product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human CD3
catalog :
300405
quantity :
25 tests
price :
22 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
UCHT1
reactivity :
human
application :
flow cytometry
more info or order :
citations: 69
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:250; loading ...; fig 6c
Götzke H, Kilisch M, Martínez Carranza M, Sograte Idrissi S, Rajavel A, Schlichthaerle T, et al. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019;10:4403 pubmed publisher
  • flow cytometry; human
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Duhen R, Ballesteros Merino C, Frye A, Tran E, Rajamanickam V, Chang S, et al. Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells. Nat Commun. 2021;12:1047 pubmed publisher
Vanoni G, Ercolano G, Candiani S, Rutigliani M, Lanata M, Derre L, et al. Human primed ILCPs support endothelial activation through NF-κB signaling. elife. 2021;10: pubmed publisher
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Shin C, Byun J, Lee K, Kim B, Noh Y, Tran N, et al. Exosomal miRNA-19a and miRNA-614 Induced by Air Pollutants Promote Proinflammatory M1 Macrophage Polarization via Regulation of RORα Expression in Human Respiratory Mucosal Microenvironment. J Immunol. 2020;205:3179-3190 pubmed publisher
Dyson M, Masters E, Pazeraitis D, Perera R, Syrjänen J, Surade S, et al. Beyond affinity: selection of antibody variants with optimal biophysical properties and reduced immunogenicity from mammalian display libraries. MAbs. 2020;12:1829335 pubmed publisher
Bhattacharya S, Calar K, Evans C, Petrasko M, de la Puente P. Bioengineering the Oxygen-Deprived Tumor Microenvironment Within a Three-Dimensional Platform for Studying Tumor-Immune Interactions. Front Bioeng Biotechnol. 2020;8:1040 pubmed publisher
Ping Y, Li F, Nan S, Zhang D, Shi X, Shan J, et al. Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv. Front Cell Dev Biol. 2020;8:803 pubmed publisher
Huang N, Fan X, Zaleta Rivera K, Nguyen T, Zhou J, Luo Y, et al. Natural display of nuclear-encoded RNA on the cell surface and its impact on cell interaction. Genome Biol. 2020;21:225 pubmed publisher
Jin S, Li R, Chen M, Yu C, Tang L, Liu Y, et al. Single-cell transcriptomic analysis defines the interplay between tumor cells, viral infection, and the microenvironment in nasopharyngeal carcinoma. Cell Res. 2020;30:950-965 pubmed publisher
Chen Y, Yin J, Li W, Li H, Chen D, Zhang C, et al. Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma. Cell Res. 2020;: pubmed publisher
Bennstein S, Weinhold S, Manser A, Scherenschlich N, Noll A, Raba K, et al. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells. elife. 2020;9: pubmed publisher
Kwok I, Becht E, Xia Y, Ng M, Teh Y, Tan L, et al. Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor. Immunity. 2020;: pubmed publisher
Jiang G, Shi L, Zheng X, Zhang X, Wu K, Liu B, et al. Androgen receptor affects the response to immune checkpoint therapy by suppressing PD-L1 in hepatocellular carcinoma. Aging (Albany NY). 2020;12:11466-11484 pubmed publisher
Jiang Y, Wei X, Guan J, Qin S, Wang Z, Lu H, et al. COVID-19 pneumonia: CD8+ T and NK cells are decreased in number but compensatory increased in cytotoxic potential. Clin Immunol. 2020;218:108516 pubmed publisher
Klemm F, Maas R, Bowman R, Kornete M, Soukup K, Nassiri S, et al. Interrogation of the Microenvironmental Landscape in Brain Tumors Reveals Disease-Specific Alterations of Immune Cells. Cell. 2020;181:1643-1660.e17 pubmed publisher
Chang Z, Hou A, Chen Y. Engineering primary T cells with chimeric antigen receptors for rewired responses to soluble ligands. Nat Protoc. 2020;15:1507-1524 pubmed publisher
Rendeiro A, Krausgruber T, Fortelny N, Zhao F, Penz T, Farlik M, et al. Chromatin mapping and single-cell immune profiling define the temporal dynamics of ibrutinib response in CLL. Nat Commun. 2020;11:577 pubmed publisher
Suzuki D, Flahou C, Yoshikawa N, Stirblyte I, Hayashi Y, Sawaguchi A, et al. iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity. Stem Cell Reports. 2020;14:49-59 pubmed publisher
Wang J, Liu J, Tian F, Zhan Y, Kong D. Cyclin-dependent kinase 9 expression and its association with CD8+ T cell infiltration in microsatellite-stable colorectal cancer. Oncol Lett. 2019;18:6046-6056 pubmed publisher
Del Alcazar D, Wang Y, He C, Wendel B, Del Rio Estrada P, Lin J, et al. Mapping the Lineage Relationship between CXCR5+ and CXCR5- CD4+ T Cells in HIV-Infected Human Lymph Nodes. Cell Rep. 2019;28:3047-3060.e7 pubmed publisher
Coufal S, Galanova N, Bajer L, Gajdarova Z, Schierova D, Jiraskova Zakostelska Z, et al. Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response. Cells. 2019;8: pubmed publisher
Ryzhov S, May T, Dziodzio J, Emery I, Lucas F, Leclerc A, et al. Number of Circulating CD 73-Expressing Lymphocytes Correlates With Survival After Cardiac Arrest. J Am Heart Assoc. 2019;8:e010874 pubmed publisher
Netsrithong R, Promnakhon N, Boonkaew B, Vatanashevanopakorn C, Pattanapanyasat K, Wattanapanitch M. Generation of two induced pluripotent stem cell lines (MUSIi011-A and MUSIi011-B) from peripheral blood T lymphocytes of a healthy individual. Stem Cell Res. 2019;39:101487 pubmed publisher
Ray R, Maritz M, Avenant C, Tomasicchio M, Dlamini S, Van der Spuy Z, et al. The contraceptive medroxyprogesterone acetate, unlike norethisterone, directly increases R5 HIV-1 infection in human cervical explant tissue at physiologically relevant concentrations. Sci Rep. 2019;9:4334 pubmed publisher
Bak S, Tischer S, Dragon A, Ravens S, Pape L, Koenecke C, et al. Selective Effects of mTOR Inhibitor Sirolimus on Naïve and CMV-Specific T Cells Extending Its Applicable Range Beyond Immunosuppression. Front Immunol. 2018;9:2953 pubmed publisher
Collins P, Cella M, Porter S, Li S, Gurewitz G, Hong H, et al. Gene Regulatory Programs Conferring Phenotypic Identities to Human NK Cells. Cell. 2019;176:348-360.e12 pubmed publisher
Johnson D, Taabazuing C, Okondo M, Chui A, Rao S, Brown F, et al. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Nat Med. 2018;24:1151-1156 pubmed publisher
Setliff I, McDonnell W, Raju N, Bombardi R, Murji A, Scheepers C, et al. Multi-Donor Longitudinal Antibody Repertoire Sequencing Reveals the Existence of Public Antibody Clonotypes in HIV-1 Infection. Cell Host Microbe. 2018;23:845-854.e6 pubmed publisher
SAJADI M, Dashti A, Rikhtegaran Tehrani Z, Tolbert W, Seaman M, Ouyang X, et al. Identification of Near-Pan-neutralizing Antibodies against HIV-1 by Deconvolution of Plasma Humoral Responses. Cell. 2018;173:1783-1795.e14 pubmed publisher
Maritz M, Ray R, Bick A, Tomasicchio M, Woodland J, Govender Y, et al. Medroxyprogesterone acetate, unlike norethisterone, increases HIV-1 replication in human peripheral blood mononuclear cells and an indicator cell line, via mechanisms involving the glucocorticoid receptor, increased CD4/CD8 ratios and CCR5 levels. PLoS ONE. 2018;13:e0196043 pubmed publisher
Chen L, Tian Y, Zhan K, Chen A, Weng Z, Huang J, et al. A humanized TCR retaining authentic specificity and affinity conferred potent anti-tumour cytotoxicity. Immunology. 2018;155:123-136 pubmed publisher
Yuan Z, Kang G, Daharsh L, Fan W, Li Q. SIVcpz closely related to the ancestral HIV-1 is less or non-pathogenic to humans in a hu-BLT mouse model. Emerg Microbes Infect. 2018;7:59 pubmed publisher
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product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300405
Clone :
UCHT1
Item :
FITC anti-human CD3
Isotype :
Mouse IgG1, κ
Other Names :
T3, CD3ε
Size :
25 tests
Price (USD) :
22 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections^11, immunoprecipitation^1, activation of T cells^2,3,5, Western blotting^9, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA