product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 785™ anti-human CD8a
catalog :
301046
quantity :
100 tests
price :
396 USD
clonality :
monoclonal
host :
mouse
conjugate :
Brilliant Violet 785
clone name :
RPA-T8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 3f, 3g
Fransen N, Crusius J, Smolders J, Mizee M, Van Eden C, Luchetti S, et al. Post-mortem multiple sclerosis lesion pathology is influenced by single nucleotide polymorphisms. Brain Pathol. 2020;30:106-119 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Remmerswaal E, Hombrink P, Nota B, Pircher H, ten Berge I, van Lier R, et al. Expression of IL-7Rα and KLRG1 defines functionally distinct CD8+ T-cell populations in humans. Eur J Immunol. 2019;49:694-708 pubmed publisher
  • flow cytometry; human; loading ...; fig s4b
Galperin M, Farenc C, Mukhopadhyay M, Jayasinghe D, Decroos A, Benati D, et al. CD4+ T cell-mediated HLA class II cross-restriction in HIV controllers. Sci Immunol. 2018;3: pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Bissa M, Kim S, Galli V, Fourati S, Sarkis S, Arakelyan A, et al. HIV vaccine candidate efficacy in female macaques mediated by cAMP-dependent efferocytosis and V2-specific ADCC. Nat Commun. 2023;14:575 pubmed publisher
Gilman K, Cracchiolo M, Matiatos A, Davini D, Simpson R, Katsanis E. Partially replacing cyclophosphamide with bendamustine in combination with cyclosporine A improves survival and reduces xenogeneic graft-versus-host-disease. Front Immunol. 2022;13:1045710 pubmed publisher
Sugrue J, Posseme C, Tan Z, Pou C, Charbit B, Bondet V, et al. Enhanced TLR3 responsiveness in hepatitis C virus resistant women from the Irish anti-D cohort. Cell Rep Med. 2022;3:100804 pubmed publisher
Duan L, Cui X, Zhu K, Yao L, Wang G, Cao W, et al. SARS-CoV-2 vaccine-induced antibody and T cell response in SARS-CoV-1 survivors. Cell Rep. 2022;40:111284 pubmed publisher
van der Ploeg K, Kirosingh A, Mori D, Chakraborty S, Hu Z, Sievers B, et al. TNF-α+ CD4+ T cells dominate the SARS-CoV-2 specific T cell response in COVID-19 outpatients and are associated with durable antibodies. Cell Rep Med. 2022;3:100640 pubmed publisher
Pan R, Ryan J, Pan D, Wucherpfennig K, Letai A. Augmenting NK cell-based immunotherapy by targeting mitochondrial apoptosis. Cell. 2022;185:1521-1538.e18 pubmed publisher
Swadling L, Diniz M, Schmidt N, Amin O, Chandran A, Shaw E, et al. Pre-existing polymerase-specific T cells expand in abortive seronegative SARS-CoV-2. Nature. 2022;601:110-117 pubmed publisher
Kip P, Sluiter T, Moore J, Hart A, Ruske J, O LEARY J, et al. Short-Term Pre-Operative Protein Caloric Restriction in Elective Vascular Surgery Patients: A Randomized Clinical Trial. Nutrients. 2021;13: pubmed publisher
Hurov K, Lahdenranta J, Upadhyaya P, Haines E, Cohen H, Repash E, et al. BT7480, a novel fully synthetic Bicycle tumor-targeted immune cell agonist™ (Bicycle TICA™) induces tumor localized CD137 agonism. J Immunother Cancer. 2021;9: pubmed publisher
Guo X, Kazanova A, Thurmond S, Saragovi H, Rudd C. Effective chimeric antigen receptor T cells against SARS-CoV-2. iScience. 2021;24:103295 pubmed publisher
Farhat A, Weiner A, Posner C, Kim Z, Orcutt Jahns B, Carlson S, et al. Modeling cell-specific dynamics and regulation of the common gamma chain cytokines. Cell Rep. 2021;35:109044 pubmed publisher
Schreurs R, Sagebiel A, Steinert F, Highton A, Klarenbeek P, Drewniak A, et al. Intestinal CD8+ T cell responses are abundantly induced early in human development but show impaired cytotoxic effector capacities. Mucosal Immunol. 2021;14:605-614 pubmed publisher
Buggert M, Vella L, Nguyen S, Wu V, Chen Z, Sekine T, et al. The Identity of Human Tissue-Emigrant CD8+ T Cells. Cell. 2020;183:1946-1961.e15 pubmed publisher
Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed publisher
Festag J, Thelemann T, Schell M, Raith S, Michel S, Jaschinski F, et al. Preventing ATP Degradation by ASO-Mediated Knockdown of CD39 and CD73 Results in A2aR-Independent Rescue of T Cell Proliferation. Mol Ther Nucleic Acids. 2020;21:656-669 pubmed publisher
Stunnenberg M, van Pul L, Sprokholt J, van Dort K, Gringhuis S, Geijtenbeek T, et al. MAVS Genetic Variation Is Associated with Decreased HIV-1 Replication In Vitro and Reduced CD4+ T Cell Infection in HIV-1-Infected Individuals. Viruses. 2020;12: pubmed publisher
Aram J, Frakich N, Morandi E, Alrouji M, Samaraweera A, Onion D, et al. Increased IL-2 and Reduced TGF-β Upon T-Cell Stimulation are Associated with GM-CSF Upregulation in Multiple Immune Cell Types in Multiple Sclerosis. Biomedicines. 2020;8: pubmed publisher
Antonucci L, Porcu C, Timperi E, Santini S, Iannucci G, Balsano C. Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity. J Immunol Res. 2020;2020:4570219 pubmed publisher
Kumagai S, Togashi Y, Sakai C, Kawazoe A, Kawazu M, Ueno T, et al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells. Immunity. 2020;53:187-203.e8 pubmed publisher
Christiansen M, Offersen R, Jensen J, Petersen M, Larsen C, Mogensen T. Identification of Novel Genetic Variants in CVID Patients With Autoimmunity, Autoinflammation, or Malignancy. Front Immunol. 2019;10:3022 pubmed publisher
Swadling L, Pallett L, Diniz M, Baker J, Amin O, Stegmann K, et al. Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence. Cell Rep. 2020;30:687-698.e6 pubmed publisher
Hataye J, Casazza J, Best K, Liang C, Immonen T, Ambrozak D, et al. Principles Governing Establishment versus Collapse of HIV-1 Cellular Spread. Cell Host Microbe. 2019;26:748-763.e20 pubmed publisher
Huang B, Chen Z, Geng L, Wang J, Liang H, Cao Y, et al. Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways. Cell. 2019;179:1160-1176.e24 pubmed publisher
Schreurs R, Baumdick M, Sagebiel A, Kaufmann M, Mokry M, Klarenbeek P, et al. Human Fetal TNF-α-Cytokine-Producing CD4+ Effector Memory T Cells Promote Intestinal Development and Mediate Inflammation Early in Life. Immunity. 2019;50:462-476.e8 pubmed publisher
Querfeld C, Leung S, Myskowski P, Curran S, Goldman D, Heller G, et al. Primary T Cells from Cutaneous T-cell Lymphoma Skin Explants Display an Exhausted Immune Checkpoint Profile. Cancer Immunol Res. 2018;6:900-909 pubmed publisher
Claireaux M, Galperin M, Benati D, Nouel A, Mukhopadhyay M, Klingler J, et al. A High Frequency of HIV-Specific Circulating Follicular Helper T Cells Is Associated with Preserved Memory B Cell Responses in HIV Controllers. MBio. 2018;9: pubmed publisher
Carisey A, Mace E, Saeed M, Davis D, Orange J. Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells. Curr Biol. 2018;28:489-502.e9 pubmed publisher
Mukhopadhyay M, Galperin M, Patgaonkar M, Vasan S, Ho D, Nouël A, et al. DNA Vaccination by Electroporation Amplifies Broadly Cross-Restricted Public TCR Clonotypes Shared with HIV Controllers. J Immunol. 2017;199:3437-3452 pubmed publisher
Taraldsrud E, Fevang B, Jørgensen S, Moltu K, Hilden V, Taskén K, et al. Defective IL-4 signaling in T cells defines severe common variable immunodeficiency. J Autoimmun. 2017;81:110-119 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD8alpha
Apps. Abbrev. :
FC
Cat # :
301046
Clone :
RPA-T8
Item :
Brilliant Violet 785™ anti-human CD8a
Isotype :
Mouse IgG1, κ
Other Names :
T8, Leu2
Size :
100 tests
Price (USD) :
396 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV785
Application Notes :
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections^3 and costimulation of T cell responses^4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA