product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
TotalSeq™-C0072 anti-human CD4
catalog :
300567
quantity :
10 μg
price :
369 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RPA-T4
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 62
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1a
Horiuchi S, Wu H, Liu W, Schmitt N, Provot J, Liu Y, et al. Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells. Sci Adv. 2021;7:eabj1249 pubmed publisher
  • immunohistochemistry; human; fig 1a
Ming S, Yin H, Li X, Gong S, Zhang G, Wu Y. GITR Promotes the Polarization of TFH-Like Cells in Helicobacter pylori-Positive Gastritis. Front Immunol. 2021;12:736269 pubmed publisher
  • flow cytometry; human; 5:100; loading ...; fig 3e
Yamagishi M, Kubokawa M, Kuze Y, Suzuki A, Yokomizo A, Kobayashi S, et al. Chronological genome and single-cell transcriptome integration characterizes the evolutionary process of adult T cell leukemia-lymphoma. Nat Commun. 2021;12:4821 pubmed publisher
  • flow cytometry; mouse; fig 2c
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
  • immunohistochemistry; human; 1:50; loading ...; fig 4d
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • flow cytometry; human; 1:400; loading ...; fig 1b
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; human; 3:50; loading ...; fig 3j
Park J, Botting R, Domínguez Conde C, Popescu D, Lavaert M, Kunz D, et al. A cell atlas of human thymic development defines T cell repertoire formation. Science. 2020;367: pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Park C, Kehrl J. An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph node. elife. 2019;8: pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Pecher A, Kettemann F, Asteriti E, Schmid H, Duerr Stoerzer S, Keppeler H, et al. Invariant natural killer T cells are functionally impaired in patients with systemic sclerosis. Arthritis Res Ther. 2019;21:212 pubmed publisher
  • flow cytometry; human; loading ...; fig s6c
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 7d, 7f
  • flow cytometry; human; loading ...; fig 7a, 7c, 7e
Rasoulouniriana D, Santana Magal N, Gutwillig A, Farhat Younis L, Wine Y, Saperia C, et al. A distinct subset of FcγRI-expressing Th1 cells exert antibody-mediated cytotoxic activity. J Clin Invest. 2019;129:4151-4164 pubmed publisher
  • mass cytometry; human; loading ...
Banga R, Rebecchini C, Procopio F, Noto A, Munoz O, Ioannidou K, et al. Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement. PLoS Pathog. 2019;15:e1007918 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6d
Papaioannou E, Yanez D, Ross S, Lau C, Solanki A, Chawda M, et al. Sonic Hedgehog signaling limits atopic dermatitis via Gli2-driven immune regulation. J Clin Invest. 2019;129:3153-3170 pubmed publisher
  • flow cytometry; human; loading ...; fig 3f, 3g
Fransen N, Crusius J, Smolders J, Mizee M, Van Eden C, Luchetti S, et al. Post-mortem multiple sclerosis lesion pathology is influenced by single nucleotide polymorphisms. Brain Pathol. 2020;30:106-119 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Wen Z, Jin K, Shen Y, Yang Z, Li Y, Wu B, et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation. Nat Immunol. 2019;20:313-325 pubmed publisher
  • flow cytometry; human; fig 2a
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Tai Y, Lin L, Xing L, Cho S, Yu T, Acharya C, et al. APRIL signaling via TACI mediates immunosuppression by T regulatory cells in multiple myeloma: therapeutic implications. Leukemia. 2019;33:426-438 pubmed publisher
Sun M, Cao Y, Okada R, Reyes Gonz xe1 lez J, Stack H, Qin H, et al. Preclinical optimization of a GPC2-targeting CAR T-cell therapy for neuroblastoma. J Immunother Cancer. 2023;11: pubmed publisher
Xu C, Yang J, Kosters A, Babcock B, Qiu P, Ghosn E. Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system. iScience. 2022;25:105123 pubmed publisher
Gao Y, Cai C, Wullimann D, Niessl J, Rivera Ballesteros O, Chen P, et al. Immunodeficiency syndromes differentially impact the functional profile of SARS-CoV-2-specific T cells elicited by mRNA vaccination. Immunity. 2022;55:1732-1746.e5 pubmed publisher
Li S, Hickey A, Shangguan S, Ehrenberg P, Geretz A, Butler L, et al. HLA-B∗46 associates with rapid HIV disease progression in Asian cohorts and prominent differences in NK cell phenotype. Cell Host Microbe. 2022;30:1173-1185.e8 pubmed publisher
Lau D, Khare S, Stein M, Jain P, Gao Y, BenTaieb A, et al. Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer. Nat Commun. 2022;13:4053 pubmed publisher
Sureshchandra S, Zulu M, Doratt B, Jankeel A, Tifrea D, Edwards R, et al. Single-cell RNA sequencing reveals immunological rewiring at the maternal-fetal interface following asymptomatic/mild SARS-CoV-2 infection. Cell Rep. 2022;39:110938 pubmed publisher
Collora J, Liu R, Pinto Santini D, Ravindra N, Ganoza C, Lama J, et al. Single-cell multiomics reveals persistence of HIV-1 in expanded cytotoxic T cell clones. Immunity. 2022;55:1013-1031.e7 pubmed publisher
Bachireddy P, Azizi E, Burdziak C, Nguyen V, Ennis C, Maurer K, et al. Mapping the evolution of T cell states during response and resistance to adoptive cellular therapy. Cell Rep. 2021;37:109992 pubmed publisher
Shangguan S, Ehrenberg P, Geretz A, Yum L, Kundu G, May K, et al. Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1. elife. 2021;10: pubmed publisher
Beziat V, Rapaport F, Hu J, Titeux M, Bonnet des Claustres M, Bourgey M, et al. Humans with inherited T cell CD28 deficiency are susceptible to skin papillomaviruses but are otherwise healthy. Cell. 2021;184:3812-3828.e30 pubmed publisher
Hiltensperger M, Beltrán E, Kant R, Tyystjärvi S, Lepennetier G, Domínguez Moreno H, et al. Skin and gut imprinted helper T cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity. Nat Immunol. 2021;22:880-892 pubmed publisher
Chen L, Zhang Y, Pacheco K, Dai S. The T Cell Repertoires from Nickel Sensitized Joint Implant Failure Patients. Int J Mol Sci. 2021;22: pubmed publisher
Mohme M, Maire C, Schliffke S, Joosse S, Alawi M, Matschke J, et al. Molecular profiling of an osseous metastasis in glioblastoma during checkpoint inhibition: potential mechanisms of immune escape. Acta Neuropathol Commun. 2020;8:28 pubmed publisher
Canete P, Sweet R, Gonzalez Figueroa P, Papa I, Ohkura N, Bolton H, et al. Regulatory roles of IL-10-producing human follicular T cells. J Exp Med. 2019;: pubmed publisher
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
Boscheinen J, Thomann S, Knipe D, Deluca N, Schuler Thurner B, Gross S, et al. Generation of an Oncolytic Herpes Simplex Virus 1 Expressing Human MelanA. Front Immunol. 2019;10:2 pubmed publisher
Bruner K, Wang Z, Simonetti F, Bender A, Kwon K, Sengupta S, et al. A quantitative approach for measuring the reservoir of latent HIV-1 proviruses. Nature. 2019;566:120-125 pubmed publisher
Einkauf K, Lee G, Gao C, Sharaf R, Sun X, Hua S, et al. Intact HIV-1 proviruses accumulate at distinct chromosomal positions during prolonged antiretroviral therapy. J Clin Invest. 2019;129:988-998 pubmed publisher
Rosental B, Kowarsky M, Seita J, Corey D, Ishizuka K, Palmeri K, et al. Complex mammalian-like haematopoietic system found in a colonial chordate. Nature. 2018;564:425-429 pubmed publisher
van Montfoort N, Borst L, Korrer M, Sluijter M, Marijt K, Santegoets S, et al. NKG2A Blockade Potentiates CD8 T Cell Immunity Induced by Cancer Vaccines. Cell. 2018;175:1744-1755.e15 pubmed publisher
Yoshitomi H, Kobayashi S, Miyagawa Hayashino A, Okahata A, Doi K, Nishitani K, et al. Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat Commun. 2018;9:3762 pubmed publisher
Pissani F, Schulte B, Eller M, Schultz B, Ratto Kim S, Marovich M, et al. Modulation of Vaccine-Induced CD4 T Cell Functional Profiles by Changes in Components of HIV Vaccine Regimens in Humans. J Virol. 2018;92: pubmed publisher
Yang J, Wang Z, Cai H, Yuan L, Hu M, Wu M, et al. Sex Differences in Neuropathology and Cognitive Behavior in APP/PS1/tau Triple-Transgenic Mouse Model of Alzheimer's Disease. Neurosci Bull. 2018;34:736-746 pubmed publisher
Achita P, Dervović D, Ly D, Lee J, Haug T, Joe B, et al. Infusion of ex-vivo expanded human TCR-αβ+ double-negative regulatory T cells delays onset of xenogeneic graft-versus-host disease. Clin Exp Immunol. 2018;193:386-399 pubmed publisher
Yang T, St John L, Garber H, Kerros C, Ruisaard K, Clise Dwyer K, et al. Membrane-Associated Proteinase 3 on Granulocytes and Acute Myeloid Leukemia Inhibits T Cell Proliferation. J Immunol. 2018;201:1389-1399 pubmed publisher
Noto A, Procopio F, Banga R, Suffiotti M, Corpataux J, Cavassini M, et al. CD32+ and PD-1+ Lymph Node CD4 T Cells Support Persistent HIV-1 Transcription in Treated Aviremic Individuals. J Virol. 2018;92: pubmed publisher
Romani R, Manni G, Donati C, Pirisinu I, Bernacchioni C, Gargaro M, et al. S1P promotes migration, differentiation and immune regulatory activity in amniotic-fluid-derived stem cells. Eur J Pharmacol. 2018;833:173-182 pubmed publisher
Kiener R, Fleischmann M, Wiegand M, Lemmermann N, Schwegler C, Kaufmann C, et al. Efficient Delivery of Human Cytomegalovirus T Cell Antigens by Attenuated Sendai Virus Vectors. J Virol. 2018;92: pubmed publisher
Vadrevu S, Trbojević Akmačić I, Kossenkov A, Colomb F, Giron L, Anzurez A, et al. Frontline Science: Plasma and immunoglobulin G galactosylation associate with HIV persistence during antiretroviral therapy. J Leukoc Biol. 2018;104:461-471 pubmed publisher
Ostrov D, Alkanani A, McDaniel K, Case S, Baschal E, Pyle L, et al. Methyldopa blocks MHC class II binding to disease-specific antigens in autoimmune diabetes. J Clin Invest. 2018;128:1888-1902 pubmed publisher
Dillen C, Pinsker B, Marusina A, Merleev A, Farber O, Liu H, et al. Clonally expanded γδ T cells protect against Staphylococcus aureus skin reinfection. J Clin Invest. 2018;128:1026-1042 pubmed publisher
Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
Sato K, Misawa N, Takeuchi J, Kobayashi T, Izumi T, Aso H, et al. Experimental Adaptive Evolution of Simian Immunodeficiency Virus SIVcpz to Pandemic Human Immunodeficiency Virus Type 1 by Using a Humanized Mouse Model. J Virol. 2018;92: pubmed publisher
Gaidt M, Ebert T, Chauhan D, Ramshorn K, Pinci F, Zuber S, et al. The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3. Cell. 2017;171:1110-1124.e18 pubmed publisher
Bogaert D, Kuehn H, Bonroy C, Calvo K, Dehoorne J, Vanlander A, et al. A novel IKAROS haploinsufficiency kindred with unexpectedly late and variable B-cell maturation defects. J Allergy Clin Immunol. 2018;141:432-435.e7 pubmed publisher
Teigler J, Zelinskyy G, Eller M, Bolton D, Marovich M, Gordon A, et al. Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection. J Virol. 2017;91: pubmed publisher
Schwerd T, Twigg S, Aschenbrenner D, Manrique S, Miller K, Taylor I, et al. A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis. J Exp Med. 2017;214:2547-2562 pubmed publisher
Hensel M, Peng T, Cheng A, De Rosa S, Wald A, Laing K, et al. Selective Expression of CCR10 and CXCR3 by Circulating Human Herpes Simplex Virus-Specific CD8 T Cells. J Virol. 2017;91: pubmed publisher
van den Broek T, Madi A, Delemarre E, Schadenberg A, Tesselaar K, Borghans J, et al. Human neonatal thymectomy induces altered B-cell responses and autoreactivity. Eur J Immunol. 2017;47:1970-1981 pubmed publisher
Miller S, Miles B, Guo K, Folkvord J, Meditz A, McCarter M, et al. Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1. J Virol. 2017;91: pubmed publisher
Costa N, Marques O, Godinho S, Carvalho C, Leal B, Figueiredo A, et al. Two separate effects contribute to regulatory T cell defect in systemic lupus erythematosus patients and their unaffected relatives. Clin Exp Immunol. 2017;189:318-330 pubmed publisher
Wahl S, Drong A, Lehne B, Loh M, Scott W, Kunze S, et al. Epigenome-wide association study of body mass index, and the adverse outcomes of adiposity. Nature. 2017;541:81-86 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
PG
Cat # :
300567
Clone :
RPA-T4
Item :
TotalSeq™-C0072 anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
10 μg
Price (USD) :
369 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
TOTALSEQ_C
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^3,4,5, blocking of T cell activation^1,2, and spatial biology (IBEX)^10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA