:4441-4453 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Tang C, Sun H, Kadoki M, Han W, Ye X, Makusheva Y, et al. Blocking Dectin-1 prevents colorectal tumorigenesis by suppressing prostaglandin E2 production in myeloid-derived suppressor cells and enhancing IL-22 binding protein expression. Nat Commun. 2023;14:1493 pubmed publisher
Fehlberg L, Guadagnin A, Thomas B, Ballou M, Loor J, Sugimoto Y, et al. Feeding rumen-protected lysine altered immune and metabolic biomarkers in dairy cows during the transition period. J Dairy Sci. 2023;106:2989-3007 pubmed publisher
Vondra S, H xf6 bler A, Lackner A, Raffetseder J, Mihalic Z, Vogel A, et al. The human placenta shapes the phenotype of decidual macrophages. Cell Rep. 2023;42:111977 pubmed publisher
Yokoyama K, Mitoma H, Kawano S, Yamauchi Y, Wang Q, Ayano M, et al. CEACAM 1, 3, 5 and 6 -positive classical monocytes correlate with interstitial lung disease in early systemic sclerosis. Front Immunol. 2022;13:1016914 pubmed publisher
Takele Y, Adem E, Franssen S, Womersley R, Kaforou M, Levin M, et al. Impaired in vitro Interferon-γ production in patients with visceral leishmaniasis is improved by inhibition of PD1/PDL-1 ligation. PLoS Negl Trop Dis. 2022;16:e0010544 pubmed publisher
Guo Z, Yuan Y, Chen C, Lin J, Ma Q, Liu G, et al. Durable complete response to neoantigen-loaded dendritic-cell vaccine following anti-PD-1 therapy in metastatic gastric cancer. NPJ Precis Oncol. 2022;6:34 pubmed publisher
Rhoades N, Davies M, Lewis S, Cinco I, Kohama S, Bermudez L, et al. Functional, transcriptional, and microbial shifts associated with healthy pulmonary aging in rhesus macaques. Cell Rep. 2022;39:110725 pubmed publisher
Duncan C, Skouboe M, Howarth S, Hollensen A, Chen R, B xf8 rresen M, et al. Life-threatening viral disease in a novel form of autosomal recessive IFNAR2 deficiency in the Arctic. J Exp Med. 2022;219: pubmed publisher
Tähtinen S, Tong A, Himmels P, Oh J, Paler Martinez A, Kim L, et al. IL-1 and IL-1ra are key regulators of the inflammatory response to RNA vaccines. Nat Immunol. 2022;23:532-542 pubmed publisher
Thomas B, Guadagnin A, Fehlberg L, Sugimoto Y, Shinzato I, Drackley J, et al. Feeding rumen-protected lysine to dairy cows prepartum improves performance and health of their calves. J Dairy Sci. 2022;105:2256-2274 pubmed publisher
Zhu Z, Tang N, Wang M, Zhou J, Wang J, Ren H, et al. Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma. Front Immunol. 2021;12:781087 pubmed publisher
Weiss R, Gerdes W, Berthold R, Sack U, Koehl U, Hauschildt S, et al. Comparison of Three CD3-Specific Separation Methods Leading to Labeled and Label-Free T Cells. Cells. 2021;10: pubmed publisher
Downes D, Cross A, Hua P, Roberts N, Schwessinger R, Cutler A, et al. Identification of LZTFL1 as a candidate effector gene at a COVID-19 risk locus. Nat Genet. 2021;53:1606-1615 pubmed publisher
Claus C, Fritz R, Schilling E, Reibetanz U. The Metabolic Response of Various Cell Lines to Microtubule-Driven Uptake of Lipid- and Polymer-Coated Layer-by-Layer Microcarriers. Pharmaceutics. 2021;13: pubmed publisher
Wegner J, Zillinger T, Schlee Guimaraes T, Bartok E, Schlee M. An epigenetic GPI anchor defect impairs TLR4 signaling in the B cell transdifferentiation model for primary human monocytes BLaER1. Sci Rep. 2021;11:14983 pubmed publisher
Ferrari M, Mekkaoui L, Ilca F, Akbar Z, Bughda R, Lamb K, et al. Characterization of a Novel ACE2-Based Therapeutic with Enhanced Rather than Reduced Activity against SARS-CoV-2 Variants. J Virol. 2021;95:e0068521 pubmed publisher
da Rocha Sobrinho H, Saar Gomes R, da Silva D, Quixabeira V, Joosten L, Ribeiro De Barros Cardoso C, et al. Toll-like receptor 10 controls TLR2-induced cytokine production in monocytes from patients with Parkinson's disease. J Neurosci Res. 2021;99:2511-2524 pubmed publisher
Magg T, Okano T, Koenig L, Boehmer D, Schwartz S, Inoue K, et al. Heterozygous OAS1 gain-of-function variants cause an autoinflammatory immunodeficiency. Sci Immunol. 2021;6: pubmed publisher
Lopes M, Alharthi A, Lopreiato V, Abdel Hamied E, Liang Y, Coleman D, et al. Maternal supplementation with cobalt sources, folic acid, and rumen-protected methionine and its effects on molecular and functional correlates of the immune system in neonatal Holstein calves. J Dairy Sci. 2021;104:9340-9354 pubmed publisher
Mu Z, Wei W, Fair B, Miao J, Zhu P, Li Y. The impact of cell type and context-dependent regulatory variants on human immune traits. Genome Biol. 2021;22:122 pubmed publisher
Bade R, Schehr J, Emamekhoo H, Gibbs B, Rodems T, Mannino M, et al. Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma. Mol Oncol. 2021;15:2330-2344 pubmed publisher
Alhaj Hussen K, Michonneau D, Biajoux V, Keita S, Dubouchet L, Nelson E, et al. CD4+CD8+ T-Lymphocytes in Xenogeneic and Human Graft-versus-Host Disease. Front Immunol. 2020;11:579776 pubmed publisher
Mahmud F, Du Y, Greif E, Boucher T, Dannals R, Mathews W, et al. Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection. J Neuroinflammation. 2020;17:273 pubmed publisher
Miksiunas R, Rucinskas K, Janusauskas V, Labeit S, Bironaite D. Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Improves Energetic Status and Cardiomyogenic Differentiation of Human Dilated Myocardium-Derived Primary Mesenchymal Cells. Int J Mol Sci. 2020;21: pubmed publisher
Constantinescu Bercu A, Grassi L, Frontini M, Salles Crawley I, Woollard K, Crawley J. Activated αIIbβ3 on platelets mediates flow-dependent NETosis via SLC44A2. elife. 2020;9: pubmed publisher
Lavaert M, Liang K, Vandamme N, Park J, Roels J, Kowalczyk M, et al. Integrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes. Immunity. 2020;52:1088-1104.e6 pubmed publisher
Jankeel A, Menicucci A, Woolsey C, Fenton K, Mendoza N, Versteeg K, et al. Early Transcriptional Changes within Liver, Adrenal Gland, and Lymphoid Tissues Significantly Contribute to Ebola Virus Pathogenesis in Cynomolgus Macaques. J Virol. 2020;94: pubmed publisher
Woolsey C, Jankeel A, Matassov D, Geisbert J, Agans K, Borisevich V, et al. Immune correlates of postexposure vaccine protection against Marburg virus. Sci Rep. 2020;10:3071 pubmed publisher
Kedage V, Ellerman D, Chen Y, Liang W, Borneo J, Wu Y, et al. Harnessing MerTK agonism for targeted therapeutics. MAbs. 2020;12:1685832 pubmed publisher
Woolsey C, Menicucci A, Cross R, Luthra P, Agans K, Borisevich V, et al. A VP35 Mutant Ebola Virus Lacks Virulence but Can Elicit Protective Immunity to Wild-Type Virus Challenge. Cell Rep. 2019;28:3032-3046.e6 pubmed publisher
Alharthi A, Lopreiato V, Dai H, Bucktrout R, Abdelmegeid M, Batistel F, et al. Short communication: Supply of methionine during late pregnancy enhances whole-blood innate immune response of Holstein calves partly through changes in mRNA abundance in polymorphonuclear leukocytes. J Dairy Sci. 2019;102:10599-10605 pubmed publisher
Ferguson Bennit H, Gonda A, Oppegard L, Chi D, Khan S, Wall N. Uptake of lymphoma-derived exosomes by peripheral blood leukocytes. Blood Lymphat Cancer. 2017;7:9-23 pubmed publisher
Broeren M, Waterborg C, Wiegertjes R, Thurlings R, Koenders M, van Lent P, et al. A three-dimensional model to study human synovial pathology. ALTEX. 2019;36:18-28 pubmed publisher
Zhou Z, Ferdous F, Montagner P, Luchini D, Corrêa M, Loor J. Methionine and choline supply during the peripartal period alter polymorphonuclear leukocyte immune response and immunometabolic gene expression in Holstein cows. J Dairy Sci. 2018;101:10374-10382 pubmed publisher
Kiers D, Wielockx B, Peters E, van Eijk L, Gerretsen J, John A, et al. Short-Term Hypoxia Dampens Inflammation in vivo via Enhanced Adenosine Release and Adenosine 2B Receptor Stimulation. EBioMedicine. 2018;33:144-156 pubmed publisher
Powell E, Reinhardt T, Casas E, Lippolis J. The effect of pegylated granulocyte colony-stimulating factor treatment prior to experimental mastitis in lactating Holsteins. J Dairy Sci. 2018;101:8182-8193 pubmed publisher
Woodberry T, Loughland J, Minigo G, Burel J, Amante F, Piera K, et al. Early Immune Regulatory Changes in a Primary Controlled Human Plasmodium vivax Infection: CD1c+ Myeloid Dendritic Cell Maturation Arrest, Induction of the Kynurenine Pathway, and Regulatory T Cell Activation. Infect Immun. 2017;85: pubmed publisher
Wang H, Gui H, Rallo M, Xu Z, Matise M. Atrophin protein RERE positively regulates Notch targets in the developing vertebrate spinal cord. J Neurochem. 2017;141:347-357 pubmed publisher
Spildrejorde M, Bartlett R, Stokes L, Jalilian I, Peranec M, Sluyter V, et al. R270C polymorphism leads to loss of function of the canine P2X7 receptor. Physiol Genomics. 2014;46:512-22 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
product information
Antigen :
CD14
Apps. Abbrev. :
FC
Cat # :
301807
Clone :
M5E2
Item :
APC anti-human CD14
Isotype :
Mouse IgG2a, κ
Other Names :
LPS receptor
Size :
25 tests
Price (USD) :
65 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Immunogen :
Full-length human CD14 protein
Application Notes :
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation^4, and immunofluorescence microscopy^5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA