product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-human CD3
catalog :
300426
quantity :
100 tests
price :
292 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
UCHT1
reactivity :
human
application :
flow cytometry
more info or order :
citations: 27
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 6a
Benkhoucha M, Tran N, Breville G, Senoner I, Bradfield P, Papayannopoulou T, et al. CD4+c-Met+Itgα4+ T cell subset promotes murine neuroinflammation. J Neuroinflammation. 2022;19:103 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 1a
Shen X, Geng R, Li Q, Chen Y, Li S, Wang Q, et al. ACE2-independent infection of T lymphocytes by SARS-CoV-2. Signal Transduct Target Ther. 2022;7:83 pubmed publisher
  • flow cytometry; human; loading ...; fig 6h
Mayassi T, Ladell K, Gudjonson H, McLaren J, Shaw D, Tran M, et al. Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease. Cell. 2019;176:967-981.e19 pubmed publisher
Woldemeskel B, Dykema A, Garliss C, Cherfils S, Smith K, Blankson J. CD4+ T cells from COVID-19 mRNA vaccine recipients recognize a conserved epitope present in diverse coronaviruses. J Clin Invest. 2022;132: pubmed publisher
Pušnik J, Richter E, Schulte B, Dolscheid Pommerich R, Bode C, Putensen C, et al. Memory B cells targeting SARS-CoV-2 spike protein and their dependence on CD4+ T cell help. Cell Rep. 2021;35:109320 pubmed publisher
Maleki K, Tauriainen J, García M, Kerkman P, Christ W, Dias J, et al. MAIT cell activation is associated with disease severity markers in acute hantavirus infection. Cell Rep Med. 2021;2:100220 pubmed publisher
Junker F, Gulati P, Wessels U, Seeber S, Stubenrauch K, Codarri Deak L, et al. A human receptor occupancy assay to measure anti-PD-1 binding in patients with prior anti-PD-1. Cytometry A. 2021;99:832-843 pubmed publisher
Duhen R, Ballesteros Merino C, Frye A, Tran E, Rajamanickam V, Chang S, et al. Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells. Nat Commun. 2021;12:1047 pubmed publisher
Sokal A, Chappert P, Barba Spaeth G, Roeser A, Fourati S, Azzaoui I, et al. Maturation and persistence of the anti-SARS-CoV-2 memory B cell response. Cell. 2021;184:1201-1213.e14 pubmed publisher
Wang K, Chen W, Zhang Z, Deng Y, Lian J, Du P, et al. CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells. Signal Transduct Target Ther. 2020;5:283 pubmed publisher
Lee J, Sheen J, Lim O, Lee Y, Ryu J, Shin D, et al. Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy. Sci Rep. 2020;10:17753 pubmed publisher
Woldemeskel B, Kwaa A, Garliss C, Laeyendecker O, Ray S, Blankson J. Healthy donor T cell responses to common cold coronaviruses and SARS-CoV-2. J Clin Invest. 2020;130:6631-6638 pubmed publisher
Lim J. Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysis. Sci Rep. 2020;10:14897 pubmed publisher
Lavaert M, Valcke B, Vandekerckhove B, Leclercq G, Liang K, Taghon T. Conventional and Computational Flow Cytometry Analyses Reveal Sustained Human Intrathymic T Cell Development From Birth Until Puberty. Front Immunol. 2020;11:1659 pubmed publisher
Sim M, Lu J, Spencer M, Hopkins F, Tran E, Rosenberg S, et al. High-affinity oligoclonal TCRs define effective adoptive T cell therapy targeting mutant KRAS-G12D. Proc Natl Acad Sci U S A. 2020;117:12826-12835 pubmed publisher
Del Alcazar D, Wang Y, He C, Wendel B, Del Rio Estrada P, Lin J, et al. Mapping the Lineage Relationship between CXCR5+ and CXCR5- CD4+ T Cells in HIV-Infected Human Lymph Nodes. Cell Rep. 2019;28:3047-3060.e7 pubmed publisher
Manser A, Scherenschlich N, Thöns C, Hengel H, Timm J, Uhrberg M. KIR Polymorphism Modulates the Size of the Adaptive NK Cell Pool in Human Cytomegalovirus-Infected Individuals. J Immunol. 2019;203:2301-2309 pubmed publisher
Oll Hurtado M, Wolbert J, Fisher J, Flutter B, Stafford S, Barton J, et al. Tumor infiltrating lymphocytes expanded from pediatric neuroblastoma display heterogeneity of phenotype and function. PLoS ONE. 2019;14:e0216373 pubmed publisher
Xia Y, Gao Y, Wang B, Zhang H, Zhang Q. Optimizing the Method of Cell Separation from Bile of Patients with Cholangiocarcinoma for Flow Cytometry. Gastroenterol Res Pract. 2019;2019:5436961 pubmed publisher
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
Buchan S, Dou L, Remer M, Booth S, Dunn S, Lai C, et al. Antibodies to Costimulatory Receptor 4-1BB Enhance Anti-tumor Immunity via T Regulatory Cell Depletion and Promotion of CD8 T Cell Effector Function. Immunity. 2018;49:958-970.e7 pubmed publisher
Macal M, Tam M, Hesser C, Di Domizio J, Léger P, Gilliet M, et al. CD28 Deficiency Enhances Type I IFN Production by Murine Plasmacytoid Dendritic Cells. J Immunol. 2016;196:1900-9 pubmed publisher
Sharkey A, Xiong S, Kennedy P, Gardner L, Farrell L, Chazara O, et al. Tissue-Specific Education of Decidual NK Cells. J Immunol. 2015;195:3026-32 pubmed publisher
Li J, Jie H, Lei Y, Gildener Leapman N, Trivedi S, Green T, et al. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-518 pubmed publisher
Laurent A, Bindslev N, Johansson B, Berg L. Synergistic effects of ethanol and isopentenyl pyrophosphate on expansion of ?? T cells in synovial fluid from patients with arthritis. PLoS ONE. 2014;9:e103683 pubmed publisher
Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013;190:27-35 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300426
Clone :
UCHT1
Item :
APC/Cyanine7 anti-human CD3
Isotype :
Mouse IgG1, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
292 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections^11, immunoprecipitation^1, activation of T cells^2,3,5, Western blotting^9, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA