product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP anti-human CD4
catalog :
300527
quantity :
25 tests
price :
143 USD
clonality :
monoclonal
host :
mouse
conjugate :
PerCP
clone name :
RPA-T4
reactivity :
human, chimpanzee
application :
flow cytometry
more info or order :
citations: 21
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Hirschberger S, Strauß G, Effinger D, Marstaller X, Ferstl A, Müller M, et al. Very-low-carbohydrate diet enhances human T-cell immunity through immunometabolic reprogramming. EMBO Mol Med. 2021;13:e14323 pubmed publisher
Rajashekar J, Richard J, Beloor J, Prévost J, Anand S, Beaudoin Bussieres G, et al. Modulating HIV-1 envelope glycoprotein conformation to decrease the HIV-1 reservoir. Cell Host Microbe. 2021;29:904-916.e6 pubmed publisher
Le J, Park J, Ha V, Luong A, Branciamore S, Rodin A, et al. Single-Cell RNA-Seq Mapping of Human Thymopoiesis Reveals Lineage Specification Trajectories and a Commitment Spectrum in T Cell Development. Immunity. 2020;52:1105-1118.e9 pubmed publisher
Vallejo Gracia A, Chen I, Perrone R, Besnard E, Boehm D, Battivelli E, et al. FOXO1 promotes HIV latency by suppressing ER stress in T cells. Nat Microbiol. 2020;: pubmed publisher
Diao B, Wang C, Tan Y, Chen X, Liu Y, Ning L, et al. Reduction and Functional Exhaustion of T Cells in Patients With Coronavirus Disease 2019 (COVID-19). Front Immunol. 2020;11:827 pubmed publisher
Tran T, Guha R, Portugal S, Skinner J, Ongoiba A, Bhardwaj J, et al. A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation. Immunity. 2019;51:750-765.e10 pubmed publisher
Verboven K, Wouters K, Gaens K, Hansen D, Bijnen M, Wetzels S, et al. Abdominal subcutaneous and visceral adipocyte size, lipolysis and inflammation relate to insulin resistance in male obese humans. Sci Rep. 2018;8:4677 pubmed publisher
Venugopal G, Mueller M, Hartmann S, Steinfelder S. Differential immunomodulation in human monocytes versus macrophages by filarial cystatin. PLoS ONE. 2017;12:e0188138 pubmed publisher
Boehm D, Jeng M, Camus G, Gramatica A, Schwarzer R, Johnson J, et al. SMYD2-Mediated Histone Methylation Contributes to HIV-1 Latency. Cell Host Microbe. 2017;21:569-579.e6 pubmed publisher
Woodberry T, Loughland J, Minigo G, Burel J, Amante F, Piera K, et al. Early Immune Regulatory Changes in a Primary Controlled Human Plasmodium vivax Infection: CD1c+ Myeloid Dendritic Cell Maturation Arrest, Induction of the Kynurenine Pathway, and Regulatory T Cell Activation. Infect Immun. 2017;85: pubmed publisher
Wouters K, Gaens K, Bijnen M, Verboven K, Jocken J, Wetzels S, et al. Circulating classical monocytes are associated with CD11c+ macrophages in human visceral adipose tissue. Sci Rep. 2017;7:42665 pubmed publisher
Riner D, Ndombi E, Carter J, Omondi A, Kittur N, Kavere E, et al. Schistosoma mansoni Infection Can Jeopardize the Duration of Protective Levels of Antibody Responses to Immunizations against Hepatitis B and Tetanus Toxoid. PLoS Negl Trop Dis. 2016;10:e0005180 pubmed publisher
Ye H, Wang Y, Yan J, Jenson A. Characterization of the anti-inflammation mechanism for the AO herbal extract. Exp Mol Pathol. 2016;101:341-345 pubmed publisher
Nakaya H, Clutterbuck E, Kazmin D, Wang L, Cortese M, Bosinger S, et al. Systems biology of immunity to MF59-adjuvanted versus nonadjuvanted trivalent seasonal influenza vaccines in early childhood. Proc Natl Acad Sci U S A. 2016;113:1853-8 pubmed publisher
Petit N, Lambert Niclot S, Marcelin A, Garcia S, Marodon G. HIV Replication Is Not Controlled by CD8+ T Cells during the Acute Phase of the Infection in Humanized Mice. PLoS ONE. 2015;10:e0138420 pubmed publisher
Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed publisher
Bikker A, Kruize A, Wenting M, Versnel M, Bijlsma J, Lafeber F, et al. Increased interleukin (IL)-7R? expression in salivary glands of patients with primary Sjogren's syndrome is restricted to T cells and correlates with IL-7 expression, lymphocyte numbers and activity. Ann Rheum Dis. 2012;71:1027-33 pubmed publisher
Conti L, De Palma R, Rolla S, Boselli D, Rodolico G, Kaur S, et al. Th17 cells in multiple sclerosis express higher levels of JAK2, which increases their surface expression of IFN-?R2. J Immunol. 2012;188:1011-8 pubmed publisher
Smolders J, Peelen E, Thewissen M, Cohen Tervaert J, Menheere P, Hupperts R, et al. Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis. PLoS ONE. 2010;5:e15235 pubmed publisher
Zenaro E, Donini M, Dusi S. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol. 2009;86:1393-401 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
300527
Clone :
RPA-T4
Item :
PerCP anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
25 tests
Price (USD) :
143 USD
Reactivity :
Human, Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PERCP
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , blocking of T cell activation 1,2 , and spatial biology (IBEX) 10,11 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA