Pacific Blue™ anti-human CD4 | BioLegend 300521 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Pacific Blue™ anti-human CD4

catalog :

300521

quantity :

100 μg

price :

225 USD

clonality :

monoclonal

host :

mouse

conjugate :

Pacific Blue

clone name :

RPA-T4

reactivity :

human, chimpanzee

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; tbl 1	van der Velden V, Flores Montero J, Perez Andres M, Martin Ayuso M, Crespo O, Blanco E, et al. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2017;: pubmed publisher
flow cytometry; human; loading ...; fig 1d	van der Burgh R, Meeldijk J, Jongeneel L, Frenkel J, Bovenschen N, van Gijn M, et al. Reduced serpinB9-mediated caspase-1 inhibition can contribute to autoinflammatory disease. Oncotarget. 2016;7:19265-71 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Veatch J, Jesernig B, Kargl J, Fitzgibbon M, Lee S, Baik C, et al. Endogenous CD4+ T Cells Recognize Neoantigens in Lung Cancer Patients, Including Recurrent Oncogenic KRAS and ERBB2 (Her2) Driver Mutations. Cancer Immunol Res. 2019;7:910-922 pubmed publisher
	Ickrath P, Scherzad A, Kleinsasser N, Ginzkey C, Hagen R, Hackenberg S. Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture system. Biomed Rep. 2019;10:119-126 pubmed publisher
	Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, et al. Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis. Int J Mol Med. 2018;42:1116-1124 pubmed publisher
	Barman S, Kayama H, Okuzaki D, Ogino T, Osawa H, Matsuno H, et al. Identification of a human intestinal myeloid cell subset that regulates gut homeostasis. Int Immunol. 2016;28:533-545 pubmed
	KÃ¸llgaard T, Ugurel Becker S, Idorn M, Andersen M, Becker J, Straten P. Pre-Vaccination Frequencies of Th17 Cells Correlate with Vaccine-Induced T-Cell Responses to Survivin-Derived Peptide Epitopes. PLoS ONE. 2015;10:e0131934 pubmed publisher
	Qin H, Cho M, Haso W, Zhang L, Tasian S, Oo H, et al. Eradication of B-ALL using chimeric antigen receptor-expressing T cells targeting the TSLPR oncoprotein. Blood. 2015;126:629-39 pubmed publisher
	Frencher J, Shen H, Yan L, Wilson J, Freitag N, Rizzo A, et al. HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of VÎ³2VÎ´2 T cells. J Leukoc Biol. 2014;96:957-67 pubmed publisher
	Hebel K, Weinert S, Kuropka B, Knolle J, Kosak B, Jorch G, et al. CD4+ T cells from human neonates and infants are poised spontaneously to run a nonclassical IL-4 program. J Immunol. 2014;192:5160-70 pubmed publisher
	Boland J, Foulds G, Ahmedzai S, Pockley A. A preliminary evaluation of the effects of opioids on innate and adaptive human in vitro immune function. BMJ Support Palliat Care. 2014;4:357-67 pubmed publisher
	Jiang W, Younes S, Funderburg N, Mudd J, Espinosa E, Davenport M, et al. Cycling memory CD4+ T cells in HIV disease have a diverse T cell receptor repertoire and a phenotype consistent with bystander activation. J Virol. 2014;88:5369-80 pubmed publisher
	Zenaro E, Donini M, Dusi S. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol. 2009;86:1393-401 pubmed publisher

product information

Antigen :

CD4

Apps. Abbrev. :

Flow Cytometry

Cat # :

300521

Clone :

RPA-T4

Item :

Pacific Blue™ anti-human CD4

Isotype :

Mouse IgG1, κ

Other Names :

Size :

100 μg

Price (USD) :

225 USD

Reactivity :

Human, Chimpanzee

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Application Notes :

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , and blocking of T cell activation 1,2 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).

more info or order :

BioLegend product webpage