product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD11b
catalog :
301302
quantity :
100 μg
price :
50 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ICRF44
reactivity :
human, chimpanzee, rhesus macaque
application :
immunohistochemistry, immunocytochemistry, flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 60
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2-5
Jakob M, Hambrecht M, Spiegel J, Kitz J, Canis M, Dressel R, et al. Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Show Comparable Functionality to Their Autologous Origin. Cells. 2020;10: pubmed publisher
  • flow cytometry; human; 1:50; loading ...
Noz M, Bekkering S, Groh L, Nielen T, Lamfers E, Schlitzer A, et al. Reprogramming of bone marrow myeloid progenitor cells in patients with severe coronary artery disease. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig 2f
Sueyoshi K, Ledderose C, Shen Y, Lee A, Shapiro N, Junger W. Lipopolysaccharide suppresses T cells by generating extracellular ATP that impairs their mitochondrial function via P2Y11 receptors. J Biol Chem. 2019;294:6283-6293 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Kennedy J, Steain M, Slobedman B, Abendroth A. Infection and Functional Modulation of Human Monocytes and Macrophages by Varicella-Zoster Virus. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Buchrieser J, Oliva Martin M, Moore M, Long J, Cowley S, Perez Simon J, et al. RIPK1 is a critical modulator of both tonic and TLR-responsive inflammatory and cell death pathways in human macrophage differentiation. Cell Death Dis. 2018;9:973 pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Tamassia N, Arruda Silva F, Calzetti F, Lonardi S, Gasperini S, Gardiman E, et al. A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It. Front Immunol. 2018;9:795 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Fujisaka Y, Iwata T, Tamai K, Nakamura M, Mochizuki M, Shibuya R, et al. Long non-coding RNA HOTAIR up-regulates chemokine (C-C motif) ligand 2 and promotes proliferation of macrophages and myeloid-derived suppressor cells in hepatocellular carcinoma cell lines. Oncol Lett. 2018;15:509-514 pubmed publisher
  • flow cytometry; human; fig 1a
Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  • flow cytometry; human; loading ...; tbl 2
Bzowska M, Nogieć A, Bania K, Zygmunt M, Zarebski M, Dobrucki J, et al. Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages. J Leukoc Biol. 2017;102:763-774 pubmed publisher
  • mass cytometry; human; loading ...; fig s3a
See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
  • immunohistochemistry; human; fig 2a
Zhang J, Xu X, Shi M, Chen Y, Yu D, Zhao C, et al. CD13hi Neutrophil-like myeloid-derived suppressor cells exert immune suppression through Arginase 1 expression in pancreatic ductal adenocarcinoma. Oncoimmunology. 2017;6:e1258504 pubmed publisher
  • flow cytometry; human; fig 3a
Janesch P, Rouha H, Weber S, Malafa S, Gross K, Maierhofer B, et al. Selective sensitization of human neutrophils to LukGH mediated cytotoxicity by Staphylococcus aureus and IL-8. J Infect. 2017;74:473-483 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Serr I, Fürst R, Ott V, Scherm M, Nikolaev A, Gökmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
  • flow cytometry; rhesus macaque; tbl 1
Williams D, Engle E, Shirk E, Queen S, Gama L, Mankowski J, et al. Splenic Damage during SIV Infection: Role of T-Cell Depletion and Macrophage Polarization and Infection. Am J Pathol. 2016;186:2068-2087 pubmed publisher
  • blocking or activating experiments; human; 50 ug/ml; loading ...; fig 2b
Schneider A, Sándor N, Kárpáti Ã, Józsi M. Complement factor H modulates the activation of human neutrophil granulocytes and the generation of neutrophil extracellular traps. Mol Immunol. 2016;72:37-48 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Curtis B, Roman A, Sullivan M, Raven C, Larison J, Weitekamp L. Two cases of maternal alloimmunization against human neutrophil alloantigen-4b, one causing severe alloimmune neonatal neutropenia. Transfusion. 2016;56:101-6 pubmed publisher
  • immunohistochemistry; human; 1:200; tbl s4
Larsson K, Kock A, Idborg H, Arsenian Henriksson M, Martinsson T, Johnsen J, et al. COX/mPGES-1/PGE2 pathway depicts an inflammatory-dependent high-risk neuroblastoma subset. Proc Natl Acad Sci U S A. 2015;112:8070-5 pubmed publisher
  • flow cytometry; human; fig 6
Hünniger K, Bieber K, Martin R, Lehnert T, Figge M, Löffler J, et al. A second stimulus required for enhanced antifungal activity of human neutrophils in blood is provided by anaphylatoxin C5a. J Immunol. 2015;194:1199-210 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Chen X, Schneewind O, Missiakas D. Engineered human antibodies for the opsonization and killing of Staphylococcus aureus. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Supper E, Rudat S, Iyer V, Droop A, Wong K, Spinella J, et al. Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia. Nat Commun. 2021;12:2482 pubmed publisher
Hemkemeyer S, Vollmer V, Schwarz V, Lohmann B, Honnert U, Taha M, et al. Local Myo9b RhoGAP activity regulates cell motility. J Biol Chem. 2021;296:100136 pubmed publisher
Ryu S, Lee E, Kim D, Kim Y, Chung D, Kim J, et al. Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis. Arthritis Res Ther. 2020;22:63 pubmed publisher
Paubelle E, Zylbersztejn F, Maciel T, Carvalho C, Mupo A, Cheok M, et al. Vitamin D Receptor Controls Cell Stemness in Acute Myeloid Leukemia and in Normal Bone Marrow. Cell Rep. 2020;30:739-754.e4 pubmed publisher
Ross N, Lohmann F, Carbonneau S, Fazal A, Weihofen W, Gleim S, et al. CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma. Nat Chem Biol. 2020;16:50-59 pubmed publisher
Dutertre C, Becht E, Irac S, Khalilnezhad A, Narang V, Khalilnezhad S, et al. Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells. Immunity. 2019;51:573-589.e8 pubmed publisher
Hasselmann J, Coburn M, England W, Figueroa Velez D, Kiani Shabestari S, Tu C, et al. Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo. Neuron. 2019;103:1016-1033.e10 pubmed publisher
Ristow L, Tran V, Schwartz K, Pankratz L, Mehle A, Sauer J, et al. The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity. MBio. 2019;10: pubmed publisher
van Bergeijk P, Seneviratne U, Aparicio Prat E, Stanton R, Hasson S. SRSF1 and PTBP1 are trans-acting factors that suppress the formation of a CD33 splicing isoform linked to Alzheimer's disease risk. Mol Cell Biol. 2019;: pubmed publisher
Han L, Zhang Q, Dail M, Shi C, Cavazos A, Ruvolo V, et al. Concomitant targeting of BCL2 with venetoclax and MAPK signaling with cobimetinib in acute myeloid leukemia models. Haematologica. 2019;: pubmed publisher
Pohlmeyer C, Gonzalez V, Irrinki A, Ramirez R, Li L, Mulato A, et al. Identification of NK Cell Subpopulations That Differentiate HIV-Infected Subject Cohorts with Diverse Levels of Virus Control. J Virol. 2019;93: pubmed publisher
Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed publisher
Saggu G, Okubo K, Chen Y, Vattepu R, Tsuboi N, Rosetti F, et al. Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment. Nat Commun. 2018;9:5058 pubmed publisher
Wang W, Marinis J, Beal A, Savadkar S, Wu Y, Khan M, et al. RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer. Cancer Cell. 2018;34:757-774.e7 pubmed publisher
Agrawal N, Streaţă I, Pei G, Weiner J, Kotze L, Bandermann S, et al. Human Monocytic Suppressive Cells Promote Replication of Mycobacterium tuberculosis and Alter Stability of in vitro Generated Granulomas. Front Immunol. 2018;9:2417 pubmed publisher
Tseng H, Samarelli A, Kammerer P, Scholze S, Ziegler T, Immler R, et al. LCP1 preferentially binds clasped αMβ2 integrin and attenuates leukocyte adhesion under flow. J Cell Sci. 2018;131: pubmed publisher
Piragyte I, Clapes T, Polyzou A, Klein Geltink R, Lefkopoulos S, Yin N, et al. A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways. Nat Commun. 2018;9:3090 pubmed publisher
Zhao Y, Zhong L, Liu L, Yao S, Chen M, Li L, et al. Salinomycin induces apoptosis and differentiation in human acute promyelocytic leukemia cells. Oncol Rep. 2018;40:877-886 pubmed publisher
Ran L, Zeng Y, Wang S, Zhang D, Hong M, Li S, et al. Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells. Mol Med Rep. 2018;18:723-732 pubmed publisher
Tseng A, Kim K, Li J, Cho J. Myeloperoxidase Negatively Regulates Neutrophil-Endothelial Cell Interactions by Impairing αMβ2 Integrin Function in Sterile Inflammation. Front Med (Lausanne). 2018;5:134 pubmed publisher
Khajuria R, Munschauer M, Ulirsch J, Fiorini C, Ludwig L, McFarland S, et al. Ribosome Levels Selectively Regulate Translation and Lineage Commitment in Human Hematopoiesis. Cell. 2018;173:90-103.e19 pubmed publisher
Cao Q, Wang Y, Wang C, Wang X, Lee V, Zheng G, et al. Therapeutic potential of regulatory macrophages generated from peritoneal dialysate in adriamycin nephropathy. Am J Physiol Renal Physiol. 2018;314:F561-F571 pubmed publisher
Li N, Yang H, Wang M, Lü S, Zhang Y, Long M. Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling. Mol Biol Cell. 2018;29:408-418 pubmed publisher
Vargas Hernandez A, Mace E, Zimmerman O, Zerbe C, Freeman A, Rosenzweig S, et al. Ruxolitinib partially reverses functional natural killer cell deficiency in patients with signal transducer and activator of transcription 1 (STAT1) gain-of-function mutations. J Allergy Clin Immunol. 2018;141:2142-2155.e5 pubmed publisher
Wang Z, Thinn A, Zhu J. A pivotal role for a conserved bulky residue at the α1-helix of the αI integrin domain in ligand binding. J Biol Chem. 2017;292:20756-20768 pubmed publisher
Bracq L, Xie M, Lambelé M, Vu L, Matz J, Schmitt A, et al. T Cell-Macrophage Fusion Triggers Multinucleated Giant Cell Formation for HIV-1 Spreading. J Virol. 2017;91: pubmed publisher
Benedek G, Meza Romero R, Jordan K, Zhang Y, Nguyen H, Kent G, et al. MIF and D-DT are potential disease severity modifiers in male MS subjects. Proc Natl Acad Sci U S A. 2017;114:E8421-E8429 pubmed publisher
Gov L, Schneider C, Lima T, Pandori W, Lodoen M. NLRP3 and Potassium Efflux Drive Rapid IL-1β Release from Primary Human Monocytes during Toxoplasma gondii Infection. J Immunol. 2017;199:2855-2864 pubmed publisher
Wen T, Kuhl J, Putnam P, Mukkada V, Farrell M, Kaul A, et al. A flow cytometry-based diagnosis of eosinophilic esophagitis. J Allergy Clin Immunol. 2017;140:1736-1739.e3 pubmed publisher
Cobb D, Kim O, Golden Mason L, Rosen H, Hahn Y. Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection. Hepatology. 2017;: pubmed publisher
Patel A, Zhang Y, Fullerton J, Boelen L, Rongvaux A, Maini A, et al. The fate and lifespan of human monocyte subsets in steady state and systemic inflammation. J Exp Med. 2017;214:1913-1923 pubmed publisher
Yip B, Steeples V, Repapi E, Armstrong R, Llorian M, Roy S, et al. The U2AF1S34F mutation induces lineage-specific splicing alterations in myelodysplastic syndromes. J Clin Invest. 2017;127:2206-2221 pubmed publisher
Abud E, Ramirez R, Martinez E, Healy L, Nguyen C, Newman S, et al. iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases. Neuron. 2017;94:278-293.e9 pubmed publisher
Lu X, Horner J, Paul E, Shang X, Troncoso P, Deng P, et al. Effective combinatorial immunotherapy for castration-resistant prostate cancer. Nature. 2017;543:728-732 pubmed publisher
Zhou H, Mak P, Mu H, Mak D, Zeng Z, Cortes J, et al. Combined inhibition of ?-catenin and Bcr-Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo. Leukemia. 2017;31:2065-2074 pubmed publisher
Lu Y, Liu H, Bi Y, Yang H, Li Y, Wang J, et al. Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1α-dependent glycolysis. Cell Mol Immunol. 2018;15:618-629 pubmed publisher
Wen T, Mingler M, Wahl B, Khorki M, Pabst O, Zimmermann N, et al. Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils. J Immunol. 2014;192:5481-9 pubmed publisher
Söderholm N, Vielfort K, Hultenby K, Aro H. Pathogenic Neisseria hitchhike on the uropod of human neutrophils. PLoS ONE. 2011;6:e24353 pubmed publisher
Dixit N, Yamayoshi I, Nazarian A, Simon S. Migrational guidance of neutrophils is mechanotransduced via high-affinity LFA-1 and calcium flux. J Immunol. 2011;187:472-81 pubmed publisher
Kanda H, Newton R, Klein R, Morita Y, Gunn M, Rosen S. Autotaxin, an ectoenzyme that produces lysophosphatidic acid, promotes the entry of lymphocytes into secondary lymphoid organs. Nat Immunol. 2008;9:415-23 pubmed publisher
product information
Antigen :
CD11b
Apps. Abbrev. :
Flow Cytometry, CyTOF®, ICC, IHC
Cat # :
301302
Clone :
ICRF44
Item :
Purified anti-human CD11b
Isotype :
Mouse IgG1, κ
Other Names :
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Size :
100 μg
Price (USD) :
50 USD
Reactivity :
Human, African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Swine (Pig, Porcine)
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy 5 , stimulation of monocytes 3 , blocking of heterotypic PMN aggregation 8 , and blocking of granulocyte activation 12 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. Nos. 301361 301362).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA