Pacific Blue™ anti-human CD8a | BioLegend 301026 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Pacific Blue™ anti-human CD8a

catalog :

301026

quantity :

25 μg

price :

105 USD

clonality :

monoclonal

host :

mouse

conjugate :

Pacific Blue

clone name :

RPA-T8

reactivity :

human, rhesus macaque

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 10 ug/ml	Khazen R, MÃ¼ller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S. Melanoma cell lysosome secretory burst neutralizes the CTL-mediated cytotoxicity at the lytic synapse. Nat Commun. 2016;7:10823 pubmed publisher
flow cytometry; human	Misra R, Shah S, Fowell D, Wang H, Scheible K, Misra S, et al. Preterm cord blood CD4âº T cells exhibit increased IL-6 production in chorioamnionitis and decreased CD4âº T cells in bronchopulmonary dysplasia. Hum Immunol. 2015;76:329-338 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Corgnac S, Lecluse Y, Mami Chouaib F. Isolation of tumor-resident CD8+ T cells from human lung tumors. STAR Protoc. 2021;2:100267 pubmed publisher
	Yang R, Mele F, Worley L, Langlais D, Rosain J, Benhsaien I, et al. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. Cell. 2020;183:1826-1847.e31 pubmed publisher
	Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
	Bäcker Koduah P, Infante Duarte C, Ivaldi F, Uccelli A, Bellmann Strobl J, Wernecke K, et al. Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS. Ann Clin Transl Neurol. 2020;: pubmed publisher
	Stunnenberg M, van Pul L, Sprokholt J, van Dort K, Gringhuis S, Geijtenbeek T, et al. MAVS Genetic Variation Is Associated with Decreased HIV-1 Replication In Vitro and Reduced CD4+ T Cell Infection in HIV-1-Infected Individuals. Viruses. 2020;12: pubmed publisher
	Leclerc M, Voilin E, Gros G, Corgnac S, de Montpreville V, Validire P, et al. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1. Nat Commun. 2019;10:3345 pubmed publisher
	Durgeau A, Virk Y, Gros G, Voilin E, Corgnac S, Djenidi F, et al. Human preprocalcitonin self-antigen generates TAP-dependent and -independent epitopes triggering optimised T-cell responses toward immune-escaped tumours. Nat Commun. 2018;9:5097 pubmed publisher
	Toepfner N, Herold C, Otto O, Rosendahl P, Jacobi A, Kr ter M, et al. Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood. elife. 2018;7: pubmed publisher
	Wu H, Wiseman R, Hughes C, Webb G, Abdulhaqq S, Bimber B, et al. The Role of MHC-E in T Cell Immunity Is Conserved among Humans, Rhesus Macaques, and Cynomolgus Macaques. J Immunol. 2018;200:49-60 pubmed publisher
	Koreth J, Kim H, Jones K, Lange P, Reynolds C, Chammas M, et al. Efficacy, durability, and response predictors of low-dose interleukin-2 therapy for chronic graft-versus-host disease. Blood. 2016;128:130-7 pubmed publisher
	Serr I, FÃ¼rst R, Achenbach P, Scherm M, GÃ¶kmen F, Haupt F, et al. Type 1 diabetes vaccine candidates promote human Foxp3(+)Treg induction in humanized mice. Nat Commun. 2016;7:10991 pubmed publisher
	Petit N, Lambert Niclot S, Marcelin A, Garcia S, Marodon G. HIV Replication Is Not Controlled by CD8+ T Cells during the Acute Phase of the Infection in Humanized Mice. PLoS ONE. 2015;10:e0138420 pubmed publisher
	Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
	Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed

product information

Antigen :

CD8a

Apps. Abbrev. :

Flow Cytometry

Cat # :

301026

Clone :

RPA-T8

Item :

Pacific Blue™ anti-human CD8a

Isotype :

Mouse IgG1, κ

Other Names :

T8, Leu2

Size :

25 μg

Price (USD) :

105 USD

Reactivity :

Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Application Notes :

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections 3 and costimulation of T cell responses 4 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. Nos. 301073 301074).

more info or order :

BioLegend product webpage