product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 510™ anti-human CD8a
catalog :
301048
quantity :
100 tests
price :
380 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV510
clone name :
RPA-T8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 58
Published Application/Species/Sample/DilutionReference
  • flow cytometry; rhesus macaque; loading ...
Vaccari M, Gordon S, Fourati S, Schifanella L, Liyanage N, Cameron M, et al. Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition. Nat Med. 2016;22:762-70 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Saotome K, Dudgeon D, Colotti K, Moore M, Jones J, Zhou Y, et al. Structural analysis of cancer-relevant TCR-CD3 and peptide-MHC complexes by cryoEM. Nat Commun. 2023;14:2401 pubmed publisher
Pinto C, Slavic Obradovic K, F xfc rweger D, Thaler B, Souabni A, Carotta S, et al. Tumor microenvironment mimicking 3D models unveil the multifaceted effects of SMAC mimetics. iScience. 2023;26:106381 pubmed publisher
Rosain J, Neehus A, Manry J, Yang R, Le Pen J, Daher W, et al. Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria. Cell. 2023;186:621-645.e33 pubmed publisher
Zhang B, Sun J, Yuan Y, Ji D, Sun Y, Liu Y, et al. Proximity-enabled covalent binding of IL-2 to IL-2Rα selectively activates regulatory T cells and suppresses autoimmunity. Signal Transduct Target Ther. 2023;8:28 pubmed publisher
Du Bruyn E, Stek C, Daroowala R, Said Hartley Q, Hsiao M, Schäfer G, et al. Effects of tuberculosis and/or HIV-1 infection on COVID-19 presentation and immune response in Africa. Nat Commun. 2023;14:188 pubmed publisher
Keeton R, Tincho M, Suzuki A, Benede N, Ngomti A, Baguma R, et al. Impact of SARS-CoV-2 exposure history on the T cell and IgG response. Cell Rep Med. 2023;4:100898 pubmed publisher
Lima N, Musayev M, Johnston T, Wagner D, Henry A, Wang L, et al. Primary exposure to SARS-CoV-2 variants elicits convergent epitope specificities, immunoglobulin V gene usage and public B cell clones. Nat Commun. 2022;13:7733 pubmed publisher
Ellis G, Deng M, Winn D, Coker K, Shukla D, Bhoj V, et al. Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation. STAR Protoc. 2022;3:101784 pubmed publisher
Liisborg C. Age-related macular degeneration and myeloproliferative neoplasms - A common pathway. Acta Ophthalmol. 2022;100 Suppl 271:3-35 pubmed publisher
Liisborg C, Skov V, Kj xe6 r L, Hasselbalch H, Lykke S xf8 rensen T. Lower CXCR3 expression in both patients with neovascular AMD and advanced stages of chronic myeloproliferative blood cancers. PLoS ONE. 2022;17:e0269960 pubmed publisher
Koutsakos M, Lee W, Reynaldi A, Tan H, Gare G, Kinsella P, et al. The magnitude and timing of recalled immunity after breakthrough infection is shaped by SARS-CoV-2 variants. Immunity. 2022;55:1316-1326.e4 pubmed publisher
Sudmeier L, Hoang K, Nduom E, Wieland A, Neill S, Schniederjan M, et al. Distinct phenotypic states and spatial distribution of CD8+ T cell clonotypes in human brain metastases. Cell Rep Med. 2022;3:100620 pubmed publisher
Ellis G, Coker K, Winn D, Deng M, Shukla D, Bhoj V, et al. Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque. Cell Rep Med. 2022;3:100614 pubmed publisher
Ma K, Schonnesen A, He C, Xia A, Sun E, Chen E, et al. High-throughput and high-dimensional single-cell analysis of antigen-specific CD8+ T cells. Nat Immunol. 2021;22:1590-1598 pubmed publisher
Moreno Fernandez M, Damen M, Divanovic S. A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies. STAR Protoc. 2021;2:100937 pubmed publisher
Keeton R, Richardson S, Moyo Gwete T, Hermanus T, Tincho M, Benede N, et al. Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner. Cell Host Microbe. 2021;29:1611-1619.e5 pubmed publisher
Cho J, Park S, Kim G, Han H, Shim H, Shin S, et al. Dysregulation of TFH-B-TRM lymphocyte cooperation is associated with unfavorable anti-PD-1 responses in EGFR-mutant lung cancer. Nat Commun. 2021;12:6068 pubmed publisher
Wheatley A, Pymm P, Esterbauer R, Dietrich M, Lee W, Drew D, et al. Landscape of human antibody recognition of the SARS-CoV-2 receptor binding domain. Cell Rep. 2021;37:109822 pubmed publisher
Townsley S, Leggat D, Prabhakaran M, McDermott A, Krebs S. Sequential staining of HIV gp140 to capture antigen-specific human B cells via flow cytometry. STAR Protoc. 2021;2:100771 pubmed publisher
Nakano M, Ayano M, Kushimoto K, Kawano S, Higashioka K, Inokuchi S, et al. Increased Proportion of CD226+ B Cells Is Associated With the Disease Activity and Prognosis of Systemic Lupus Erythematosus. Front Immunol. 2021;12:713225 pubmed publisher
Wang L, Zhou T, Zhang Y, Yang E, Schramm C, Shi W, et al. Ultrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants. Science. 2021;: pubmed publisher
Tan J, Cho H, Pholcharee T, Pereira L, Doumbo S, Doumtabe D, et al. Functional human IgA targets a conserved site on malaria sporozoites. Sci Transl Med. 2021;13: pubmed publisher
Pušnik J, Richter E, Schulte B, Dolscheid Pommerich R, Bode C, Putensen C, et al. Memory B cells targeting SARS-CoV-2 spike protein and their dependence on CD4+ T cell help. Cell Rep. 2021;35:109320 pubmed publisher
Nguyen T, Rowntree L, Petersen J, Chua B, Hensen L, Kedzierski L, et al. CD8+ T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuity. Immunity. 2021;54:1066-1082.e5 pubmed publisher
Lu Y, Li Y, Liu Q, Tian N, Du P, Zhu F, et al. MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment. Gastroenterology. 2021;161:575-591.e16 pubmed publisher
Mukhamedova M, Wrapp D, Shen C, Gilman M, Ruckwardt T, Schramm C, et al. Vaccination with prefusion-stabilized respiratory syncytial virus fusion protein induces genetically and antigenically diverse antibody responses. Immunity. 2021;54:769-780.e6 pubmed publisher
Rajamanickam V, Ballesteros Merino C, Samson K, Ross D, Bernard B, Fox B, et al. Robust Antitumor Immunity in a Patient with Metastatic Colorectal Cancer Treated with Cytotoxic Regimens. Cancer Immunol Res. 2021;9:602-611 pubmed publisher
Townsley S, Donofrio G, Jian N, Leggat D, Dussupt V, Mendez Rivera L, et al. B cell engagement with HIV-1 founder virus envelope predicts development of broadly neutralizing antibodies. Cell Host Microbe. 2021;29:564-578.e9 pubmed publisher
Duhen R, Ballesteros Merino C, Frye A, Tran E, Rajamanickam V, Chang S, et al. Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells. Nat Commun. 2021;12:1047 pubmed publisher
Sutton H, Aye R, Idris A, Vistein R, Nduati E, Kai O, et al. Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans. Cell Rep. 2021;34:108684 pubmed publisher
Litchfield K, Reading J, Puttick C, Thakkar K, Abbosh C, Bentham R, et al. Meta-analysis of tumor- and T cell-intrinsic mechanisms of sensitization to checkpoint inhibition. Cell. 2021;184:596-614.e14 pubmed publisher
Eslamizar L, Petrovas C, Leggat D, Furr K, Lifton M, Levine G, et al. Recombinant MVA-prime elicits neutralizing antibody responses by inducing antigen-specific B cells in the germinal center. NPJ Vaccines. 2021;6:15 pubmed publisher
Yang R, Mele F, Worley L, Langlais D, Rosain J, Benhsaien I, et al. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. Cell. 2020;183:1826-1847.e31 pubmed publisher
Port J, Wozniak D, Oestereich L, Pallasch E, Becker Ziaja B, M xfc ller J, et al. Severe Human Lassa Fever Is Characterized by Nonspecific T-Cell Activation and Lymphocyte Homing to Inflamed Tissues. J Virol. 2020;94: pubmed publisher
Juno J, Tan H, Lee W, Reynaldi A, Kelly H, Wragg K, et al. Humoral and circulating follicular helper T cell responses in recovered patients with COVID-19. Nat Med. 2020;26:1428-1434 pubmed publisher
Bennstein S, Weinhold S, Manser A, Scherenschlich N, Noll A, Raba K, et al. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells. elife. 2020;9: pubmed publisher
Mothe B, Rosás Umbert M, Coll P, Manzardo C, Puertas M, Morón López S, et al. HIVconsv Vaccines and Romidepsin in Early-Treated HIV-1-Infected Individuals: Safety, Immunogenicity and Effect on the Viral Reservoir (Study BCN02). Front Immunol. 2020;11:823 pubmed publisher
Aliu B, Demeestere D, Seydoux E, Boucraut J, Delmont E, Brodovitch A, et al. Selective inhibition of anti-MAG IgM autoantibody binding to myelin by an antigen-specific glycopolymer. J Neurochem. 2020;154:486-501 pubmed publisher
Yang L, Xue X, Zeng T, Chen X, Zhao Q, Tang X, et al. Novel biallelic TRNT1 mutations lead to atypical SIFD and multiple immune defects. Genes Dis. 2020;7:128-137 pubmed publisher
Silver J, Humbles A, Ohne Y. Isolation, Culture, and Induction of Plasticity in ILC2s. Methods Mol Biol. 2020;2121:115-127 pubmed publisher
Haruna M, Hirata M, Iwahori K, Kanazawa T, Yamamoto Y, Goto K, et al. Docetaxel Upregulates HMGB1 Levels in Non-small Cell Lung Cancer. Biol Pharm Bull. 2020;43:399-403 pubmed publisher
Fong L, Hotson A, Powderly J, Sznol M, Heist R, Choueiri T, et al. Adenosine 2A Receptor Blockade as an Immunotherapy for Treatment-Refractory Renal Cell Cancer. Cancer Discov. 2020;10:40-53 pubmed publisher
Collinson Pautz M, Chang W, Lu A, Khalil M, Crisostomo J, Lin P, et al. Constitutively active MyD88/CD40 costimulation enhances expansion and efficacy of chimeric antigen receptor T cells targeting hematological malignancies. Leukemia. 2019;: pubmed publisher
Iwahori K, Shintani Y, Funaki S, Yamamoto Y, Matsumoto M, Yoshida T, et al. Peripheral T cell cytotoxicity predicts T cell function in the tumor microenvironment. Sci Rep. 2019;9:2636 pubmed publisher
Beraud C, Lemaire M, Perez Bercoff D. Reassessment of the capacity of the HIV-1 Env cytoplasmic domain to trigger NF-?B activation. Virol J. 2018;15:35 pubmed publisher
Carnevale G, Carpino G, Cardinale V, Pisciotta A, Riccio M, Bertoni L, et al. Activation of Fas/FasL pathway and the role of c-FLIP in primary culture of human cholangiocarcinoma cells. Sci Rep. 2017;7:14419 pubmed publisher
Moguche A, Musvosvi M, Penn Nicholson A, Plumlee C, Mearns H, Geldenhuys H, et al. Antigen Availability Shapes T Cell Differentiation and Function during Tuberculosis. Cell Host Microbe. 2017;21:695-706.e5 pubmed publisher
Telford W, Stickland L, Koschorreck M. Ultraviolet 320 nm laser excitation for flow cytometry. Cytometry A. 2017;91:314-325 pubmed publisher
Chandele A, Sewatanon J, Gunisetty S, Singla M, Onlamoon N, Akondy R, et al. Characterization of Human CD8 T Cell Responses in Dengue Virus-Infected Patients from India. J Virol. 2016;90:11259-11278 pubmed
Wheatley A, Kristensen A, Lay W, Kent S. HIV-dependent depletion of influenza-specific memory B cells impacts B cell responsiveness to seasonal influenza immunisation. Sci Rep. 2016;6:26478 pubmed publisher
Bruno L, Cortese M, Monda G, Gentile M, Calò S, Schiavetti F, et al. Human cytomegalovirus pUL10 interacts with leukocytes and impairs TCR-mediated T-cell activation. Immunol Cell Biol. 2016;94:849-860 pubmed publisher
Kariminia A, Holtan S, Ivison S, Rozmus J, Hébert M, Martin P, et al. Heterogeneity of chronic graft-versus-host disease biomarkers: association with CXCL10 and CXCR3+ NK cells. Blood. 2016;127:3082-91 pubmed publisher
Hammitzsch A, Tallant C, Fedorov O, O Mahony A, Brennan P, Hay D, et al. CBP30, a selective CBP/p300 bromodomain inhibitor, suppresses human Th17 responses. Proc Natl Acad Sci U S A. 2015;112:10768-73 pubmed publisher
Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD8alpha
Apps. Abbrev. :
FC
Cat # :
301048
Clone :
RPA-T8
Item :
Brilliant Violet 510™ anti-human CD8a
Isotype :
Mouse IgG1, κ
Other Names :
T8, Leu2
Size :
100 tests
Price (USD) :
380 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV510
Application Notes :
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections^3 and costimulation of T cell responses^4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA