product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human CD4
catalog :
300537
quantity :
500 tests
price :
286 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
RPA-T4
reactivity :
human
application :
flow cytometry
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1
Ondigo B, Ndombi E, Nicholson S, Oguso J, Carter J, Kittur N, et al. Functional Studies of T Regulatory Lymphocytes in Human Schistosomiasis in Western Kenya. Am J Trop Med Hyg. 2018;98:1770-1781 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Koh W, Zayats R, López P, Murooka T. Visualizing Cellular Dynamics and Protein Localization in 3D Collagen. STAR Protoc. 2020;1:100203 pubmed publisher
Gludish D, Boliar S, Caldwell S, Tembo D, Chimbayo E, Jambo K, et al. TZM-gfp cells: a tractable fluorescent tool for analysis of rare and early HIV-1 infection. Sci Rep. 2020;10:19900 pubmed publisher
Dyson M, Masters E, Pazeraitis D, Perera R, Syrjänen J, Surade S, et al. Beyond affinity: selection of antibody variants with optimal biophysical properties and reduced immunogenicity from mammalian display libraries. MAbs. 2020;12:1829335 pubmed publisher
Penkava F, Velasco Herrera M, Young M, Yager N, Nwosu L, Pratt A, et al. Single-cell sequencing reveals clonal expansions of pro-inflammatory synovial CD8 T cells expressing tissue-homing receptors in psoriatic arthritis. Nat Commun. 2020;11:4767 pubmed publisher
Huber J, Chang Y, Meinl I, Kümpfel T, Meinl E, Baumjohann D. Fingolimod Profoundly Reduces Frequencies and Alters Subset Composition of Circulating T Follicular Helper Cells in Multiple Sclerosis Patients. J Immunol. 2020;204:1101-1110 pubmed publisher
Kim D, Kobayashi T, Voisin B, Jo J, Sakamoto K, Jin S, et al. Targeted therapy guided by single-cell transcriptomic analysis in drug-induced hypersensitivity syndrome: a case report. Nat Med. 2020;26:236-243 pubmed publisher
Carpenter R, Jiang R, Brennan F, Hall J, Gottipati M, Niewiesk S, et al. Human immune cells infiltrate the spinal cord and impair recovery after spinal cord injury in humanized mice. Sci Rep. 2019;9:19105 pubmed publisher
Tang M, Cheng L, Li F, Wu B, Chen P, Zhan Y, et al. Transcription Factor IRF4 Dysfunction Affects the Immunosuppressive Function of Treg Cells in Patients with Primary Immune Thrombocytopenia. Biomed Res Int. 2019;2019:1050285 pubmed publisher
Cui J, Zhang Q, Song Q, Wang H, Dmitriev P, Sun M, et al. Targeting hypoxia downstream signaling protein, CAIX, for CAR T-cell therapy against glioblastoma. Neuro Oncol. 2019;21:1436-1446 pubmed publisher
Kacherovsky N, Cardle I, Cheng E, Yu J, Baldwin M, Salipante S, et al. Traceless aptamer-mediated isolation of CD8+ T cells for chimeric antigen receptor T-cell therapy. Nat Biomed Eng. 2019;: pubmed publisher
Stuart T, Butler A, Hoffman P, Hafemeister C, Papalexi E, Mauck W, et al. Comprehensive Integration of Single-Cell Data. Cell. 2019;: pubmed publisher
Park M, Moon S, Baek J, Lee E, Jung K, Kim E, et al. Metformin Augments Anti-Inflammatory and Chondroprotective Properties of Mesenchymal Stem Cells in Experimental Osteoarthritis. J Immunol. 2019;203:127-136 pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
Souriant S, Balboa L, Dupont M, Pingris K, Kviatcovsky D, Cougoule C, et al. Tuberculosis Exacerbates HIV-1 Infection through IL-10/STAT3-Dependent Tunneling Nanotube Formation in Macrophages. Cell Rep. 2019;26:3586-3599.e7 pubmed publisher
Li B, SONG T, Wang F, Yin C, Li Z, Lin J, et al. Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion. Oncogenesis. 2019;8:17 pubmed publisher
Lundy S, Nikoopour E, Karoukis A, Ohara R, Othman M, Tagett R, et al. T Helper 1 Cellular Immunity Toward Recoverin Is Enhanced in Patients With Active Autoimmune Retinopathy. Front Med (Lausanne). 2018;5:249 pubmed publisher
Tang Huau T, Guéguen P, Goudot C, Durand M, Bohec M, Baulande S, et al. Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway. Nat Commun. 2018;9:2570 pubmed publisher
Wang F, Li B, Wei Y, Zhao Y, Wang L, Zhang P, et al. Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression. Oncogenesis. 2018;7:41 pubmed publisher
Martin G, Pace M, Thornhill J, Phetsouphanh C, Meyerowitz J, Gossez M, et al. CD32-Expressing CD4 T Cells Are Phenotypically Diverse and Can Contain Proviral HIV DNA. Front Immunol. 2018;9:928 pubmed publisher
Idorn M, Olsen M, Halldórsdóttir H, Skadborg S, Pedersen M, Høgdall C, et al. Improved migration of tumor ascites lymphocytes to ovarian cancer microenvironment by CXCR2 transduction. Oncoimmunology. 2018;7:e1412029 pubmed publisher
Yamada E, Nakaoka S, Klein L, Reith E, Langer S, Hopfensperger K, et al. Human-Specific Adaptations in Vpu Conferring Anti-tetherin Activity Are Critical for Efficient Early HIV-1 Replication In Vivo. Cell Host Microbe. 2018;23:110-120.e7 pubmed publisher
Hayatsu N, Miyao T, Tachibana M, Murakami R, Kimura A, Kato T, et al. Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells. Immunity. 2017;47:268-283.e9 pubmed publisher
Nakano Y, Misawa N, Juarez Fernandez G, Moriwaki M, Nakaoka S, Funo T, et al. HIV-1 competition experiments in humanized mice show that APOBEC3H imposes selective pressure and promotes virus adaptation. PLoS Pathog. 2017;13:e1006348 pubmed publisher
Iwata A, Durai V, Tussiwand R, Briseño C, Wu X, Grajales Reyes G, et al. Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF-IRF4 transcription factor complex. Nat Immunol. 2017;18:563-572 pubmed publisher
Kerstein A, Schüler S, Cabral Marques O, Fazio J, Häsler R, Muller A, et al. Environmental factor and inflammation-driven alteration of the total peripheral T-cell compartment in granulomatosis with polyangiitis. J Autoimmun. 2017;78:79-91 pubmed publisher
Beyer A, Muench M. Comparison of Human Hematopoietic Reconstitution in Different Strains of Immunodeficient Mice. Stem Cells Dev. 2017;26:102-112 pubmed publisher
Usero L, Sanchez A, Pizarro E, Xufré C, Marti M, Jaraquemada D, et al. Interleukin-13 Pathway Alterations Impair Invariant Natural Killer T-Cell-Mediated Regulation of Effector T Cells in Type 1 Diabetes. Diabetes. 2016;65:2356-66 pubmed publisher
Fletcher H, Snowden M, Landry B, Rida W, Satti I, Harris S, et al. T-cell activation is an immune correlate of risk in BCG vaccinated infants. Nat Commun. 2016;7:11290 pubmed publisher
Reichwald K, Jørgensen T, Skov S. TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells. PLoS ONE. 2014;9:e105627 pubmed publisher
Koelsch K, Wang Y, Maier Moore J, Sawalha A, Wren J. GFP affects human T cell activation and cytokine production following in vitro stimulation. PLoS ONE. 2013;8:e50068 pubmed publisher
André M, Erbacher A, Gille C, Schmauke V, Goecke B, Hohberger A, et al. Long-term human CD34+ stem cell-engrafted nonobese diabetic/SCID/IL-2R gamma(null) mice show impaired CD8+ T cell maintenance and a functional arrest of immature NK cells. J Immunol. 2010;185:2710-20 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
300537
Clone :
RPA-T4
Item :
APC anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
500 tests
Price (USD) :
286 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , blocking of T cell activation 1,2 , and spatial biology (IBEX) 10,11 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA