product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human CD4
catalog :
300505
quantity :
25 tests
price :
17 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
RPA-T4
reactivity :
human, chimpanzee
application :
flow cytometry
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 3a
Trabanelli S, Lecciso M, Salvestrini V, Cavo M, Očadlíková D, Lemoli R, et al. PGE2-induced IDO1 inhibits the capacity of fully mature DCs to elicit an in vitro antileukemic immune response. J Immunol Res. 2015;2015:253191 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Beatson R, Parente Pereira A, Halim L, Cozzetto D, Hull C, Whilding L, et al. TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer. Cell Rep Med. 2021;2:100473 pubmed publisher
Kim M, Hardy M, Edgington Mitchell L, Ramarathinam S, Chung S, Russell A, et al. Hydroxychloroquine inhibits the mitochondrial antioxidant system in activated T cells. iScience. 2021;24:103509 pubmed publisher
Kim M, Cooper M, Jacobs M, Ritchey J, Hollaway J, Fehniger T, et al. CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy. JCI Insight. 2021;6: pubmed publisher
Hamilton J, Tsuchida C, Nguyen D, Shy B, McGarrigle E, Sandoval Espinoza C, et al. Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering. Cell Rep. 2021;35:109207 pubmed publisher
Zong D, Huang B, LI Y, Lu Y, Xiang N, Guo C, et al. Chromatin accessibility landscapes of immune cells in rheumatoid arthritis nominate monocytes in disease pathogenesis. BMC Biol. 2021;19:79 pubmed publisher
Vanoni G, Ercolano G, Candiani S, Rutigliani M, Lanata M, Derre L, et al. Human primed ILCPs support endothelial activation through NF-κB signaling. elife. 2021;10: pubmed publisher
Gong B, Zheng L, Lu Z, Huang J, Pu J, Pan S, et al. Mesenchymal stem cells negatively regulate CD4<sup>+</sup> T cell activation in patients with primary Sj&ouml;gren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway. Mol Med Rep. 2021;23: pubmed publisher
Pagel J, Twisselmann N, Rausch T, Waschina S, Hartz A, Steinbeis M, et al. Increased Regulatory T Cells Precede the Development of Bronchopulmonary Dysplasia in Preterm Infants. Front Immunol. 2020;11:565257 pubmed publisher
Handono K, Putra Gofur N, Nurdiana N, Kalim H, Wahono C, Poeranto S, et al. Role of Lymphocytes CD4/CD8 Ratio and Immunoglobulin G Cytomegalovirus as Potential Markers for Systemic Lupus Erythematosus Patients with Periodontal Disease. Eur J Dent. 2020;14:544-550 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Soldi R, Ghosh Halder T, Weston A, Thode T, Drenner K, Lewis R, et al. The novel reversible LSD1 inhibitor SP-2577 promotes anti-tumor immunity in SWItch/Sucrose-NonFermentable (SWI/SNF) complex mutated ovarian cancer. PLoS ONE. 2020;15:e0235705 pubmed publisher
Mothe B, Rosás Umbert M, Coll P, Manzardo C, Puertas M, Morón López S, et al. HIVconsv Vaccines and Romidepsin in Early-Treated HIV-1-Infected Individuals: Safety, Immunogenicity and Effect on the Viral Reservoir (Study BCN02). Front Immunol. 2020;11:823 pubmed publisher
Kang L, Oh E, Cho C, Kwon H, Lee C, Jeon J, et al. 3'-Sialyllactose prebiotics prevents skin inflammation via regulatory T cell differentiation in atopic dermatitis mouse models. Sci Rep. 2020;10:5603 pubmed publisher
Yang L, Xue X, Zeng T, Chen X, Zhao Q, Tang X, et al. Novel biallelic TRNT1 mutations lead to atypical SIFD and multiple immune defects. Genes Dis. 2020;7:128-137 pubmed publisher
Zhao Y, Lu F, Ye J, Ji M, Pang Y, Wang Y, et al. Myeloid-Derived Suppressor Cells and γδT17 Cells Contribute to the Development of Gastric MALT Lymphoma in H. felis-Infected Mice. Front Immunol. 2019;10:3104 pubmed publisher
Ickrath P, Scherzad A, Kleinsasser N, Ginzkey C, Hagen R, Hackenberg S. Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture system. Biomed Rep. 2019;10:119-126 pubmed publisher
Iio K, Kabata D, Iio R, Imai Y, Hatanaka M, Omori H, et al. Parathyroid hormone and premature thymus ageing in patients with chronic kidney disease. Sci Rep. 2019;9:813 pubmed publisher
Goletz C, Lischke T, Harnack U, Schiele P, Danielczyk A, Rühmann J, et al. Glyco-Engineered Anti-Human Programmed Death-Ligand 1 Antibody Mediates Stronger CD8 T Cell Activation Than Its Normal Glycosylated and Non-Glycosylated Counterparts. Front Immunol. 2018;9:1614 pubmed publisher
Sibener L, Fernandes R, Kolawole E, Carbone C, Liu F, McAffee D, et al. Isolation of a Structural Mechanism for Uncoupling T Cell Receptor Signaling from Peptide-MHC Binding. Cell. 2018;174:672-687.e27 pubmed publisher
Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, et al. Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis. Int J Mol Med. 2018;42:1116-1124 pubmed publisher
Alhaj Hussen K, Vu Manh T, Guimiot F, Nelson E, Chabaane E, Delord M, et al. Molecular and Functional Characterization of Lymphoid Progenitor Subsets Reveals a Bipartite Architecture of Human Lymphopoiesis. Immunity. 2017;47:680-696.e8 pubmed publisher
Meng Y, Zhou W, Jin L, Liu L, Chang K, Mei J, et al. RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization. Cell Death Dis. 2017;8:e3105 pubmed publisher
Rolandelli A, Hernández Del Pino R, Pellegrini J, Tateosian N, Amiano N, de la Barrera S, et al. The IL-17A rs2275913 single nucleotide polymorphism is associated with protection to tuberculosis but related to higher disease severity in Argentina. Sci Rep. 2017;7:40666 pubmed publisher
Wahl S, Drong A, Lehne B, Loh M, Scott W, Kunze S, et al. Epigenome-wide association study of body mass index, and the adverse outcomes of adiposity. Nature. 2017;541:81-86 pubmed publisher
Hansen E, Ransom M, Hesselberth J, Hosmane N, Capoferri A, Bruner K, et al. Diverse fates of uracilated HIV-1 DNA during infection of myeloid lineage cells. elife. 2016;5: pubmed publisher
Luo X, Nie J, Wang S, Chen Z, Chen W, Li D, et al. Poly(ADP-ribosyl)ation of FOXP3 Protein Mediated by PARP-1 Protein Regulates the Function of Regulatory T Cells. J Biol Chem. 2015;290:28675-82 pubmed publisher
Han L, Yang J, Wang X, Wu Q, Yin S, Li Z, et al. The E3 deubiquitinase USP17 is a positive regulator of retinoic acid-related orphan nuclear receptor ?t (ROR?t) in Th17 cells. J Biol Chem. 2014;289:25546-55 pubmed publisher
Tamai Y, Hasegawa A, Takamori A, Sasada A, Tanosaki R, Choi I, et al. Potential contribution of a novel Tax epitope-specific CD4+ T cells to graft-versus-Tax effect in adult T cell leukemia patients after allogeneic hematopoietic stem cell transplantation. J Immunol. 2013;190:4382-92 pubmed publisher
Zenaro E, Donini M, Dusi S. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol. 2009;86:1393-401 pubmed publisher
Choudhry V, Zhang M, Harris I, Sidorov I, Vu B, Dimitrov A, et al. Increased efficacy of HIV-1 neutralization by antibodies at low CCR5 surface concentration. Biochem Biophys Res Commun. 2006;348:1107-15 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry, SB
Cat # :
300505
Clone :
RPA-T4
Item :
FITC anti-human CD4
Isotype :
Mouse IgG1, κ
Other Names :
T4
Size :
25 tests
Price (USD) :
17 USD
Reactivity :
Human, Chimpanzee
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Application Notes :
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections 3,4,5 , blocking of T cell activation 1,2 , and spatial biology (IBEX) 10,11 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA