product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-human CD8a
catalog :
301035
quantity :
25 tests
price :
190 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV421
clone name :
RPA-T8
reactivity :
human, mouse, rhesus macaque
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:1000; loading ...
Nagle V, Henry K, Hertz C, Graham M, Campos C, Parada L, et al. Imaging Tumor-Infiltrating Lymphocytes in Brain Tumors with [64Cu]Cu-NOTA-anti-CD8 PET. Clin Cancer Res. 2021;27:1958-1966 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Lin Y, Schmidt M, Sharif G, Vietsch E, Kiliti A, Barefoot M, et al. Impaired CXCL12 signaling contributes to resistance of pancreatic cancer subpopulations to T cell-mediated cytotoxicity. Oncoimmunology. 2022;11:2027136 pubmed publisher
Kim M, Cooper M, Jacobs M, Ritchey J, Hollaway J, Fehniger T, et al. CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy. JCI Insight. 2021;6: pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Koutsakos M, Rowntree L, Hensen L, Chua B, van de Sandt C, Habel J, et al. Integrated immune dynamics define correlates of COVID-19 severity and antibody responses. Cell Rep Med. 2021;2:100208 pubmed publisher
Bartok O, Pataskar A, Nagel R, Laos M, Goldfarb E, Hayoun D, et al. Anti-tumour immunity induces aberrant peptide presentation in melanoma. Nature. 2021;590:332-337 pubmed publisher
Li N, Tang N, Cheng C, Hu T, Wei X, Han W, et al. Improving the anti-solid tumor efficacy of CAR-T cells by inhibiting adenosine signaling pathway. Oncoimmunology. 2020;9:1824643 pubmed publisher
Cheng C, Duan H, Xu K, Chuang G, Corrigan A, Geng H, et al. Immune Monitoring Reveals Fusion Peptide Priming to Imprint Cross-Clade HIV-Neutralizing Responses with a Characteristic Early B Cell Signature. Cell Rep. 2020;32:107981 pubmed publisher
Di Genua C, Valletta S, Buono M, Stoilova B, Sweeney C, Rodriguez Meira A, et al. C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor. Cancer Cell. 2020;37:690-704.e8 pubmed publisher
Kim D, Kobayashi T, Voisin B, Jo J, Sakamoto K, Jin S, et al. Targeted therapy guided by single-cell transcriptomic analysis in drug-induced hypersensitivity syndrome: a case report. Nat Med. 2020;26:236-243 pubmed publisher
Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, et al. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization. Cell. 2019;178:567-584.e19 pubmed publisher
Nüssing S, Koay H, Sant S, Loudovaris T, Mannering S, Lappas M, et al. Divergent SATB1 expression across human life span and tissue compartments. Immunol Cell Biol. 2019;97:498-511 pubmed publisher
Rousset F, Salmon P, Bredl S, Cherpin O, Coelho M, Myburgh R, et al. Optimizing Synthetic miRNA Minigene Architecture for Efficient miRNA Hairpin Concatenation and Multi-target Gene Knockdown. Mol Ther Nucleic Acids. 2018;14:351-363 pubmed publisher
Rochat M, Schlaepfer E, Kuster S, Li D, Audigé A, Ivic S, et al. Monitoring HIV DNA and cellular activation markers in HIV-infected humanized mice under cART. Virol J. 2018;15:191 pubmed publisher
Brozy J, Schlaepfer E, Mueller C, Rochat M, Rampini S, Myburgh R, et al. Antiviral Activity of HIV gp120-Targeting Bispecific T Cell Engager Antibody Constructs. J Virol. 2018;92: pubmed publisher
Lettau M, Armbrust F, Dohmen K, Drews L, Poch T, Dietz M, et al. Mechanistic peculiarities of activation-induced mobilization of cytotoxic effector proteins in human T cells. Int Immunol. 2018;30:215-228 pubmed publisher
Martin S, Wick D, Nielsen J, Little N, Holt R, Nelson B. A library-based screening method identifies neoantigen-reactive T cells in peripheral blood prior to relapse of ovarian cancer. Oncoimmunology. 2017;7:e1371895 pubmed publisher
Audigé A, Rochat M, Li D, Ivic S, Fahrny A, Muller C, et al. Long-term leukocyte reconstitution in NSG mice transplanted with human cord blood hematopoietic stem and progenitor cells. BMC Immunol. 2017;18:28 pubmed publisher
Martins M, Tully D, Cruz M, Power K, Veloso de Santana M, Bean D, et al. Vaccine-Induced Simian Immunodeficiency Virus-Specific CD8+ T-Cell Responses Focused on a Single Nef Epitope Select for Escape Variants Shortly after Infection. J Virol. 2015;89:10802-20 pubmed publisher
Sanders K, Fox B, Bzik D. Attenuated Toxoplasma gondii Stimulates Immunity to Pancreatic Cancer by Manipulation of Myeloid Cell Populations. Cancer Immunol Res. 2015;3:891-901 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD8alpha
Apps. Abbrev. :
FC, ICC
Cat # :
301035
Clone :
RPA-T8
Item :
Brilliant Violet 421™ anti-human CD8a
Isotype :
Mouse IgG1, κ
Other Names :
T8, Leu2
Size :
25 tests
Price (USD) :
190 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV421
Application Notes :
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections^3 and costimulation of T cell responses^4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA