product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-human CD3
catalog :
300420
quantity :
100 tests
price :
278 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE/Cy7
clone name :
UCHT1
reactivity :
human
application :
flow cytometry
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...
Andreano E, Nicastri E, Paciello I, Pileri P, Manganaro N, Piccini G, et al. Extremely potent human monoclonal antibodies from COVID-19 convalescent patients. Cell. 2021;184:1821-1835.e16 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Di Blasi D, Boldanova T, Mori L, Terracciano L, Heim M, De Libero G. Unique T-Cell Populations Define Immune-Inflamed Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol. 2020;9:195-218 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Mayassi T, Ladell K, Gudjonson H, McLaren J, Shaw D, Tran M, et al. Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease. Cell. 2019;176:967-981.e19 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
  • flow cytometry; human; fig s2a
Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Seumois G, Zapardiel Gonzalo J, White B, Singh D, Schulten V, Dillon M, et al. Transcriptional Profiling of Th2 Cells Identifies Pathogenic Features Associated with Asthma. J Immunol. 2016;197:655-64 pubmed publisher
  • flow cytometry; human; 1:100
Leone D, Kozakowski N, Kornauth C, Waidacher T, Neudert B, Loeffler A, et al. The Phenotypic Characterization of the Human Renal Mononuclear Phagocytes Reveal a Co-Ordinated Response to Injury. PLoS ONE. 2016;11:e0151674 pubmed publisher
  • flow cytometry; human
Fernandez L, Valentin J, Zalacain M, Leung W, Patino Garcia A, Perez Martinez A. Activated and expanded natural killer cells target osteosarcoma tumor initiating cells in an NKG2D-NKG2DL dependent manner. Cancer Lett. 2015;368:54-63 pubmed publisher
Ramirez N, Lee J, Zheng Y, Li L, Dennis B, Chen D, et al. ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS-ERK-AP-1 T cell signaling-transcriptional axis. Nat Commun. 2022;13:1109 pubmed publisher
Vijayakumar B, Boustani K, Ogger P, Papadaki A, Tonkin J, Orton C, et al. Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease. Immunity. 2022;55:542-556.e5 pubmed publisher
Todorova D, Zhang Y, Chen Q, Liu J, He J, Fu X, et al. hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts. EBioMedicine. 2020;62:103120 pubmed publisher
Leylek R, Alcántara Hernández M, Granja J, Chavez M, Perez K, Diaz O, et al. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity. Cell Rep. 2020;32:108180 pubmed publisher
Zeng J, Wu Y, Ren C, Bonanno J, Shen A, Shea D, et al. Therapeutic base editing of human hematopoietic stem cells. Nat Med. 2020;26:535-541 pubmed publisher
Zhao Y, Lu F, Ye J, Ji M, Pang Y, Wang Y, et al. Myeloid-Derived Suppressor Cells and γδT17 Cells Contribute to the Development of Gastric MALT Lymphoma in H. felis-Infected Mice. Front Immunol. 2019;10:3104 pubmed publisher
Weisberg S, Carpenter D, Chait M, Dogra P, Gartrell Corrado R, Chen A, et al. Tissue-Resident Memory T Cells Mediate Immune Homeostasis in the Human Pancreas through the PD-1/PD-L1 Pathway. Cell Rep. 2019;29:3916-3932.e5 pubmed publisher
Vela M, del Rosal T, Perez Martinez A, Valentín J, Casas I, Pozo F, et al. Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation. Sci Rep. 2019;9:18792 pubmed publisher
Moore N, Chevillet J, Healey L, McBrine C, Doty D, Santos J, et al. A Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular Therapies. Sci Rep. 2019;9:15101 pubmed publisher
Roman Rodriguez F, Ugalde L, Alvarez L, Díez B, Ramirez M, Risueño C, et al. NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia. Cell Stem Cell. 2019;25:607-621.e7 pubmed publisher
Lamichhane R, Schneider M, de la Harpe S, Harrop T, Hannaway R, Dearden P, et al. TCR- or Cytokine-Activated CD8+ Mucosal-Associated Invariant T Cells Are Rapid Polyfunctional Effectors That Can Coordinate Immune Responses. Cell Rep. 2019;28:3061-3076.e5 pubmed publisher
Manser A, Scherenschlich N, Thöns C, Hengel H, Timm J, Uhrberg M. KIR Polymorphism Modulates the Size of the Adaptive NK Cell Pool in Human Cytomegalovirus-Infected Individuals. J Immunol. 2019;203:2301-2309 pubmed publisher
Goel G, Tye Din J, Qiao S, Russell A, Mayassi T, Ciszewski C, et al. Cytokine release and gastrointestinal symptoms after gluten challenge in celiac disease. Sci Adv. 2019;5:eaaw7756 pubmed publisher
Ickrath P, Scherzad A, Kleinsasser N, Ginzkey C, Hagen R, Hackenberg S. Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture system. Biomed Rep. 2019;10:119-126 pubmed publisher
Iio K, Kabata D, Iio R, Imai Y, Hatanaka M, Omori H, et al. Parathyroid hormone and premature thymus ageing in patients with chronic kidney disease. Sci Rep. 2019;9:813 pubmed publisher
Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, et al. Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis. Int J Mol Med. 2018;42:1116-1124 pubmed publisher
Rosskopf S, Leitner J, Paster W, Morton L, Hagedoorn R, Steinberger P, et al. A Jurkat 76 based triple parameter reporter system to evaluate TCR functions and adoptive T cell strategies. Oncotarget. 2018;9:17608-17619 pubmed publisher
Palazzo A, Herter S, Grosmaire L, Jones R, Frey C, Limani F, et al. The PI3Kδ-Selective Inhibitor Idelalisib Minimally Interferes with Immune Effector Function Mediated by Rituximab or Obinutuzumab and Significantly Augments B Cell Depletion In Vivo. J Immunol. 2018;200:2304-2312 pubmed publisher
Howson L, Napolitani G, Shepherd D, Ghadbane H, Kurupati P, Preciado Llanes L, et al. MAIT cell clonal expansion and TCR repertoire shaping in human volunteers challenged with Salmonella Paratyphi A. Nat Commun. 2018;9:253 pubmed publisher
Suan D, Kräutler N, Mååg J, Butt D, Bourne K, Hermes J, et al. CCR6 Defines Memory B Cell Precursors in Mouse and Human Germinal Centers, Revealing Light-Zone Location and Predominant Low Antigen Affinity. Immunity. 2017;47:1142-1153.e4 pubmed publisher
Gladkikh A, Potashnikova D, Tatarskiy V, Yastrebova M, Khamidullina A, Barteneva N, et al. Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70. Cancer Med. 2017;6:2984-2997 pubmed publisher
Meraviglia S, Lo Presti E, Tosolini M, La Mendola C, Orlando V, Todaro M, et al. Distinctive features of tumor-infiltrating ?? T lymphocytes in human colorectal cancer. Oncoimmunology. 2017;6:e1347742 pubmed publisher
Verma K, Jyotsana N, Buenting I, Luther S, Pfanne A, Thum T, et al. miR-625-3p is upregulated in CD8+ T cells during early immune reconstitution after allogeneic stem cell transplantation. PLoS ONE. 2017;12:e0183828 pubmed publisher
Leone D, Peschel A, Brown M, Schachner H, Ball M, Gyuraszova M, et al. Surface LAMP-2 Is an Endocytic Receptor That Diverts Antigen Internalized by Human Dendritic Cells into Highly Immunogenic Exosomes. J Immunol. 2017;199:531-546 pubmed publisher
Taraldsrud E, Fevang B, Jørgensen S, Moltu K, Hilden V, Taskén K, et al. Defective IL-4 signaling in T cells defines severe common variable immunodeficiency. J Autoimmun. 2017;81:110-119 pubmed publisher
Pollack R, Jones R, Pertea M, Bruner K, Martin A, Thomas A, et al. Defective HIV-1 Proviruses Are Expressed and Can Be Recognized by Cytotoxic T Lymphocytes, which Shape the Proviral Landscape. Cell Host Microbe. 2017;21:494-506.e4 pubmed publisher
Beyer A, Muench M. Comparison of Human Hematopoietic Reconstitution in Different Strains of Immunodeficient Mice. Stem Cells Dev. 2017;26:102-112 pubmed publisher
Cartellieri M, Feldmann A, Koristka S, Arndt C, Loff S, Ehninger A, et al. Switching CAR T cells on and off: a novel modular platform for retargeting of T cells to AML blasts. Blood Cancer J. 2016;6:e458 pubmed publisher
Comte D, Karampetsou M, Kis Toth K, Yoshida N, Bradley S, Mizui M, et al. Engagement of SLAMF3 enhances CD4+ T-cell sensitivity to IL-2 and favors regulatory T-cell polarization in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 2016;113:9321-6 pubmed publisher
Nagafuchi Y, Shoda H, Sumitomo S, Nakachi S, Kato R, Tsuchida Y, et al. Immunophenotyping of rheumatoid arthritis reveals a linkage between HLA-DRB1 genotype, CXCR4 expression on memory CD4(+) T cells, and disease activity. Sci Rep. 2016;6:29338 pubmed publisher
Usero L, Sanchez A, Pizarro E, Xufré C, Marti M, Jaraquemada D, et al. Interleukin-13 Pathway Alterations Impair Invariant Natural Killer T-Cell-Mediated Regulation of Effector T Cells in Type 1 Diabetes. Diabetes. 2016;65:2356-66 pubmed publisher
Petit N, Lambert Niclot S, Marcelin A, Garcia S, Marodon G. HIV Replication Is Not Controlled by CD8+ T Cells during the Acute Phase of the Infection in Humanized Mice. PLoS ONE. 2015;10:e0138420 pubmed publisher
Ishigaki K, Shoda H, Kochi Y, Yasui T, Kadono Y, Tanaka S, et al. Quantitative and qualitative characterization of expanded CD4+ T cell clones in rheumatoid arthritis patients. Sci Rep. 2015;5:12937 pubmed publisher
Mashiko S, Bouguermouh S, Rubio M, Baba N, Bissonnette R, Sarfati M. Human mast cells are major IL-22 producers in patients with psoriasis and atopic dermatitis. J Allergy Clin Immunol. 2015;136:351-9.e1 pubmed publisher
Li J, Jie H, Lei Y, Gildener Leapman N, Trivedi S, Green T, et al. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-518 pubmed publisher
Laurent A, Bindslev N, Johansson B, Berg L. Synergistic effects of ethanol and isopentenyl pyrophosphate on expansion of ?? T cells in synovial fluid from patients with arthritis. PLoS ONE. 2014;9:e103683 pubmed publisher
Cartellieri M, Koristka S, Arndt C, Feldmann A, Stamova S, von Bonin M, et al. A novel ex vivo isolation and expansion procedure for chimeric antigen receptor engrafted human T cells. PLoS ONE. 2014;9:e93745 pubmed publisher
Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013;190:27-35 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300420
Clone :
UCHT1
Item :
PE/Cyanine7 anti-human CD3
Isotype :
Mouse IgG1, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
278 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE_CYANINE7
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections^11, immunoprecipitation^1, activation of T cells^2,3,5, Western blotting^9, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA