PerCP/Cyanine5.5 anti-human CD4 | BioLegend 300530 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-human CD4

catalog :

300530

quantity :

100 tests

price :

185 USD

clonality :

monoclonal

host :

mouse

conjugate :

PerCP/Cy5.5

clone name :

RPA-T4

reactivity :

human, mouse, chimpanzee

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 1d	Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
flow cytometry; mouse; loading ...; fig s4	Cheng L, Ma J, Li J, Li D, Li G, Li F, et al. Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs. J Clin Invest. 2017;127:269-279 pubmed publisher
flow cytometry; human	Llibre A, LÃ³pez MacÃas C, Marafioti T, Mehta H, Partridge A, Kanzig C, et al. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation. J Immunol. 2016;196:2085-94 pubmed publisher
flow cytometry; human	Sumitomo S, Fujio K, Okamura T, Morita K, Ishigaki K, Suzukawa K, et al. Transcription factor early growth response 3 is associated with the TGF-?1 expression and the regulatory activity of CD4-positive T cells in vivo. J Immunol. 2013;191:2351-9 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Washburn M, Wang Z, Walton A, Goedegebuure S, Figueroa D, Van Horn S, et al. T Cell- and Monocyte-Specific RNA-Sequencing Analysis in Septic and Nonseptic Critically Ill Patients and in Patients with Cancer. J Immunol. 2019;203:1897-1908 pubmed publisher
	DʼAntoni M, Paul R, Mitchell B, Kohorn L, Fischer L, Lefebvre E, et al. Improved Cognitive Performance and Reduced Monocyte Activation in Virally Suppressed Chronic HIV After Dual CCR2 and CCR5 Antagonism. J Acquir Immune Defic Syndr. 2018;79:108-116 pubmed publisher
	Pollack R, Jones R, Pertea M, Bruner K, Martin A, Thomas A, et al. Defective HIV-1 Proviruses Are Expressed and Can Be Recognized by Cytotoxic T Lymphocytes, which Shape the Proviral Landscape. Cell Host Microbe. 2017;21:494-506.e4 pubmed publisher
	Beyer M, Abdullah Z, Chemnitz J, Maisel D, Sander J, Lehmann C, et al. Tumor-necrosis factor impairs CD4(+) T cell-mediated immunological control in chronic viral infection. Nat Immunol. 2016;17:593-603 pubmed publisher
	Zakhour R, Tran D, Degaffe G, Bell C, Donnachie E, Zhang W, et al. Recent Thymus Emigrant CD4+ T Cells Predict HIV Disease Progression in Patients With Perinatally Acquired HIV. Clin Infect Dis. 2016;62:1029-1035 pubmed publisher
	Lu T, Yu S, Liu Y, Yin C, Ye J, Liu Z, et al. Aberrant Circulating Th17 Cells in Patients with B-Cell Non-Hodgkin's Lymphoma. PLoS ONE. 2016;11:e0148044 pubmed publisher
	Steichen A, Binstock B, Mishra B, Sharma J. C-type lectin receptor Clec4d plays a protective role in resolution of Gram-negative pneumonia. J Leukoc Biol. 2013;94:393-8 pubmed publisher
	Zenaro E, Donini M, Dusi S. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol. 2009;86:1393-401 pubmed publisher

product information

Antigen :

CD4

Apps. Abbrev. :

Flow Cytometry

Cat # :

300530

Clone :

RPA-T4

Item :

PerCP/Cyanine5.5 anti-human CD4

Isotype :

Mouse IgG1, κ

Other Names :

Size :

100 tests

Price (USD) :

185 USD

Reactivity :

Human, Chimpanzee

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Application Notes :

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , and blocking of T cell activation 1,2 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).

more info or order :

BioLegend product webpage