product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD3
catalog :
300473
quantity :
50 mg
price :
2354 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
UCHT1
reactivity :
human
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 6a
Benkhoucha M, Tran N, Breville G, Senoner I, Bradfield P, Papayannopoulou T, et al. CD4+c-Met+Itgα4+ T cell subset promotes murine neuroinflammation. J Neuroinflammation. 2022;19:103 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 1a
Shen X, Geng R, Li Q, Chen Y, Li S, Wang Q, et al. ACE2-independent infection of T lymphocytes by SARS-CoV-2. Signal Transduct Target Ther. 2022;7:83 pubmed publisher
  • flow cytometry; human
Ming S, Yin H, Li X, Gong S, Zhang G, Wu Y. GITR Promotes the Polarization of TFH-Like Cells in Helicobacter pylori-Positive Gastritis. Front Immunol. 2021;12:736269 pubmed publisher
  • flow cytometry; human; loading ...
Yue X, Petersen F, Shu Y, Kasper B, Magatsin J, Ahmadi M, et al. Transfer of PBMC From SSc Patients Induces Autoantibodies and Systemic Inflammation in Rag2-/-/IL2rg-/- Mice. Front Immunol. 2021;12:677970 pubmed publisher
  • flow cytometry; human; fig 5
Hawerkamp H, Domdey A, Radau L, Sewerin P, Oláh P, Homey B, et al. Tofacitinib downregulates antiviral immune defence in keratinocytes and reduces T cell activation. Arthritis Res Ther. 2021;23:144 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020;11:532684 pubmed publisher
  • flow cytometry; human; loading ...
Hood S, Cosma G, Foulds G, Johnson C, Reeder S, McArdle S, et al. Identifying prostate cancer and its clinical risk in asymptomatic men using machine learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Kim J, Lee J, Go H, Lim J, Oh J, Kim Y, et al. Clinical and histological significance of urinary CD11c+ macrophages in lupus nephritis. Arthritis Res Ther. 2020;22:173 pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Fischer M, Ruhnau J, Schulze J, Obst D, Floel A, Vogelgesang A. Spermine and spermidine modulate T-cell function in older adults with and without cognitive decline ex vivo. Aging (Albany NY). 2020;12:13716-13739 pubmed publisher
  • flow cytometry; human; loading ...; fig s12a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • mass cytometry; human; loading ...
Banga R, Rebecchini C, Procopio F, Noto A, Munoz O, Ioannidou K, et al. Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement. PLoS Pathog. 2019;15:e1007918 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Boscheinen J, Thomann S, Knipe D, Deluca N, Schuler Thurner B, Gross S, et al. Generation of an Oncolytic Herpes Simplex Virus 1 Expressing Human MelanA. Front Immunol. 2019;10:2 pubmed publisher
  • flow cytometry; human; fig 2a
  • immunocytochemistry; human; fig 2d
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Cai Y, Abdel Mohsen M, Tomescu C, Xue F, Wu G, Howell B, et al. BCL6 Inhibitor-Mediated Downregulation of Phosphorylated SAMHD1 and T Cell Activation Are Associated with Decreased HIV Infection and Reactivation. J Virol. 2019;93: pubmed publisher
. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell. 2022;185:916-938.e58 pubmed publisher
Texler B, Zollner A, Reinstadler V, Reider S, Macheiner S, Jelušić B, et al. Tofacitinib-Induced Modulation of Intestinal Adaptive and Innate Immunity and Factors Driving Cellular and Systemic Pharmacokinetics. Cell Mol Gastroenterol Hepatol. 2022;13:383-404 pubmed publisher
Martínez Fábregas J, Wang L, Pohler E, Cozzani A, Wilmes S, Kazemian M, et al. CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci. Cell Rep. 2020;33:108545 pubmed publisher
Ryu S, Lee E, Kim D, Kim Y, Chung D, Kim J, et al. Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis. Arthritis Res Ther. 2020;22:63 pubmed publisher
Etxeberria I, Bolaños E, Quetglas J, Gros A, Villanueva A, Palomero J, et al. Intratumor Adoptive Transfer of IL-12 mRNA Transiently Engineered Antitumor CD8+ T Cells. Cancer Cell. 2019;36:613-629.e7 pubmed publisher
Jiao S, Subudhi S, Aparicio A, Ge Z, Guan B, Miura Y, et al. Differences in Tumor Microenvironment Dictate T Helper Lineage Polarization and Response to Immune Checkpoint Therapy. Cell. 2019;179:1177-1190.e13 pubmed publisher
Shehata L, Maurer D, Wec A, Lilov A, Champney E, Sun T, et al. Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability. Cell Rep. 2019;28:3300-3308.e4 pubmed publisher
Leng T, Akther H, Hackstein C, Powell K, King T, Friedrich M, et al. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions. Cell Rep. 2019;28:3077-3091.e5 pubmed publisher
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
Pascutti M, Geerman S, Collins N, Brasser G, Nota B, Stark R, et al. Peripheral and systemic antigens elicit an expandable pool of resident memory CD8+ T cells in the bone marrow. Eur J Immunol. 2019;49:853-872 pubmed publisher
Pohlmeyer C, Gonzalez V, Irrinki A, Ramirez R, Li L, Mulato A, et al. Identification of NK Cell Subpopulations That Differentiate HIV-Infected Subject Cohorts with Diverse Levels of Virus Control. J Virol. 2019;93: pubmed publisher
Furie R, Werth V, Merola J, Stevenson L, Reynolds T, Naik H, et al. Monoclonal antibody targeting BDCA2 ameliorates skin lesions in systemic lupus erythematosus. J Clin Invest. 2019;129:1359-1371 pubmed publisher
Valle Casuso J, Angin M, Volant S, Passaes C, Monceaux V, Mikhailova A, et al. Cellular Metabolism Is a Major Determinant of HIV-1 Reservoir Seeding in CD4+ T Cells and Offers an Opportunity to Tackle Infection. Cell Metab. 2019;29:611-626.e5 pubmed publisher
Salou M, Legoux F, Gilet J, Darbois A, du Halgouet A, Alonso R, et al. A common transcriptomic program acquired in the thymus defines tissue residency of MAIT and NKT subsets. J Exp Med. 2019;216:133-151 pubmed publisher
van Montfoort N, Borst L, Korrer M, Sluijter M, Marijt K, Santegoets S, et al. NKG2A Blockade Potentiates CD8 T Cell Immunity Induced by Cancer Vaccines. Cell. 2018;175:1744-1755.e15 pubmed publisher
Ye W, Chew M, Hou J, Lai F, Leopold S, Loo H, et al. Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway. PLoS Pathog. 2018;14:e1007298 pubmed publisher
Dam E, Maier A, Hocking A, Carlin J, Buckner J. Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis. Front Immunol. 2018;9:1978 pubmed publisher
Tedesco D, Thapa M, Chin C, Ge Y, Gong M, Li J, et al. Alterations in Intestinal Microbiota Lead to Production of Interleukin 17 by Intrahepatic γδ T-Cell Receptor-Positive Cells and Pathogenesis of Cholestatic Liver Disease. Gastroenterology. 2018;154:2178-2193 pubmed publisher
Cribbs A, Hookway E, Wells G, Lindow M, Obad S, Oerum H, et al. Inhibition of histone H3K27 demethylases selectively modulates inflammatory phenotypes of natural killer cells. J Biol Chem. 2018;293:2422-2437 pubmed publisher
Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
Teigler J, Zelinskyy G, Eller M, Bolton D, Marovich M, Gordon A, et al. Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection. J Virol. 2017;91: pubmed publisher
Wu Z, Qin R, Wang L, Bosso M, Scherer M, Stamminger T, et al. Human Cytomegalovirus Particles Treated with Specific Antibodies Induce Intrinsic and Adaptive but Not Innate Immune Responses. J Virol. 2017;91: pubmed publisher
Djaoud Z, Guethlein L, Horowitz A, Azzi T, Nemat Gorgani N, Olive D, et al. Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells. J Exp Med. 2017;214:1827-1841 pubmed publisher
Lopes F, Bálint Å, Valvo S, Felce J, Hessel E, Dustin M, et al. Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages. J Cell Biol. 2017;216:1123-1141 pubmed publisher
product information
Antigen :
CD3
Apps. Abbrev. :
FC, CyTOF®, IHC-F, IP, Activ, WB
Cat # :
300473
Clone :
UCHT1
Item :
Ultra-LEAF™ Purified anti-human CD3
Isotype :
Mouse IgG1, κ
Other Names :
T3, CD3ε
Size :
50 mg
Price (USD) :
2354 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections^11, immunoprecipitation^1, activation of T cells^2,3,5, Western blotting^9, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA