LEAF™ Purified anti-human CD3 | BioLegend 300432 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD3

catalog :

300432

quantity :

1 mg

price :

300 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

UCHT1

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 7

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1a	Cooper G, Ostridge K, Khakoo S, Wilkinson T, Staples K. Human CD49a+ Lung Natural Killer Cell Cytotoxicity in Response to Influenza A Virus. Front Immunol. 2018;9:1671 pubmed publisher
blocking or activating experiments; human; loading ...; fig 2c	Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
flow cytometry; human; loading ...; fig s1	Djaoud Z, Guethlein L, Horowitz A, Azzi T, Nemat Gorgani N, Olive D, et al. Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells. J Exp Med. 2017;214:1827-1841 pubmed publisher
flow cytometry; human; fig s2a	Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
flow cytometry; human; loading ...; fig 1	Seumois G, Zapardiel Gonzalo J, White B, Singh D, Schulten V, Dillon M, et al. Transcriptional Profiling of Th2 Cells Identifies Pathogenic Features Associated with Asthma. J Immunol. 2016;197:655-64 pubmed publisher
flow cytometry; human; loading ...	Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
	Laurent A, Bindslev N, Johansson B, Berg L. Synergistic effects of ethanol and isopentenyl pyrophosphate on expansion of ?? T cells in synovial fluid from patients with arthritis. PLoS ONE. 2014;9:e103683 pubmed publisher

product information

Antigen :

CD3

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Activ, IHC, IP, Western Blotting

Cat # :

300432

Clone :

UCHT1

Item :

LEAF™ Purified anti-human CD3

Isotype :

Mouse IgG1, κ

Other Names :

T3, CD3ε

Size :

1 mg

Price (USD) :

300 USD

Reactivity :

Human, Cross-Reactivity: Chimpanzee

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections 4,6,7 and formalin-fixed paraffin-embedded sections 11 , immunoprecipitation 1 , activation of T cells 2,3,5 , and Western blotting 9 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).