Brilliant Violet 421™ anti-human CD4 | BioLegend 300532 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human CD4

catalog :

300532

quantity :

100 tests

price :

345 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV421

clone name :

RPA-T4

reactivity :

human, mouse, chimpanzee

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1a	Tai Y, Lin L, Xing L, Cho S, Yu T, Acharya C, et al. APRIL signaling via TACI mediates immunosuppression by T regulatory cells in multiple myeloma: therapeutic implications. Leukemia. 2019;33:426-438 pubmed publisher
flow cytometry; mouse; 1:60; loading ...; fig 7a	Kiener R, Fleischmann M, Wiegand M, Lemmermann N, Schwegler C, Kaufmann C, et al. Efficient Delivery of Human Cytomegalovirus T Cell Antigens by Attenuated Sendai Virus Vectors. J Virol. 2018;92: pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Vanuytsel K, Villacorta Martin C, Lindstrom Vautrin J, Wang Z, Garcia Beltran W, Vrbanac V, et al. Multi-modal profiling of human fetal liver hematopoietic stem cells reveals the molecular signature of engraftment. Nat Commun. 2022;13:1103 pubmed publisher
	Chen Y, Feng R, He B, Wang J, Xian N, Huang G, et al. PD-1H Expression Associated With CD68 Macrophage Marker Confers an Immune-Activated Microenvironment and Favorable Overall Survival in Human Esophageal Squamous Cell Carcinoma. Front Mol Biosci. 2021;8:777370 pubmed publisher
	Sam J, Colombetti S, Fauti T, Roller A, Biehl M, Fahrni L, et al. Combination of T-Cell Bispecific Antibodies With PD-L1 Checkpoint Inhibition Elicits Superior Anti-Tumor Activity. Front Oncol. 2020;10:575737 pubmed publisher
	Juno J, Tan H, Lee W, Reynaldi A, Kelly H, Wragg K, et al. Humoral and circulating follicular helper T cell responses in recovered patients with COVID-19. Nat Med. 2020;26:1428-1434 pubmed publisher
	Mastelic Gavillet B, Navarro Rodrigo B, Décombaz L, Wang H, Ercolano G, Ahmed R, et al. Adenosine mediates functional and metabolic suppression of peripheral and tumor-infiltrating CD8+ T cells. J Immunother Cancer. 2019;7:257 pubmed publisher
	Mederacke Y, Vondran F, Kollrich S, Schulde E, Schmitt R, Manns M, et al. Transient increase of activated regulatory T cells early after kidney transplantation. Sci Rep. 2019;9:1021 pubmed publisher
	Pissani F, Schulte B, Eller M, Schultz B, Ratto Kim S, Marovich M, et al. Modulation of Vaccine-Induced CD4 T Cell Functional Profiles by Changes in Components of HIV Vaccine Regimens in Humans. J Virol. 2018;92: pubmed publisher
	Moguche A, Musvosvi M, Penn Nicholson A, Plumlee C, Mearns H, Geldenhuys H, et al. Antigen Availability Shapes T Cell Differentiation and Function during Tuberculosis. Cell Host Microbe. 2017;21:695-706.e5 pubmed publisher
	Kerstein A, Schüler S, Cabral Marques O, Fazio J, Häsler R, Muller A, et al. Environmental factor and inflammation-driven alteration of the total peripheral T-cell compartment in granulomatosis with polyangiitis. J Autoimmun. 2017;78:79-91 pubmed publisher
	Cornelius C, Schöneweis K, Georgi F, Weber M, Niederberger V, Zieglmayer P, et al. Immunotherapy With the PreS-based Grass Pollen Allergy Vaccine BM32 Induces Antibody Responses Protecting Against Hepatitis B Infection. EBioMedicine. 2016;11:58-67 pubmed publisher
	Afshar M, Richards S, Mann D, Cross A, Smith G, Netzer G, et al. Acute immunomodulatory effects of binge alcohol ingestion. Alcohol. 2015;49:57-64 pubmed publisher
	Zenaro E, Donini M, Dusi S. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol. 2009;86:1393-401 pubmed publisher

product information

Antigen :

CD4

Apps. Abbrev. :

Flow Cytometry, ICC

Cat # :

300532

Clone :

RPA-T4

Item :

Brilliant Violet 421™ anti-human CD4

Isotype :

Mouse IgG1, κ

Other Names :

Size :

100 tests

Price (USD) :

345 USD

Reactivity :

Human, Chimpanzee

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV421

Application Notes :

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , and blocking of T cell activation 1,2 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

more info or order :

BioLegend product webpage