product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-human CD4
catalog :
300512
quantity :
100 tests
price :
292 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE/Cy7
clone name :
RPA-T4
reactivity :
human, chimpanzee
application :
flow cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2a
Wei C, Mei J, Tang L, Liu Y, Li D, Li M, et al. 1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis. Cell Death Dis. 2016;7:e2489 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Romero Olmedo A, Schulz A, Hochstätter S, Das Gupta D, Virta I, Hirseland H, et al. Induction of robust cellular and humoral immunity against SARS-CoV-2 after a third dose of BNT162b2 vaccine in previously unresponsive older adults. Nat Microbiol. 2022;7:195-199 pubmed publisher
Shiozawa S, Tsumiyama K, Miyazaki Y, Uto K, Sakurai K, Nakashima T, et al. DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus. iScience. 2022;25:103537 pubmed publisher
Albanese M, Ruhle A, Mittermaier J, Mejías Pérez E, Gapp M, Linder A, et al. Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analyses. Nat Methods. 2022;19:81-89 pubmed publisher
Min H, Moon J, Lee S, Lee A, Lee C, Lee J, et al. Expanded IL-22+ Group 3 Innate Lymphoid Cells and Role of Oxidized LDL-C in the Pathogenesis of Axial Spondyloarthritis with Dyslipidaemia. Immune Netw. 2021;21:e43 pubmed publisher
Muliaditan T, Halim L, Whilding L, Draper B, Achkova D, Kausar F, et al. Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors. Cell Rep Med. 2021;2:100457 pubmed publisher
Wu V, Nobles C, Kuri Cervantes L, McCormick K, Everett J, Nguyen S, et al. Assessment of HIV-1 integration in tissues and subsets across infection stages. JCI Insight. 2020;5: pubmed publisher
Leite N, Sintov E, Meissner T, Brehm M, Greiner D, Harlan D, et al. Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells. Cell Rep. 2020;32:107894 pubmed publisher
Frazzette N, Khodadadi Jamayran A, Doudican N, Santana A, Felsen D, Pavlick A, et al. Decreased cytotoxic T cells and TCR clonality in organ transplant recipients with squamous cell carcinoma. NPJ Precis Oncol. 2020;4:13 pubmed publisher
Preglej T, Hamminger P, Luu M, Bulat T, Andersen L, Göschl L, et al. Histone deacetylases 1 and 2 restrain CD4+ cytotoxic T lymphocyte differentiation. JCI Insight. 2020;5: pubmed publisher
Rousset F, Salmon P, Bredl S, Cherpin O, Coelho M, Myburgh R, et al. Optimizing Synthetic miRNA Minigene Architecture for Efficient miRNA Hairpin Concatenation and Multi-target Gene Knockdown. Mol Ther Nucleic Acids. 2018;14:351-363 pubmed publisher
Hassounah N, Malladi V, Huang Y, Freeman S, Beauchamp E, Koyama S, et al. Identification and characterization of an alternative cancer-derived PD-L1 splice variant. Cancer Immunol Immunother. 2019;68:407-420 pubmed publisher
Rochat M, Schlaepfer E, Kuster S, Li D, Audigé A, Ivic S, et al. Monitoring HIV DNA and cellular activation markers in HIV-infected humanized mice under cART. Virol J. 2018;15:191 pubmed publisher
Toepfner N, Herold C, Otto O, Rosendahl P, Jacobi A, Kr ter M, et al. Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood. elife. 2018;7: pubmed publisher
Yu N, Lambert S, Bornstein J, Nair R, Enerback C, Elder J. The Act1 D10N missense variant impairs CD40 signaling in human B-cells. Genes Immun. 2019;20:23-31 pubmed publisher
Terzioğlu G, Ormeci B, Turksoy O, Sayman C, Cinar N, Öztürk G, et al. Mitochondrial depletion in CD4+ and CD19+ peripheral lymphocytes in early stage Alzheimer's disease. Mech Ageing Dev. 2017;167:24-29 pubmed publisher
Audigé A, Rochat M, Li D, Ivic S, Fahrny A, Muller C, et al. Long-term leukocyte reconstitution in NSG mice transplanted with human cord blood hematopoietic stem and progenitor cells. BMC Immunol. 2017;18:28 pubmed publisher
Chung B, Kim K, Kim B, Doh K, Cho M, Yang C. Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction. PLoS ONE. 2015;10:e0145258 pubmed publisher
Chung B, Kim K, Kim B, Piao S, Lim S, Choi B, et al. Dysregulation of Th17 cells during the early post-transplant period in patients under calcineurin inhibitor based immunosuppression. PLoS ONE. 2012;7:e42011 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
300512
Clone :
RPA-T4
Item :
PE/Cyanine7 anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
100 tests
Price (USD) :
292 USD
Reactivity :
Human, Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE_CYANINE7
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , blocking of T cell activation 1,2 , and spatial biology (IBEX) 10,11 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA