Alexa Fluor® 647 anti-human CD11c | BioLegend 301620 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Alexa Fluor® 647 anti-human CD11c

catalog :

301620

quantity :

100 tests

price :

265 USD

clonality :

monoclonal

host :

mouse

conjugate :

AF647

clone name :

3.9

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Singh A, Kazer S, Roider J, Krista K, Millar J, Asowata O, et al. Innate Lymphoid Cell Activation and Sustained Depletion in Blood and Tissue of Children Infected with HIV from Birth Despite Antiretroviral Therapy. Cell Rep. 2020;32:108153 pubmed publisher
	Murakami T, Kim J, Li Y, Green G, Shikanov A, Ono A. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Nat Commun. 2018;9:2436 pubmed publisher
	Sprokholt J, Heineke M, Kaptein T, van Hamme J, Geijtenbeek T. DCs facilitate B cell responses against microbial DNA via DC-SIGN. PLoS ONE. 2017;12:e0185580 pubmed publisher
	Sándor N, Kristóf K, Paréj K, Pap D, Erdei A, Bajtay Z. CR3 is the dominant phagocytotic complement receptor on human dendritic cells. Immunobiology. 2013;218:652-63 pubmed publisher
	Paulson K, Zhu S, Chen M, Nurmohamed S, Jongstra Bilen J, Cybulsky M. Resident intimal dendritic cells accumulate lipid and contribute to the initiation of atherosclerosis. Circ Res. 2010;106:383-90 pubmed publisher
	Asai A, Okajima F, Nakagawa K, Ibusuki D, Tanimura K, Nakajima Y, et al. Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1. J Lipid Res. 2009;50:957-65 pubmed publisher
	Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, et al. Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008;112:1231-9 pubmed publisher
	Sadhu C, Ting H, Lipsky B, Hensley K, Garcia Martinez L, Simon S, et al. CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007;81:1395-403 pubmed

product information

Antigen :

CD11c

Apps. Abbrev. :

Flow Cytometry

Cat # :

301620

Clone :

3.9

Item :

Alexa Fluor® 647 anti-human CD11c

Isotype :

Mouse IgG1, κ

Other Names :

Integrin αX subunit, CR4, p150, ITGAX

Size :

100 tests

Price (USD) :

265 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

A647

Application Notes :

Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent 12 . While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 4 , and functional assays 5,6 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).

more info or order :

BioLegend product webpage