product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-human CD3
catalog :
300430
quantity :
100 tests
price :
382 USD
clonality :
monoclonal
host :
mouse
conjugate :
PerCP/Cy5.5
clone name :
UCHT1
reactivity :
human
application :
flow cytometry, blocking or activating experiments
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 6c, 6f
Petley E, Koay H, Henderson M, Sek K, Todd K, Keam S, et al. MAIT cells regulate NK cell-mediated tumor immunity. Nat Commun. 2021;12:4746 pubmed publisher
  • flow cytometry; human; loading ...; fig s12a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • blocking or activating experiments; human; 2:100; fig 7
Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, et al. TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun. 2015;6:6329 pubmed publisher
  • flow cytometry; human
Armour K, Smith C, Ip N, Ellison C, Kirton C, Wilkes A, et al. Clearance of human IgG1-sensitised red blood cells in vivo in humans relates to the in vitro properties of antibodies from alternative cell lines. PLoS ONE. 2014;9:e109463 pubmed publisher
Rajakumar S, Grandal I, Minden M, Hitzler J, Guidos C, Danska J. Targeted blockade of immune mechanisms inhibit B precursor acute lymphoblastic leukemia cell invasion of the central nervous system. Cell Rep Med. 2021;2:100470 pubmed publisher
Yu H, Yang W, Huang J, Miao X, Wang B, Ren X, et al. GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure. Cell Death Dis. 2021;13:1 pubmed publisher
Chen M, Li Y, Wu Y, Xie S, Ma J, Yue J, et al. Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer. Cancers (Basel). 2021;13: pubmed publisher
Portillo A, Hogg R, Poznanski S, Rojas E, Cashell N, Hammill J, et al. Expanded human NK cells armed with CAR uncouple potent anti-tumor activity from off-tumor toxicity against solid tumors. iScience. 2021;24:102619 pubmed publisher
Xu S, Chaudhary O, Rodríguez Morales P, Sun X, Chen D, Zappasodi R, et al. Uptake of oxidized lipids by the scavenger receptor CD36 promotes lipid peroxidation and dysfunction in CD8+ T cells in tumors. Immunity. 2021;54:1561-1577.e7 pubmed publisher
Fels J, Maurer D, Herbert A, Wirchnianski A, Vergnolle O, Cross R, et al. Protective neutralizing antibodies from human survivors of Crimean-Congo hemorrhagic fever. Cell. 2021;184:3486-3501.e21 pubmed publisher
Khuzwayo S, Mthembu M, Meermeier E, Prakadan S, Kazer S, Bassett T, et al. MR1-Restricted MAIT Cells From The Human Lung Mucosal Surface Have Distinct Phenotypic, Functional, and Transcriptomic Features That Are Preserved in HIV Infection. Front Immunol. 2021;12:631410 pubmed publisher
Poznanski S, Singh K, Ritchie T, Aguiar J, Fan I, Portillo A, et al. Metabolic flexibility determines human NK cell functional fate in the tumor microenvironment. Cell Metab. 2021;33:1205-1220.e5 pubmed publisher
Leylek R, Alcántara Hernández M, Granja J, Chavez M, Perez K, Diaz O, et al. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity. Cell Rep. 2020;32:108180 pubmed publisher
Landgraf K, Williams S, Steiger D, Gebhart D, Lok S, Martin D, et al. convertibleCARs: A chimeric antigen receptor system for flexible control of activity and antigen targeting. Commun Biol. 2020;3:296 pubmed publisher
Turner A, Dronamraju R, Potjewyd F, James K, Winecoff D, Kirchherr J, et al. Evaluation of EED Inhibitors as a Class of PRC2-Targeted Small Molecules for HIV Latency Reversal. ACS Infect Dis. 2020;6:1719-1733 pubmed publisher
Vendrame E, Seiler C, Ranganath T, Zhao N, Vergara R, Alary M, et al. TIGIT is upregulated by HIV-1 infection and marks a highly functional adaptive and mature subset of natural killer cells. AIDS. 2020;34:801-813 pubmed publisher
Etxeberria I, Bolaños E, Quetglas J, Gros A, Villanueva A, Palomero J, et al. Intratumor Adoptive Transfer of IL-12 mRNA Transiently Engineered Antitumor CD8+ T Cells. Cancer Cell. 2019;36:613-629.e7 pubmed publisher
Shehata L, Maurer D, Wec A, Lilov A, Champney E, Sun T, et al. Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability. Cell Rep. 2019;28:3300-3308.e4 pubmed publisher
Shehata L, Wieland Alter W, Maurer D, Chen E, Connor R, Wright P, et al. Systematic comparison of respiratory syncytial virus-induced memory B cell responses in two anatomical compartments. Nat Commun. 2019;10:1126 pubmed publisher
Gorman J, Hundhausen C, Kinsman M, Arkatkar T, Allenspach E, Clough C, et al. The TYK2-P1104A Autoimmune Protective Variant Limits Coordinate Signals Required to Generate Specialized T Cell Subsets. Front Immunol. 2019;10:44 pubmed publisher
Herrera F, Valerio M, Berthold D, Tawadros T, Meuwly J, Vallet V, et al. 50-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial. Int J Radiat Oncol Biol Phys. 2019;103:320-334 pubmed publisher
Shen M, Chen Y, Xu L, Zhu R, Xue X, Tsai Y, et al. Increased infiltration of macrophages to radioresistant lung cancer cells contributes to the development of the additional resistance of tumor cells to the cytotoxic effects of NK cells. Int J Oncol. 2018;53:317-328 pubmed publisher
Alcántara Hernández M, Leylek R, Wagar L, Engleman E, Keler T, Marinkovich M, et al. High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization. Immunity. 2017;47:1037-1050.e6 pubmed publisher
Sumitomo S, Nagafuchi Y, Tsuchida Y, Tsuchiya H, Ota M, Ishigaki K, et al. A gene module associated with dysregulated TCR signaling pathways in CD4+ T cell subsets in rheumatoid arthritis. J Autoimmun. 2018;89:21-29 pubmed publisher
Xu L, Chen X, Shen M, Yang D, Fang L, Weng G, et al. Inhibition of IL-6-JAK/Stat3 signaling in castration-resistant prostate cancer cells enhances the NK cell-mediated cytotoxicity via alteration of PD-L1/NKG2D ligand levels. Mol Oncol. 2018;12:269-286 pubmed publisher
Korner C, Simoneau C, Schommers P, Granoff M, Ziegler M, Hölzemer A, et al. HIV-1-Mediated Downmodulation of HLA-C Impacts Target Cell Recognition and Antiviral Activity of NK Cells. Cell Host Microbe. 2017;22:111-119.e4 pubmed publisher
Dulberger C, McMurtrey C, Hölzemer A, Neu K, Liu V, Steinbach A, et al. Human Leukocyte Antigen F Presents Peptides and Regulates Immunity through Interactions with NK Cell Receptors. Immunity. 2017;46:1018-1029.e7 pubmed publisher
Shoda H, Nagafuchi Y, Tsuchida Y, Sakurai K, Sumitomo S, Fujio K, et al. Increased serum concentrations of IL-1 beta, IL-21 and Th17 cells in overweight patients with rheumatoid arthritis. Arthritis Res Ther. 2017;19:111 pubmed publisher
Nakachi S, Sumitomo S, Tsuchida Y, Tsuchiya H, Kono M, Kato R, et al. Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis. Arthritis Res Ther. 2017;19:97 pubmed publisher
Ciarlo E, Heinonen T, Herderschee J, Fenwick C, Mombelli M, Le Roy D, et al. Impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo. Sci Rep. 2016;6:37944 pubmed publisher
Ye C, Wang W, Cheng L, Li G, Wen M, Wang Q, et al. Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice. J Virol. 2017;91: pubmed publisher
Yeung Y, Foletti D, Deng X, Abdiche Y, Strop P, Glanville J, et al. Germline-encoded neutralization of a Staphylococcus aureus virulence factor by the human antibody repertoire. Nat Commun. 2016;7:13376 pubmed publisher
Pritchett J, Green J, Thomm A, Knox K, Verneris M, Lund T. CD4+ T Cells Coexpressing CD134 (OX40) Harbor Significantly Increased Levels of Human Herpesvirus 6B DNA Following Umbilical Cord Blood Transplantation. J Infect Dis. 2016;214:1911-1915 pubmed
Nagafuchi Y, Shoda H, Sumitomo S, Nakachi S, Kato R, Tsuchida Y, et al. Immunophenotyping of rheumatoid arthritis reveals a linkage between HLA-DRB1 genotype, CXCR4 expression on memory CD4(+) T cells, and disease activity. Sci Rep. 2016;6:29338 pubmed publisher
Gross C, Schulte Mecklenbeck A, Rünzi A, Kuhlmann T, Posevitz Fejfár A, Schwab N, et al. Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation. Proc Natl Acad Sci U S A. 2016;113:E2973-82 pubmed publisher
Teirlinck A, Roestenberg M, Bijker E, Hoffman S, Sauerwein R, Scholzen A. Plasmodium falciparum Infection of Human Volunteers Activates Monocytes and CD16+ Dendritic Cells and Induces Upregulation of CD16 and CD1c Expression. Infect Immun. 2015;83:3732-9 pubmed publisher
Li J, Jie H, Lei Y, Gildener Leapman N, Trivedi S, Green T, et al. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-518 pubmed publisher
Laurent A, Bindslev N, Johansson B, Berg L. Synergistic effects of ethanol and isopentenyl pyrophosphate on expansion of ?? T cells in synovial fluid from patients with arthritis. PLoS ONE. 2014;9:e103683 pubmed publisher
Poonia B, Pauza C. Levels of CD56+TIM-3- effector CD8 T cells distinguish HIV natural virus suppressors from patients receiving antiretroviral therapy. PLoS ONE. 2014;9:e88884 pubmed publisher
Ribot J, Ribeiro S, Correia D, Sousa A, Silva Santos B. Human ?? thymocytes are functionally immature and differentiate into cytotoxic type 1 effector T cells upon IL-2/IL-15 signaling. J Immunol. 2014;192:2237-43 pubmed publisher
Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013;190:27-35 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300430
Clone :
UCHT1
Item :
PerCP/Cyanine5.5 anti-human CD3
Isotype :
Mouse IgG1, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
382 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections^11, immunoprecipitation^1, activation of T cells^2,3,5, Western blotting^9, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA