PE/Cyanine5 anti-human CD4 | BioLegend 300509 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine5 anti-human CD4

catalog :

300509

quantity :

25 tests

price :

105 USD

clonality :

monoclonal

host :

mouse

conjugate :

PE-Cy5

clone name :

RPA-T4

reactivity :

human, chimpanzee

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	O Regan N, Steinfelder S, Venugopal G, Rao G, Lucius R, Srikantam A, et al. Brugia malayi microfilariae induce a regulatory monocyte/macrophage phenotype that suppresses innate and adaptive immune responses. PLoS Negl Trop Dis. 2014;8:e3206 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Li G, Zhang Z, Reszka Blanco N, Li F, Chi L, Ma J, et al. Specific Activation In Vivo of HIV-1 by a Bromodomain Inhibitor from Monocytic Cells in Humanized Mice under Antiretroviral Therapy. J Virol. 2019;93: pubmed publisher
	Rodriguez Meira A, Buck G, Clark S, Povinelli B, Alcolea V, Louka E, et al. Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing. Mol Cell. 2019;73:1292-1305.e8 pubmed publisher
	Jung J, Buisman S, Weersing E, Dethmers Ausema A, Zwart E, Schepers H, et al. CBX7 Induces Self-Renewal of Human Normal and Malignant Hematopoietic Stem and Progenitor Cells by Canonical and Non-canonical Interactions. Cell Rep. 2019;26:1906-1918.e8 pubmed publisher
	Bill M, B van Kooten Niekerk P, S Woll P, Laine Herborg L, Stidsholt Roug A, Hokland P, et al. Mapping the CLEC12A expression on myeloid progenitors in normal bone marrow; implications for understanding CLEC12A-related cancer stem cell biology. J Cell Mol Med. 2018;22:2311-2318 pubmed publisher
	Ryba M, Marek N, Hak Ł, Rybarczyk Kapturska K, Myśliwiec M, Trzonkowski P, et al. Anti-TNF rescue CD4+Foxp3+ regulatory T cells in patients with type 1 diabetes from effects mediated by TNF. Cytokine. 2011;55:353-61 pubmed publisher

product information

Antigen :

CD4

Apps. Abbrev. :

Flow Cytometry

Cat # :

300509

Clone :

RPA-T4

Item :

PE/Cyanine5 anti-human CD4

Isotype :

Mouse IgG1, κ

Other Names :

Size :

25 tests

Price (USD) :

105 USD

Reactivity :

Human, Chimpanzee

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_CYANINE5

Application Notes :

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , and blocking of T cell activation 1,2 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).

more info or order :

BioLegend product webpage