product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human CD3
catalog :
300458
quantity :
100 μg
price :
78 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
UCHT1
reactivity :
human
application :
flow cytometry
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 5
Hawerkamp H, Domdey A, Radau L, Sewerin P, Oláh P, Homey B, et al. Tofacitinib downregulates antiviral immune defence in keratinocytes and reduces T cell activation. Arthritis Res Ther. 2021;23:144 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human; loading ...; fig 6a
Otsuka Y, Watanabe E, Shinya E, Okura S, Saeki H, Geijtenbeek T, et al. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells. J Immunol. 2018;201:3006-3016 pubmed publisher
  • flow cytometry; human; loading ...
Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
Jacoby E, Ben Yakir Blumkin M, Blumenfeld Kan S, Brody Y, Meir A, Melamed Book N, et al. Mitochondrial augmentation of CD34+ cells from healthy donors and patients with mitochondrial DNA disorders confers functional benefit. NPJ Regen Med. 2021;6:58 pubmed publisher
Molina M, Hoffman E, Stokes J, Kummet N, Smith K, Baker F, et al. Regulatory Dendritic Cells Induced by Bendamustine Are Associated With Enhanced Flt3 Expression and Alloreactive T-Cell Death. Front Immunol. 2021;12:699128 pubmed publisher
Wang C, Li Y, Jia L, Kim J, Li J, Deng P, et al. CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance. Cell Stem Cell. 2021;28:1597-1613.e7 pubmed publisher
Lu P, Wang S, Franzen C, Venkataraman G, McClure R, Li L, et al. Ibrutinib and venetoclax target distinct subpopulations of CLL cells: implication for residual disease eradication. Blood Cancer J. 2021;11:39 pubmed publisher
Huang G, Nampe D, Yi J, Gabrelow G, Negri K, Kamb A, et al. A multivariate, quantitative assay that disentangles key kinetic parameters of primary human T cell function in vitro. PLoS ONE. 2020;15:e0241421 pubmed publisher
Barresi V, Napoli S, Spampinato G, Condorelli D, Signorelli S. Dectin-1 and TIM3 Expression in Deep Vein Thrombosis of Lower Limbs (DVTLL). J Clin Med. 2020;9: pubmed publisher
Zhang B, Wang Y, Huang S, Sun J, Wang M, Ma W, et al. Photoswitchable CAR-T Cell Function In Vitro and In Vivo via a Cleavable Mediator. Cell Chem Biol. 2020;: pubmed publisher
Lavaert M, Valcke B, Vandekerckhove B, Leclercq G, Liang K, Taghon T. Conventional and Computational Flow Cytometry Analyses Reveal Sustained Human Intrathymic T Cell Development From Birth Until Puberty. Front Immunol. 2020;11:1659 pubmed publisher
Giron L, Colomb F, Papasavvas E, Azzoni L, Yin X, Fair M, et al. Interferon-α alters host glycosylation machinery during treated HIV infection. EBioMedicine. 2020;59:102945 pubmed publisher
Pierini S, Tanyi J, Simpkins F, George E, Uribe Herranz M, Drapkin R, et al. Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target. JCI Insight. 2020;5: pubmed publisher
Crawford M, Sinha S, Renavikar P, Borcherding N, Karandikar N. CD4 T cell-intrinsic role for the T helper 17 signature cytokine IL-17: Effector resistance to immune suppression. Proc Natl Acad Sci U S A. 2020;117:19408-19414 pubmed publisher
Le J, Park J, Ha V, Luong A, Branciamore S, Rodin A, et al. Single-Cell RNA-Seq Mapping of Human Thymopoiesis Reveals Lineage Specification Trajectories and a Commitment Spectrum in T Cell Development. Immunity. 2020;52:1105-1118.e9 pubmed publisher
Martín Fernandez M, Bravo García Morato M, Gruber C, Murias Loza S, Malik M, Alsohime F, et al. Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions. Cell Rep. 2020;31:107633 pubmed publisher
Constantinescu Bercu A, Grassi L, Frontini M, Salles Crawley I, Woollard K, Crawley J. Activated αIIbβ3 on platelets mediates flow-dependent NETosis via SLC44A2. elife. 2020;9: pubmed publisher
Jing L, Ott M, Church C, Kulikauskas R, Ibrani D, Iyer J, et al. Prevalent and Diverse Intratumoral Oncoprotein-Specific CD8+ T Cells within Polyomavirus-Driven Merkel Cell Carcinomas. Cancer Immunol Res. 2020;8:648-659 pubmed publisher
Wu D, Lv J, Zhao R, Wu Z, Zheng D, Shi J, et al. PSCA is a target of chimeric antigen receptor T cells in gastric cancer. Biomark Res. 2020;8:3 pubmed publisher
Katsuyama E, Suárez Fueyo A, Bradley S, Mizui M, Marin A, Mulki L, et al. The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections. Cell Rep. 2020;30:112-123.e4 pubmed publisher
Urlaub D, Zhao S, Blank N, Bergner R, Claus M, Tretter T, et al. Activation of natural killer cells by rituximab in granulomatosis with polyangiitis. Arthritis Res Ther. 2019;21:277 pubmed publisher
Zhang C, Yue C, Herrmann A, Song J, Egelston C, Wang T, et al. STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth. Cell Metab. 2020;31:148-161.e5 pubmed publisher
Levring T, Kongsbak Wismann M, Rode A, Al Jaberi F, Lopez D, Met O, et al. Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells. Sci Rep. 2019;9:16725 pubmed publisher
Anderson N, Buffone A, Hammer D. T lymphocytes migrate upstream after completing the leukocyte adhesion cascade. Cell Adh Migr. 2019;13:163-168 pubmed publisher
Good Z, Borges L, Vivanco Gonzalez N, Sahaf B, Samusik N, Tibshirani R, et al. Proliferation tracing with single-cell mass cytometry optimizes generation of stem cell memory-like T cells. Nat Biotechnol. 2019;37:259-266 pubmed publisher
Mayassi T, Ladell K, Gudjonson H, McLaren J, Shaw D, Tran M, et al. Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease. Cell. 2019;176:967-981.e19 pubmed publisher
Ajith A, Portik Dobos V, Nguyen Lefebvre A, Callaway C, Horuzsko D, Kapoor R, et al. HLA-G dimer targets Granzyme B pathway to prolong human renal allograft survival. FASEB J. 2019;33:5220-5236 pubmed publisher
Saraiva D, Jacinto A, Borralho P, Braga S, Cabral M. HLA-DR in Cytotoxic T Lymphocytes Predicts Breast Cancer Patients' Response to Neoadjuvant Chemotherapy. Front Immunol. 2018;9:2605 pubmed publisher
Tognarelli S, Wirsching S, von Metzler I, Rais B, Jacobs B, Serve H, et al. Enhancing the Activation and Releasing the Brakes: A Double Hit Strategy to Improve NK Cell Cytotoxicity Against Multiple Myeloma. Front Immunol. 2018;9:2743 pubmed publisher
Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, et al. Immunological properties of neural crest cells derived from human induced pluripotent stem cells. Stem Cells Dev. 2018;: pubmed publisher
Sibener L, Fernandes R, Kolawole E, Carbone C, Liu F, McAffee D, et al. Isolation of a Structural Mechanism for Uncoupling T Cell Receptor Signaling from Peptide-MHC Binding. Cell. 2018;174:672-687.e27 pubmed publisher
Zhong J, Scholz T, Yau A, Guérard S, Huffmeier U, Burkhardt H, et al. Mannan-induced Nos2 in macrophages enhances IL-17-driven psoriatic arthritis by innate lymphocytes. Sci Adv. 2018;4:eaas9864 pubmed publisher
Lichtenegger F, Rothe M, Schnorfeil F, Deiser K, Krupka C, Augsberger C, et al. Targeting LAG-3 and PD-1 to Enhance T Cell Activation by Antigen-Presenting Cells. Front Immunol. 2018;9:385 pubmed publisher
Toepfner N, Herold C, Otto O, Rosendahl P, Jacobi A, Kr ter M, et al. Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood. elife. 2018;7: pubmed publisher
Meraviglia S, Lo Presti E, Tosolini M, La Mendola C, Orlando V, Todaro M, et al. Distinctive features of tumor-infiltrating ?? T lymphocytes in human colorectal cancer. Oncoimmunology. 2017;6:e1347742 pubmed publisher
Gerstner S, Kohler W, Heidkamp G, Purbojo A, Uchida S, Ekici A, et al. Specific phenotype and function of CD56-expressing innate immune cell subsets in human thymus. J Leukoc Biol. 2016;100:1297-1310 pubmed
Laurent A, Bindslev N, Johansson B, Berg L. Synergistic effects of ethanol and isopentenyl pyrophosphate on expansion of ?? T cells in synovial fluid from patients with arthritis. PLoS ONE. 2014;9:e103683 pubmed publisher
Headland S, Jones H, D Sa A, Perretti M, Norling L. Cutting-edge analysis of extracellular microparticles using ImageStream(X) imaging flow cytometry. Sci Rep. 2014;4:5237 pubmed publisher
Gutzeit C, Nagy N, Gentile M, Lyberg K, Gumz J, Vallhov H, et al. Exosomes derived from Burkitt's lymphoma cell lines induce proliferation, differentiation, and class-switch recombination in B cells. J Immunol. 2014;192:5852-62 pubmed publisher
Siegers G, Felizardo T, Mathieson A, Kosaka Y, Wang X, Medin J, et al. Anti-leukemia activity of in vitro-expanded human gamma delta T cells in a xenogeneic Ph+ leukemia model. PLoS ONE. 2011;6:e16700 pubmed publisher
Kim J, Lim H, Kang S, Hillsamer P, Kim C. Human CD57+ germinal center-T cells are the major helpers for GC-B cells and induce class switch recombination. BMC Immunol. 2005;6:3 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300458
Clone :
UCHT1
Item :
APC anti-human CD3
Isotype :
Mouse IgG1, κ
Other Names :
T3, CD3ε
Size :
100 μg
Price (USD) :
78 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections^11, immunoprecipitation^1, activation of T cells^2,3,5, Western blotting^9, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA