product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD8a
catalog :
301074
quantity :
1 mg
price :
715 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RPA-T8
reactivity :
human, mouse, rhesus macaque
application :
immunohistochemistry, flow cytometry, mass cytometry
more info or order :
citations: 24
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 2b
Jin Y, Lorvik K, Jin Y, Beck C, Sike A, Persiconi I, et al. Development of STEAP1 targeting chimeric antigen receptor for adoptive cell therapy against cancer. Mol Ther Oncolytics. 2022;26:189-206 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed publisher
  • flow cytometry; human; loading ...; fig 2e, 6d
Hickman T, Choi E, Whiteman K, Muralidharan S, Pai T, Johnson T, et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE. 2022;17:e0266980 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Park C, Kehrl J. An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph node. elife. 2019;8: pubmed publisher
  • mass cytometry; human; loading ...
Banga R, Rebecchini C, Procopio F, Noto A, Munoz O, Ioannidou K, et al. Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement. PLoS Pathog. 2019;15:e1007918 pubmed publisher
  • flow cytometry; human; loading ...; fig 3f, 3g
Fransen N, Crusius J, Smolders J, Mizee M, Van Eden C, Luchetti S, et al. Post-mortem multiple sclerosis lesion pathology is influenced by single nucleotide polymorphisms. Brain Pathol. 2020;30:106-119 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Terahara K, Iwabuchi R, Hosokawa M, Nishikawa Y, Takeyama H, Takahashi Y, et al. A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro. BMC Res Notes. 2019;12:242 pubmed publisher
  • flow cytometry; human; loading ...; fig ex5e
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Muller Durovic B, Grählert J, Devine O, Akbar A, Hess C. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age. Aging (Albany NY). 2019;11:724-740 pubmed publisher
Wienert B, Nguyen D, Guenther A, Feng S, Locke M, Wyman S, et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun. 2020;11:2109 pubmed publisher
Hudson W, Gensheimer J, Hashimoto M, Wieland A, Valanparambil R, Li P, et al. Proliferating Transitory T Cells with an Effector-like Transcriptional Signature Emerge from PD-1+ Stem-like CD8+ T Cells during Chronic Infection. Immunity. 2019;51:1043-1058.e4 pubmed publisher
Geng J, Raghavan M. CD8αα homodimers function as a coreceptor for KIR3DL1. Proc Natl Acad Sci U S A. 2019;116:17951-17956 pubmed publisher
Yang T, St John L, Garber H, Kerros C, Ruisaard K, Clise Dwyer K, et al. Membrane-Associated Proteinase 3 on Granulocytes and Acute Myeloid Leukemia Inhibits T Cell Proliferation. J Immunol. 2018;201:1389-1399 pubmed publisher
Mavigner M, Habib J, Deléage C, Rosen E, Mattingly C, Bricker K, et al. Simian Immunodeficiency Virus Persistence in Cellular and Anatomic Reservoirs in Antiretroviral Therapy-Suppressed Infant Rhesus Macaques. J Virol. 2018;92: pubmed publisher
Martins M, Tully D, Pedreño Lopez N, von Bredow B, Pauthner M, Shin Y, et al. Mamu-B*17+ Rhesus Macaques Vaccinated with env, vif, and nef Manifest Early Control of SIVmac239 Replication. J Virol. 2018;92: pubmed publisher
Jegaskanda S, Mason R, Andrews S, Wheatley A, Zhang R, Reynoso G, et al. Intranasal Live Influenza Vaccine Priming Elicits Localized B Cell Responses in Mediastinal Lymph Nodes. J Virol. 2018;92: pubmed publisher
Böiers C, Richardson S, Laycock E, Zriwil A, Turati V, Brown J, et al. A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1. Dev Cell. 2018;44:362-377.e7 pubmed publisher
Pernas M, Tarancón Díez L, Rodríguez Gallego E, Gomez J, Prado J, Casado C, et al. Factors Leading to the Loss of Natural Elite Control of HIV-1 Infection. J Virol. 2018;92: pubmed publisher
Younan P, Iampietro M, Nishida A, Ramanathan P, Santos R, Dutta M, et al. Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm. MBio. 2017;8: pubmed publisher
Teigler J, Zelinskyy G, Eller M, Bolton D, Marovich M, Gordon A, et al. Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection. J Virol. 2017;91: pubmed publisher
Horwitz J, Bar On Y, Lu C, Fera D, Lockhart A, Lorenzi J, et al. Non-neutralizing Antibodies Alter the Course of HIV-1 Infection In Vivo. Cell. 2017;170:637-648.e10 pubmed publisher
Termini J, Magnani D, Maxwell H, Lauer W, Castro I, Pecotte J, et al. Simian T Lymphotropic Virus 1 Infection of Papio anubis: tax Sequence Heterogeneity and T Cell Recognition. J Virol. 2017;91: pubmed publisher
product information
Antigen :
CD8alpha
Apps. Abbrev. :
FC, CyTOF®, Costim, IHC
Cat # :
301074
Clone :
RPA-T8
Item :
Ultra-LEAF™ Purified anti-human CD8a
Isotype :
Mouse IgG1, κ
Other Names :
T8, Leu2
Size :
1 mg
Price (USD) :
715 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Application Notes :
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections^3 and costimulation of T cell responses^4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA