LEAF™ Purified anti-human CD3 | BioLegend 300413 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD3

catalog :

300413

quantity :

50 μg

price :

40 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

UCHT1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, blocking or activating experiments

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1a	Cooper G, Ostridge K, Khakoo S, Wilkinson T, Staples K. Human CD49a+ Lung Natural Killer Cell Cytotoxicity in Response to Influenza A Virus. Front Immunol. 2018;9:1671 pubmed publisher
blocking or activating experiments; human; loading ...; fig 2c	Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
flow cytometry; human; loading ...; fig s1	Djaoud Z, Guethlein L, Horowitz A, Azzi T, Nemat Gorgani N, Olive D, et al. Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells. J Exp Med. 2017;214:1827-1841 pubmed publisher
flow cytometry; human; loading ...; fig 5a	Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
flow cytometry; human; loading ...; fig s1a	Lopes F, BÃ¡lint Å, Valvo S, Felce J, Hessel E, Dustin M, et al. Membrane nanoclusters of FcÎ³RI segregate from inhibitory SIRPÎ± upon activation of human macrophages. J Cell Biol. 2017;216:1123-1141 pubmed publisher
flow cytometry; human; loading ...	Pinto Cardoso S, Lozupone C, Briceno O, Alva Hernández S, Téllez N, Adriana A, et al. Fecal Bacterial Communities in treated HIV infected individuals on two antiretroviral regimens. Sci Rep. 2017;7:43741 pubmed publisher
flow cytometry; human; loading ...	Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
flow cytometry; human; 1:100; loading ...; fig 1	Briceno O, Pinto Cardoso S, Rodríguez Bernabe N, Murakami Ogasawara A, Reyes Teran G. Gut Homing CD4+ and CD8+ T-Cell Frequencies in HIV Infected Individuals on Antiretroviral Treatment. PLoS ONE. 2016;11:e0166496 pubmed publisher
flow cytometry; human; loading ...	Cerny D, Thi Le D, The T, Zuest R, Kg S, Velumani S, et al. Complete human CD1a deficiency on Langerhans cells due to a rare point mutation in the coding sequence. J Allergy Clin Immunol. 2016;138:1709-1712.e11 pubmed publisher
flow cytometry; human; fig s2a	Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
flow cytometry; human; loading ...; fig 1	Seumois G, Zapardiel Gonzalo J, White B, Singh D, Schulten V, Dillon M, et al. Transcriptional Profiling of Th2 Cells Identifies Pathogenic Features Associated with Asthma. J Immunol. 2016;197:655-64 pubmed publisher
flow cytometry; human; loading ...; fig 1d	Goldstein J, Burlion A, Zaragoza B, Sendeyo K, Polansky J, Huehn J, et al. Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression. PLoS ONE. 2016;11:e0153682 pubmed publisher
flow cytometry; human; loading ...	Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
flow cytometry; human; 1:100	Leone D, Kozakowski N, Kornauth C, Waidacher T, Neudert B, Loeffler A, et al. The Phenotypic Characterization of the Human Renal Mononuclear Phagocytes Reveal a Co-Ordinated Response to Injury. PLoS ONE. 2016;11:e0151674 pubmed publisher
flow cytometry; mouse; 1:100	Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
flow cytometry; human; fig 2	Schnorfeil F, Lichtenegger F, Emmerig K, Schlueter M, Neitz J, Draenert R, et al. T cells are functionally not impaired in AML: increased PD-1 expression is only seen at time of relapse and correlates with a shift towards the memory T cell compartment. J Hematol Oncol. 2015;8:93 pubmed publisher
flow cytometry; human	Boer M, Prins C, van Meijgaarden K, van Dissel J, Ottenhoff T, Joosten S. Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults. Clin Vaccine Immunol. 2015;22:778-88 pubmed publisher
flow cytometry; human	van der Waart A, Fredrix H, van der Voort R, Schaap N, Hobo W, Dolstra H. siRNA silencing of PD-1 ligands on dendritic cell vaccines boosts the expansion of minor histocompatibility antigen-specific CD8(+) T cells in NOD/SCID/IL2Rg(null) mice. Cancer Immunol Immunother. 2015;64:645-54 pubmed publisher
blocking or activating experiments; human; 2:100; fig 7	Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, et al. TGF-Î²3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun. 2015;6:6329 pubmed publisher
flow cytometry; human	Laurent A, Bindslev N, Johansson B, Berg L. Synergistic effects of ethanol and isopentenyl pyrophosphate on expansion of ?? T cells in synovial fluid from patients with arthritis. PLoS ONE. 2014;9:e103683 pubmed publisher
chromatin immunoprecipitation; human western blot; human; fig 4a	Paster W, Brockmeyer C, Fu G, Simister P, de Wet B, Martinez RiaÃ±o A, et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol. 2013;190:3749-56 pubmed publisher
	Li J, Jie H, Lei Y, Gildener Leapman N, Trivedi S, Green T, et al. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-518 pubmed publisher
	Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013;190:27-35 pubmed publisher
	Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed

product information

Antigen :

CD3

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Activ, IHC, IP, Western Blotting

Cat # :

300413

Clone :

UCHT1

Item :

LEAF™ Purified anti-human CD3

Isotype :

Mouse IgG1, κ

Other Names :

T3, CD3ε

Size :

50 μg

Price (USD) :

40 USD

Reactivity :

Human, Cross-Reactivity: Chimpanzee

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections 4,6,7 and formalin-fixed paraffin-embedded sections 11 , immunoprecipitation 1 , activation of T cells 2,3,5 , and Western blotting 9 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).