product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-human CD4
catalog :
300508
quantity :
100 tests
price :
60 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
RPA-T4
reactivity :
human, mouse, chimpanzee
application :
flow cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s10d
Lodygin D, Hermann M, Schweingruber N, Flügel Koch C, Watanabe T, Schlosser C, et al. β-Synuclein-reactive T cells induce autoimmune CNS grey matter degeneration. Nature. 2019;566:503-508 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Yang T, St John L, Garber H, Kerros C, Ruisaard K, Clise Dwyer K, et al. Membrane-Associated Proteinase 3 on Granulocytes and Acute Myeloid Leukemia Inhibits T Cell Proliferation. J Immunol. 2018;201:1389-1399 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Nadjsombati M, McGinty J, Lyons Cohen M, Jaffe J, DiPeso L, Schneider C, et al. Detection of Succinate by Intestinal Tuft Cells Triggers a Type 2 Innate Immune Circuit. Immunity. 2018;49:33-41.e7 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Deng Y, Cheng J, Fu B, Liu W, Chen G, Zhang Q, et al. Hepatic carcinoma-associated fibroblasts enhance immune suppression by facilitating the generation of myeloid-derived suppressor cells. Oncogene. 2017;36:1090-1101 pubmed publisher
  • flow cytometry; human; fig 2
Rapiteanu R, Davis L, Williamson J, Timms R, Paul Luzio J, Lehner P. A Genetic Screen Identifies a Critical Role for the WDR81-WDR91 Complex in the Trafficking and Degradation of Tetherin. Traffic. 2016;17:940-58 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Zeng Y, Liu C, Gong Y, Bai Z, Hou S, He J, et al. Single-Cell RNA Sequencing Resolves Spatiotemporal Development of Pre-thymic Lymphoid Progenitors and Thymus Organogenesis in Human Embryos. Immunity. 2019;51:930-948.e6 pubmed publisher
Bagadia P, Huang X, Liu T, Durai V, Grajales Reyes G, Nitschké M, et al. An Nfil3-Zeb2-Id2 pathway imposes Irf8 enhancer switching during cDC1 development. Nat Immunol. 2019;20:1174-1185 pubmed publisher
Xia Y, Gao Y, Wang B, Zhang H, Zhang Q. Optimizing the Method of Cell Separation from Bile of Patients with Cholangiocarcinoma for Flow Cytometry. Gastroenterol Res Pract. 2019;2019:5436961 pubmed publisher
Anderson N, Buffone A, Hammer D. T lymphocytes migrate upstream after completing the leukocyte adhesion cascade. Cell Adh Migr. 2019;13:163-168 pubmed publisher
Li B, SONG T, Wang F, Yin C, Li Z, Lin J, et al. Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion. Oncogenesis. 2019;8:17 pubmed publisher
Gärtner K, Luckner M, Wanner G, Zeidler R. Engineering extracellular vesicles as novel treatment options: exploiting herpesviral immunity in CLL. J Extracell Vesicles. 2019;8:1573051 pubmed publisher
Franchini D, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, et al. Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells. Cell Rep. 2019;26:94-107.e7 pubmed publisher
Miura M, Miyazato P, Satou Y, Tanaka Y, Bangham C. Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent. Wellcome Open Res. 2018;3:105 pubmed publisher
Barry K, Hsu J, Broz M, Cueto F, Binnewies M, Combes A, et al. A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments. Nat Med. 2018;24:1178-1191 pubmed publisher
Kuo H, Ahmad R, Lee G, Gao C, Chen H, Ouyang Z, et al. Anti-apoptotic Protein BIRC5 Maintains Survival of HIV-1-Infected CD4+ T Cells. Immunity. 2018;48:1183-1194.e5 pubmed publisher
Wang F, Li B, Wei Y, Zhao Y, Wang L, Zhang P, et al. Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression. Oncogenesis. 2018;7:41 pubmed publisher
Tankou S, Regev K, Healy B, Tjon E, Laghi L, Cox L, et al. A probiotic modulates the microbiome and immunity in multiple sclerosis. Ann Neurol. 2018;83:1147-1161 pubmed publisher
Chen J, Chernatynskaya A, Li J, Kimbrell M, Cassidy R, Perry D, et al. T cells display mitochondria hyperpolarization in human type 1 diabetes. Sci Rep. 2017;7:10835 pubmed publisher
Fu W, Qiu C, Zhou M, Zhu L, Yang Y, Qiu C, et al. Immune Activation Influences SAMHD1 Expression and Vpx-mediated SAMHD1 Degradation during Chronic HIV-1 Infection. Sci Rep. 2016;6:38162 pubmed publisher
Beyer M, Abdullah Z, Chemnitz J, Maisel D, Sander J, Lehmann C, et al. Tumor-necrosis factor impairs CD4(+) T cell-mediated immunological control in chronic viral infection. Nat Immunol. 2016;17:593-603 pubmed publisher
Scott J, Cunningham M, Naga O, Wirth J, EuDaly J, Gilkeson G. Estrogen Receptor α Deficiency Modulates TLR Ligand-Mediated PDC-TREM Expression in Plasmacytoid Dendritic Cells in Lupus-Prone Mice. J Immunol. 2015;195:5561-71 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
300508
Clone :
RPA-T4
Item :
PE anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
100 tests
Price (USD) :
60 USD
Reactivity :
Human, Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , and blocking of T cell activation 1,2 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA