product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-human CD4
catalog :
300518
quantity :
100 tests
price :
265 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
RPA-T4
reactivity :
human, chimpanzee
application :
flow cytometry
more info or order :
citations: 25
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 3a
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
  • flow cytometry; human; loading ...; fig 5b
Kong X, Martinez Barricarte R, Kennedy J, Mele F, Lazarov T, Deenick E, et al. Disruption of an antimycobacterial circuit between dendritic and helper T cells in human SPPL2a deficiency. Nat Immunol. 2018;19:973-985 pubmed publisher
  • flow cytometry; human; loading ...; fig 6e
Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Seumois G, Zapardiel Gonzalo J, White B, Singh D, Schulten V, Dillon M, et al. Transcriptional Profiling of Th2 Cells Identifies Pathogenic Features Associated with Asthma. J Immunol. 2016;197:655-64 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Swadling L, Pallett L, Diniz M, Baker J, Amin O, Stegmann K, et al. Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence. Cell Rep. 2020;30:687-698.e6 pubmed publisher
Charpentier J, Chen D, Lapinski P, Turner J, Grigorova I, Swanson J, et al. Macropinocytosis drives T cell growth by sustaining the activation of mTORC1. Nat Commun. 2020;11:180 pubmed publisher
Leclerc M, Voilin E, Gros G, Corgnac S, de Montpreville V, Validire P, et al. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1. Nat Commun. 2019;10:3345 pubmed publisher
Tardif V, Muir R, Cubas R, Chakhtoura M, Wilkinson P, Metcalf T, et al. Adenosine deaminase-1 delineates human follicular helper T cell function and is altered with HIV. Nat Commun. 2019;10:823 pubmed publisher
Einkauf K, Lee G, Gao C, Sharaf R, Sun X, Hua S, et al. Intact HIV-1 proviruses accumulate at distinct chromosomal positions during prolonged antiretroviral therapy. J Clin Invest. 2019;129:988-998 pubmed publisher
Wefers C, Duiveman de Boer T, Yigit R, Zusterzeel P, van Altena A, Massuger L, et al. Survival of Ovarian Cancer Patients Is Independent of the Presence of DC and T Cell Subsets in Ascites. Front Immunol. 2018;9:3156 pubmed publisher
Yarzabek B, Zaitouna A, Olson E, Silva G, Geng J, Geretz A, et al. Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity. elife. 2018;7: pubmed publisher
Kuo H, Ahmad R, Lee G, Gao C, Chen H, Ouyang Z, et al. Anti-apoptotic Protein BIRC5 Maintains Survival of HIV-1-Infected CD4+ T Cells. Immunity. 2018;48:1183-1194.e5 pubmed publisher
Sayin I, Radtke A, Vella L, Jin W, Wherry E, Buggert M, et al. Spatial distribution and function of T follicular regulatory cells in human lymph nodes. J Exp Med. 2018;215:1531-1542 pubmed publisher
Kagoya Y, Nakatsugawa M, Saso K, Guo T, Anczurowski M, Wang C, et al. DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models. Nat Commun. 2018;9:1915 pubmed publisher
Geng J, Altman J, Krishnakumar S, Raghavan M. Empty conformers of HLA-B preferentially bind CD8 and regulate CD8+ T cell function. elife. 2018;7: pubmed publisher
Zhou Y, Du J, Hou H, Lu Y, Yu J, Mao L, et al. Application of ImmunoScore Model for the Differentiation between Active Tuberculosis and Latent Tuberculosis Infection as Well as Monitoring Anti-tuberculosis Therapy. Front Cell Infect Microbiol. 2017;7:457 pubmed publisher
Whitfield S, Taylor C, Risdall J, Griffiths G, Jones J, Williamson E, et al. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology. J Immunol. 2017;198:3989-3998 pubmed publisher
Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
Iannetta M, Zingaropoli M, Bellizzi A, Morreale M, Pontecorvo S, D Abramo A, et al. Natalizumab Affects T-Cell Phenotype in Multiple Sclerosis: Implications for JCV Reactivation. PLoS ONE. 2016;11:e0160277 pubmed publisher
Karlsson H, Svensson E, Gigg C, Jarvius M, Olsson Strömberg U, Savoldo B, et al. Evaluation of Intracellular Signaling Downstream Chimeric Antigen Receptors. PLoS ONE. 2015;10:e0144787 pubmed publisher
Canaday D, Sridaran S, Van Epps P, Aung H, Burant C, Nsereko M, et al. CD4+ T cell polyfunctional profile in HIV-TB coinfection are similar between individuals with latent and active TB infection. Tuberculosis (Edinb). 2015;95:470-5 pubmed publisher
Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, et al. Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential. J Am Heart Assoc. 2014;3:e000743 pubmed publisher
Qi Y, Operario D, Georas S, Mosmann T. The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin. PLoS ONE. 2012;7:e39072 pubmed publisher
Zenaro E, Donini M, Dusi S. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol. 2009;86:1393-401 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
300518
Clone :
RPA-T4
Item :
APC/Cyanine7 anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
100 tests
Price (USD) :
265 USD
Reactivity :
Human, Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , and blocking of T cell activation 1,2 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA