product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-human CD4
catalog :
300529
quantity :
25 tests
price :
105 USD
clonality :
monoclonal
host :
mouse
conjugate :
PerCP/Cy5.5
clone name :
RPA-T4
reactivity :
human, mouse, chimpanzee
application :
flow cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 1d
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Cheng L, Ma J, Li J, Li D, Li G, Li F, et al. Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs. J Clin Invest. 2017;127:269-279 pubmed publisher
  • flow cytometry; human
Llibre A, López Macías C, Marafioti T, Mehta H, Partridge A, Kanzig C, et al. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation. J Immunol. 2016;196:2085-94 pubmed publisher
  • flow cytometry; human
Sumitomo S, Fujio K, Okamura T, Morita K, Ishigaki K, Suzukawa K, et al. Transcription factor early growth response 3 is associated with the TGF-?1 expression and the regulatory activity of CD4-positive T cells in vivo. J Immunol. 2013;191:2351-9 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Woldemeskel B, Dykema A, Garliss C, Cherfils S, Smith K, Blankson J. CD4+ T cells from COVID-19 mRNA vaccine recipients recognize a conserved epitope present in diverse coronaviruses. J Clin Invest. 2022;132: pubmed publisher
Kim M, Cooper M, Jacobs M, Ritchey J, Hollaway J, Fehniger T, et al. CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy. JCI Insight. 2021;6: pubmed publisher
Harb H, Benamar M, Lai P, Contini P, Griffith J, Crestani E, et al. Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections. Immunity. 2021;54:1186-1199.e7 pubmed publisher
Schupp J, Christians A, Zimmer N, Gleue L, Jonuleit H, Helm M, et al. In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model. Int J Mol Sci. 2021;22: pubmed publisher
Srivastava S, Furlan S, Jaeger Ruckstuhl C, Sarvothama M, Berger C, Smythe K, et al. Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade. Cancer Cell. 2021;39:193-208.e10 pubmed publisher
Woldemeskel B, Kwaa A, Garliss C, Laeyendecker O, Ray S, Blankson J. Healthy donor T cell responses to common cold coronaviruses and SARS-CoV-2. J Clin Invest. 2020;130:6631-6638 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Walsh R, Lannan M, Wen Y, Wang X, Moreland C, Willency J, et al. Post-hoc assessment of the immunogenicity of three antibodies reveals distinct immune stimulatory mechanisms. MAbs. 2020;12:1764829 pubmed publisher
Tran T, Guha R, Portugal S, Skinner J, Ongoiba A, Bhardwaj J, et al. A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation. Immunity. 2019;51:750-765.e10 pubmed publisher
Washburn M, Wang Z, Walton A, Goedegebuure S, Figueroa D, Van Horn S, et al. T Cell- and Monocyte-Specific RNA-Sequencing Analysis in Septic and Nonseptic Critically Ill Patients and in Patients with Cancer. J Immunol. 2019;203:1897-1908 pubmed publisher
DʼAntoni M, Paul R, Mitchell B, Kohorn L, Fischer L, Lefebvre E, et al. Improved Cognitive Performance and Reduced Monocyte Activation in Virally Suppressed Chronic HIV After Dual CCR2 and CCR5 Antagonism. J Acquir Immune Defic Syndr. 2018;79:108-116 pubmed publisher
Pollack R, Jones R, Pertea M, Bruner K, Martin A, Thomas A, et al. Defective HIV-1 Proviruses Are Expressed and Can Be Recognized by Cytotoxic T Lymphocytes, which Shape the Proviral Landscape. Cell Host Microbe. 2017;21:494-506.e4 pubmed publisher
Beyer M, Abdullah Z, Chemnitz J, Maisel D, Sander J, Lehmann C, et al. Tumor-necrosis factor impairs CD4(+) T cell-mediated immunological control in chronic viral infection. Nat Immunol. 2016;17:593-603 pubmed publisher
Zakhour R, Tran D, Degaffe G, Bell C, Donnachie E, Zhang W, et al. Recent Thymus Emigrant CD4+ T Cells Predict HIV Disease Progression in Patients With Perinatally Acquired HIV. Clin Infect Dis. 2016;62:1029-1035 pubmed publisher
Lu T, Yu S, Liu Y, Yin C, Ye J, Liu Z, et al. Aberrant Circulating Th17 Cells in Patients with B-Cell Non-Hodgkin's Lymphoma. PLoS ONE. 2016;11:e0148044 pubmed publisher
Steichen A, Binstock B, Mishra B, Sharma J. C-type lectin receptor Clec4d plays a protective role in resolution of Gram-negative pneumonia. J Leukoc Biol. 2013;94:393-8 pubmed publisher
Zenaro E, Donini M, Dusi S. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol. 2009;86:1393-401 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
300529
Clone :
RPA-T4
Item :
PerCP/Cyanine5.5 anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
25 tests
Price (USD) :
105 USD
Reactivity :
Human, Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , blocking of T cell activation 1,2 , and spatial biology (IBEX) 10,11 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA