LEAF™ Purified anti-human CD11c | BioLegend 301616 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD11c

catalog :

301616

quantity :

500 μg

price :

350 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3.9

reactivity :

human, chimpanzee

application :

immunohistochemistry, neutralization, flow cytometry, blocking or activating experiments

citations: 7

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; fig 4a flow cytometry; human; fig 1a	Buffone A, Anderson N, Hammer D. Migration against the direction of flow is LFA-1-dependent in human hematopoietic stem and progenitor cells. J Cell Sci. 2018;131: pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Michlmayr D, Kim E, Rahman A, Raghunathan R, Kim Schulze S, Che Y, et al. Comprehensive Immunoprofiling of Pediatric Zika Reveals Key Role for Monocytes in the Acute Phase and No Effect of Prior Dengue Virus Infection. Cell Rep. 2020;31:107569 pubmed publisher
	Larsson K, Kock A, Idborg H, Arsenian Henriksson M, Martinsson T, Johnsen J, et al. COX/mPGES-1/PGE2 pathway depicts an inflammatory-dependent high-risk neuroblastoma subset. Proc Natl Acad Sci U S A. 2015;112:8070-5 pubmed publisher
	Asai A, Okajima F, Nakagawa K, Ibusuki D, Tanimura K, Nakajima Y, et al. Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1. J Lipid Res. 2009;50:957-65 pubmed publisher
	Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, et al. Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses. Blood. 2008;112:1231-9 pubmed publisher
	Sadhu C, Ting H, Lipsky B, Hensley K, Garcia Martinez L, Simon S, et al. CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007;81:1395-403 pubmed

product information

Antigen :

CD11c

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Block, IHC

Cat # :

301616

Clone :

3.9

Item :

LEAF™ Purified anti-human CD11c

Isotype :

Mouse IgG1, κ

Other Names :

Integrin αX subunit, CR4, p150, ITGAX

Size :

500 μg

Price (USD) :

350 USD

Reactivity :

Human, African Green, Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent 12 . While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections 4 , and functional assays 5,6 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).