product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-human CD3
catalog :
300433
quantity :
25 tests
price :
190 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV421
clone name :
UCHT1
reactivity :
human
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...
Yue X, Petersen F, Shu Y, Kasper B, Magatsin J, Ahmadi M, et al. Transfer of PBMC From SSc Patients Induces Autoantibodies and Systemic Inflammation in Rag2-/-/IL2rg-/- Mice. Front Immunol. 2021;12:677970 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020;11:532684 pubmed publisher
Georg P, Astaburuaga García R, Bonaguro L, Brumhard S, Michalick L, Lippert L, et al. Complement activation induces excessive T cell cytotoxicity in severe COVID-19. Cell. 2022;185:493-512.e25 pubmed publisher
Chen Y, Yan B, Xu F, Hui Z, Zhao G, Liu J, et al. Neoadjuvant chemoimmunotherapy in resectable stage IIIA/IIIB non-small cell lung cancer. Transl Lung Cancer Res. 2021;10:2193-2204 pubmed publisher
Caduff N, McHugh D, Rieble L, Forconi C, Ong echa J, Oluoch P, et al. KSHV infection drives poorly cytotoxic CD56-negative natural killer cell differentiation in vivo upon KSHV/EBV dual infection. Cell Rep. 2021;35:109056 pubmed publisher
Kunishita Y, Yoshimi R, Kamiyama R, Kishimoto D, Yoshida K, Hashimoto E, et al. TRIM21 Dysfunction Enhances Aberrant B-Cell Differentiation in Autoimmune Pathogenesis. Front Immunol. 2020;11:98 pubmed publisher
Han L, Zhang Q, Dail M, Shi C, Cavazos A, Ruvolo V, et al. Concomitant targeting of BCL2 with venetoclax and MAPK signaling with cobimetinib in acute myeloid leukemia models. Haematologica. 2019;: pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
Franchini D, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, et al. Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells. Cell Rep. 2019;26:94-107.e7 pubmed publisher
Yoshitomi H, Kobayashi S, Miyagawa Hayashino A, Okahata A, Doi K, Nishitani K, et al. Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat Commun. 2018;9:3762 pubmed publisher
Idorn M, Olsen M, Halldórsdóttir H, Skadborg S, Pedersen M, Høgdall C, et al. Improved migration of tumor ascites lymphocytes to ovarian cancer microenvironment by CXCR2 transduction. Oncoimmunology. 2018;7:e1412029 pubmed publisher
Horn L, Ciavattone N, Atkinson R, Woldergerima N, Wolf J, Clements V, et al. CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized mice. Oncotarget. 2017;8:57964-57980 pubmed publisher
Baguma R, Penn Nicholson A, Smit E, Erasmus M, Day J, Makhethe L, et al. Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population. PLoS ONE. 2017;12:e0184563 pubmed publisher
Svensson A, Patzi Churqui M, Schlüter K, Lind L, Eriksson K. Maturation-dependent expression of AIM2 in human B-cells. PLoS ONE. 2017;12:e0183268 pubmed publisher
Ott P, Hu Z, Keskin D, Shukla S, Sun J, Bozym D, et al. An immunogenic personal neoantigen vaccine for patients with melanoma. Nature. 2017;547:217-221 pubmed publisher
Audigé A, Rochat M, Li D, Ivic S, Fahrny A, Muller C, et al. Long-term leukocyte reconstitution in NSG mice transplanted with human cord blood hematopoietic stem and progenitor cells. BMC Immunol. 2017;18:28 pubmed publisher
Tyler C, McCarthy N, Lindsay J, Stagg A, Moser B, Eberl M. Antigen-Presenting Human γδ T Cells Promote Intestinal CD4+ T Cell Expression of IL-22 and Mucosal Release of Calprotectin. J Immunol. 2017;198:3417-3425 pubmed publisher
Lundell A, Nordström I, Andersson K, Lundqvist C, Telemo E, Nava S, et al. IFN type I and II induce BAFF secretion from human decidual stromal cells. Sci Rep. 2017;7:39904 pubmed publisher
Li J, Jie H, Lei Y, Gildener Leapman N, Trivedi S, Green T, et al. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-518 pubmed publisher
Laurent A, Bindslev N, Johansson B, Berg L. Synergistic effects of ethanol and isopentenyl pyrophosphate on expansion of ?? T cells in synovial fluid from patients with arthritis. PLoS ONE. 2014;9:e103683 pubmed publisher
Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013;190:27-35 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC, ICC, IHC-F
Cat # :
300433
Clone :
UCHT1
Item :
Brilliant Violet 421™ anti-human CD3
Isotype :
Mouse IgG1, κ
Other Names :
T3, CD3ε
Size :
25 tests
Price (USD) :
190 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV421
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections^11, immunoprecipitation^1, activation of T cells^2,3,5, Western blotting^9, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA