product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 570™ anti-human CD8a
catalog :
301037
quantity :
25 tests
price :
209 USD
clonality :
monoclonal
host :
mouse
conjugate :
Brilliant Violet 570
clone name :
RPA-T8
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1b
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 1d
Roberts E, Carnathan D, Li H, Shaw G, Silvestri G, Betts M. Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathog. 2016;12:e1006135 pubmed publisher
  • flow cytometry; human; fig s4a
Paquin Proulx D, Gibbs A, Bachle S, Checa A, Introini A, Leeansyah E, et al. Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion. J Immunol. 2016;197:1843-51 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...
Vaccari M, Gordon S, Fourati S, Schifanella L, Liyanage N, Cameron M, et al. Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition. Nat Med. 2016;22:762-70 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 8b
Zurawski G, Zurawski S, Flamar A, Richert L, Wagner R, Tomaras G, et al. Targeting HIV-1 Env gp140 to LOX-1 Elicits Immune Responses in Rhesus Macaques. PLoS ONE. 2016;11:e0153484 pubmed publisher
  • flow cytometry; rhesus macaque; fig 3
Bolton D, Pegu A, Wang K, McGinnis K, Nason M, Foulds K, et al. Human Immunodeficiency Virus Type 1 Monoclonal Antibodies Suppress Acute Simian-Human Immunodeficiency Virus Viremia and Limit Seeding of Cell-Associated Viral Reservoirs. J Virol. 2016;90:1321-32 pubmed publisher
  • flow cytometry; human; fig s1
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
  • flow cytometry; human
Ito S, Bollard C, Carlsten M, Melenhorst J, Biancotto A, Wang E, et al. Ultra-low dose interleukin-2 promotes immune-modulating function of regulatory T cells and natural killer cells in healthy volunteers. Mol Ther. 2014;22:1388-1395 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Mudd P, Minervina A, Pogorelyy M, Turner J, Kim W, Kalaidina E, et al. SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humans. Cell. 2022;185:603-613.e15 pubmed publisher
Romero Olmedo A, Schulz A, Hochstätter S, Das Gupta D, Virta I, Hirseland H, et al. Induction of robust cellular and humoral immunity against SARS-CoV-2 after a third dose of BNT162b2 vaccine in previously unresponsive older adults. Nat Microbiol. 2022;7:195-199 pubmed publisher
Tauzin A, Nayrac M, Benlarbi M, Gong S, Gasser R, Beaudoin Bussieres G, et al. A single dose of the SARS-CoV-2 vaccine BNT162b2 elicits Fc-mediated antibody effector functions and T cell responses. Cell Host Microbe. 2021;29:1137-1150.e6 pubmed publisher
Mold J, Modolo L, Hård J, Zamboni M, Larsson A, Stenudd M, et al. Divergent clonal differentiation trajectories establish CD8+ memory T cell heterogeneity during acute viral infections in humans. Cell Rep. 2021;35:109174 pubmed publisher
Maleki K, Tauriainen J, García M, Kerkman P, Christ W, Dias J, et al. MAIT cell activation is associated with disease severity markers in acute hantavirus infection. Cell Rep Med. 2021;2:100220 pubmed publisher
Buggert M, Vella L, Nguyen S, Wu V, Chen Z, Sekine T, et al. The Identity of Human Tissue-Emigrant CD8+ T Cells. Cell. 2020;183:1946-1961.e15 pubmed publisher
Luoma A, Suo S, Williams H, Sharova T, Sullivan K, Manos M, et al. Molecular Pathways of Colon Inflammation Induced by Cancer Immunotherapy. Cell. 2020;: pubmed publisher
Vierboom M, Chenine A, Darrah P, Vervenne R, Boot C, Hofman S, et al. Evaluation of heterologous prime-boost vaccination strategies using chimpanzee adenovirus and modified vaccinia virus for TB subunit vaccination in rhesus macaques. NPJ Vaccines. 2020;5:39 pubmed publisher
Blasi M, Wescott E, Baker E, Mildenberg B, Labranche C, Rountree W, et al. Therapeutic vaccination with IDLV-SIV-Gag results in durable viremia control in chronically SHIV-infected macaques. NPJ Vaccines. 2020;5:36 pubmed publisher
Bradley T, Kuraoka M, Yeh C, Tian M, Chen H, Cain D, et al. Immune checkpoint modulation enhances HIV-1 antibody induction. Nat Commun. 2020;11:948 pubmed publisher
Han Q, Bradley T, Williams W, Cain D, Montefiori D, Saunders K, et al. Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following HIV Envelope Immunization. Cell Rep. 2020;30:1553-1569.e6 pubmed publisher
de Andrade L, Lu Y, Luoma A, Ito Y, Pan D, Pyrdol J, et al. Discovery of specialized NK cell populations infiltrating human melanoma metastases. JCI Insight. 2019;4: pubmed publisher
Escudero Pérez B, Ruibal P, Rottstegge M, Lüdtke A, Port J, Hartmann K, et al. Comparative pathogenesis of Ebola virus and Reston virus infection in humanized mice. JCI Insight. 2019;4: pubmed publisher
Tran T, Guha R, Portugal S, Skinner J, Ongoiba A, Bhardwaj J, et al. A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation. Immunity. 2019;51:750-765.e10 pubmed publisher
Marquardt N, Kekäläinen E, Chen P, Lourda M, Wilson J, Scharenberg M, et al. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells. Nat Commun. 2019;10:3841 pubmed publisher
Darrah P, Difazio R, Maiello P, Gideon H, Myers A, Rodgers M, et al. Boosting BCG with proteins or rAd5 does not enhance protection against tuberculosis in rhesus macaques. NPJ Vaccines. 2019;4:21 pubmed publisher
Shifrut E, Carnevale J, Tobin V, Roth T, Woo J, Bui C, et al. Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Cell. 2018;175:1958-1971.e15 pubmed publisher
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
Kang H, Subramaniam M, Targ S, Nguyen M, Maliskova L, McCarthy E, et al. Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. Nat Biotechnol. 2017;: pubmed publisher
Nishimura Y, Gautam R, Chun T, Sadjadpour R, Foulds K, Shingai M, et al. Early antibody therapy can induce long-lasting immunity to SHIV. Nature. 2017;543:559-563 pubmed publisher
Negri D, Blasi M, Labranche C, Parks R, Balachandran H, Lifton M, et al. Immunization with an SIV-based IDLV Expressing HIV-1 Env 1086 Clade C Elicits Durable Humoral and Cellular Responses in Rhesus Macaques. Mol Ther. 2016;24:2021-2032 pubmed publisher
Hensley McBain T, Zevin A, Manuzak J, Smith E, Gile J, Miller C, et al. Effects of Fecal Microbial Transplantation on Microbiome and Immunity in Simian Immunodeficiency Virus-Infected Macaques. J Virol. 2016;90:4981-4989 pubmed publisher
Swanstrom A, Haggarty B, Jordan A, Romano J, Leslie G, Aye P, et al. Derivation and Characterization of a CD4-Independent, Non-CD4-Tropic Simian Immunodeficiency Virus. J Virol. 2016;90:4966-4980 pubmed publisher
García Arriaza J, Perdiguero B, Heeney J, Seaman M, Montefiori D, Labranche C, et al. Head-to-Head Comparison of Poxvirus NYVAC and ALVAC Vectors Expressing Identical HIV-1 Clade C Immunogens in Prime-Boost Combination with Env Protein in Nonhuman Primates. J Virol. 2015;89:8525-39 pubmed publisher
Mawa P, Nkurunungi G, Egesa M, Webb E, Smith S, Kizindo R, et al. The impact of maternal infection with Mycobacterium tuberculosis on the infant response to bacille Calmette-Guérin immunization. Philos Trans R Soc Lond B Biol Sci. 2015;370: pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD8alpha
Apps. Abbrev. :
FC
Cat # :
301037
Clone :
RPA-T8
Item :
Brilliant Violet 570™ anti-human CD8a
Isotype :
Mouse IgG1, κ
Other Names :
T8, Leu2
Size :
25 tests
Price (USD) :
209 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV570
Application Notes :
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections^3 and costimulation of T cell responses^4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA