product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD3
catalog :
300332
quantity :
1 mg
price :
396 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HIT3a
reactivity :
human, mouse, chimpanzee
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 58
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig s1
Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed publisher
  • flow cytometry; human; loading ...; fig 1f, s1b
Wu X, Xia T, Shin W, Yu K, Jung W, Herrmann A, et al. Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8. MBio. 2022;13:e0040222 pubmed publisher
  • flow cytometry; human; loading ...
Horiuchi S, Wu H, Liu W, Schmitt N, Provot J, Liu Y, et al. Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells. Sci Adv. 2021;7:eabj1249 pubmed publisher
  • flow cytometry; human; loading ...; fig 2
Martínez Zamudio R, Dewald H, Vasilopoulos T, Gittens Williams L, Fitzgerald Bocarsly P, Herbig U. Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans. Aging Cell. 2021;20:e13344 pubmed publisher
  • flow cytometry; human; loading ...
Vavassori V, Mercuri E, Marcovecchio G, Castiello M, Schiroli G, Albano L, et al. Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome. EMBO Mol Med. 2021;13:e13545 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Deeba E, Lambrianides A, Pantzaris M, Krashias G, Christodoulou C. The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients. BMC Res Notes. 2020;13:460 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e9i
Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed publisher
  • flow cytometry; human; loading ...; fig s14b
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 7d, 7f
Rasoulouniriana D, Santana Magal N, Gutwillig A, Farhat Younis L, Wine Y, Saperia C, et al. A distinct subset of FcγRI-expressing Th1 cells exert antibody-mediated cytotoxic activity. J Clin Invest. 2019;129:4151-4164 pubmed publisher
  • flow cytometry; human; loading ...; fig s8
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
  • flow cytometry; human; loading ...; fig s5
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
  • flow cytometry; human; 1:100; loading ...
Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Inoue Y, Endo S, Matsuno N, Kikukawa Y, Shichijo T, Koga K, et al. Safety of mogamulizumab for relapsed ATL after allogeneic hematopoietic cell transplantation. Bone Marrow Transplant. 2018;: pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 1a
Fischer M, Bantug G, Dimeloe S, Gubser P, Burgener A, Grählert J, et al. Early effector maturation of naïve human CD8+ T cells requires mitochondrial biogenesis. Eur J Immunol. 2018;48:1632-1643 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Pugh J, Nemat Gorgani N, Norman P, Guethlein L, Parham P. Human NK Cells Downregulate Zap70 and Syk in Response to Prolonged Activation or DNA Damage. J Immunol. 2018;200:1146-1158 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Serr I, Fürst R, Ott V, Scherm M, Nikolaev A, Gökmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
  • immunocytochemistry; human; loading ...; fig s9
Jung Y, Riven I, Feigelson S, Kartvelishvily E, Tohya K, Miyasaka M, et al. Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies. Proc Natl Acad Sci U S A. 2016;113:E5916-E5924 pubmed
  • flow cytometry; human; fig 1
Mercer F, Diala F, Chen Y, Molgora B, Ng S, Johnson P. Leukocyte Lysis and Cytokine Induction by the Human Sexually Transmitted Parasite Trichomonas vaginalis. PLoS Negl Trop Dis. 2016;10:e0004913 pubmed publisher
  • blocking or activating experiments; human; loading ...
Ma Q, Garber H, Lu S, He H, Tallis E, Ding X, et al. A novel TCR-like CAR with specificity for PR1/HLA-A2 effectively targets myeloid leukemia in vitro when expressed in human adult peripheral blood and cord blood T cells. Cytotherapy. 2016;18:985-94 pubmed publisher
  • flow cytometry; human; fig 2
Yu C, Liu Y, Chan J, Tong J, Li Z, Shi M, et al. Identification of human plasma cells with a lamprey monoclonal antibody. JCI Insight. 2016;1: pubmed
  • flow cytometry; human; loading ...; fig 1b
Malkiel S, Jeganathan V, Wolfson S, Manjarrez Orduno N, Marasco E, Aranow C, et al. Checkpoints for Autoreactive B Cells in the Peripheral Blood of Lupus Patients Assessed by Flow Cytometry. Arthritis Rheumatol. 2016;68:2210-20 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
James E, Gates T, LaFond R, Yamamoto S, Ni C, Mai D, et al. Neuroinvasive West Nile Infection Elicits Elevated and Atypically Polarized T Cell Responses That Promote a Pathogenic Outcome. PLoS Pathog. 2016;12:e1005375 pubmed publisher
  • flow cytometry; human
Zhu F, Qiao J, Cao J, Sun H, Wu Q, Sun Z, et al. Decreased level of cytotoxic T lymphocyte antigen-4 (CTLA-4) in patients with acute immune thrombocytopenia (ITP). Thromb Res. 2015;136:797-802 pubmed publisher
  • flow cytometry; human
Li Z, Li W, Li N, Jiao Y, Chen D, Cui L, et al. γδ T cells are involved in acute HIV infection and associated with AIDS progression. PLoS ONE. 2014;9:e106064 pubmed publisher
  • flow cytometry; human
Foster A, Baliwag J, Chen C, Guzman A, Stoll S, Gudjonsson J, et al. IL-36 promotes myeloid cell infiltration, activation, and inflammatory activity in skin. J Immunol. 2014;192:6053-61 pubmed publisher
  • blocking or activating experiments; chimpanzee
Hodara V, Parodi L, Chavez D, Smith L, Lanford R, Giavedoni L. Characterization of ??T cells in naïve and HIV-infected chimpanzees and their responses to T-cell activators in vitro. J Med Primatol. 2014;43:258-71 pubmed publisher
Wang C, Li Y, Jia L, Kim J, Li J, Deng P, et al. CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance. Cell Stem Cell. 2021;28:1597-1613.e7 pubmed publisher
Kalafati L, Kourtzelis I, Schulte Schrepping J, Li X, Hatzioannou A, Grinenko T, et al. Innate Immune Training of Granulopoiesis Promotes Anti-tumor Activity. Cell. 2020;183:771-785.e12 pubmed publisher
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
Eken A, Okus Z, Erdem S, Azizoglu Z, Haliloglu Y, Bicer A, et al. IL-15 negatively regulates curdlan-induced IL-23 production by human monocyte-derived dendritic cells and subsequent Th17 response. North Clin Istanb. 2019;6:379-387 pubmed publisher
Farmer J, Allard Chamard H, Sun N, Ahmad M, Bertocchi A, Mahajan V, et al. Induction of metabolic quiescence defines the transitional to follicular B cell switch. Sci Signal. 2019;12: pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Matsuda Lennikov M, Biancalana M, Zou J, Ravell J, Zheng L, Kanellopoulou C, et al. Magnesium transporter 1 (MAGT1) deficiency causes selective defects in N-linked glycosylation and expression of immune-response genes. J Biol Chem. 2019;294:13638-13656 pubmed publisher
Bruner K, Wang Z, Simonetti F, Bender A, Kwon K, Sengupta S, et al. A quantitative approach for measuring the reservoir of latent HIV-1 proviruses. Nature. 2019;566:120-125 pubmed publisher
Franco L, Gadkari M, Howe K, Sun J, Kardava L, Kumar P, et al. Immune regulation by glucocorticoids can be linked to cell type-dependent transcriptional responses. J Exp Med. 2019;216:384-406 pubmed publisher
Neal J, Li X, Zhu J, Giangarrà V, Grzeskowiak C, Ju J, et al. Organoid Modeling of the Tumor Immune Microenvironment. Cell. 2018;175:1972-1988.e16 pubmed publisher
Kim C, Hu B, Jadhav R, Jin J, Zhang H, Cavanagh M, et al. Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells. Cell Rep. 2018;25:2148-2162.e5 pubmed publisher
Rataj F, Jacobi S, Stoiber S, Asang F, Ogonek J, Tokarew N, et al. High-affinity CD16-polymorphism and Fc-engineered antibodies enable activity of CD16-chimeric antigen receptor-modified T cells for cancer therapy. Br J Cancer. 2019;120:79-87 pubmed publisher
Pan G, Cao X, Liu B, Li C, Li D, Zheng J, et al. OSBP-related protein 4L promotes phospholipase Cβ3 translocation from the nucleus to the plasma membrane in Jurkat T-cells. J Biol Chem. 2018;293:17430-17441 pubmed publisher
Theodoraki M, Hoffmann T, Jackson E, Whiteside T. Exosomes in HNSCC plasma as surrogate markers of tumour progression and immune competence. Clin Exp Immunol. 2018;194:67-78 pubmed publisher
Achita P, Dervović D, Ly D, Lee J, Haug T, Joe B, et al. Infusion of ex-vivo expanded human TCR-αβ+ double-negative regulatory T cells delays onset of xenogeneic graft-versus-host disease. Clin Exp Immunol. 2018;193:386-399 pubmed publisher
Theodoraki M, Hoffmann T, Whiteside T. Separation of plasma-derived exosomes into CD3(+) and CD3(-) fractions allows for association of immune cell and tumour cell markers with disease activity in HNSCC patients. Clin Exp Immunol. 2018;192:271-283 pubmed publisher
Böiers C, Richardson S, Laycock E, Zriwil A, Turati V, Brown J, et al. A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1. Dev Cell. 2018;44:362-377.e7 pubmed publisher
Sato K, Misawa N, Takeuchi J, Kobayashi T, Izumi T, Aso H, et al. Experimental Adaptive Evolution of Simian Immunodeficiency Virus SIVcpz to Pandemic Human Immunodeficiency Virus Type 1 by Using a Humanized Mouse Model. J Virol. 2018;92: pubmed publisher
Pinder C, Kratochvil S, Cizmeci D, Muir L, Guo Y, Shattock R, et al. Isolation and Characterization of Antigen-Specific Plasmablasts Using a Novel Flow Cytometry-Based Ig Capture Assay. J Immunol. 2017;199:4180-4188 pubmed publisher
Marras F, Casabianca A, Bozzano F, Ascierto M, Orlandi C, Di Biagio A, et al. Control of the HIV-1 DNA Reservoir Is Associated In Vivo and In Vitro with NKp46/NKp30 (CD335 CD337) Inducibility and Interferon Gamma Production by Transcriptionally Unique NK Cells. J Virol. 2017;91: pubmed publisher
Chen X, Poncette L, Blankenstein T. Human TCR-MHC coevolution after divergence from mice includes increased nontemplate-encoded CDR3 diversity. J Exp Med. 2017;214:3417-3433 pubmed publisher
Ott P, Hu Z, Keskin D, Shukla S, Sun J, Bozym D, et al. An immunogenic personal neoantigen vaccine for patients with melanoma. Nature. 2017;547:217-221 pubmed publisher
Patel A, Zhang Y, Fullerton J, Boelen L, Rongvaux A, Maini A, et al. The fate and lifespan of human monocyte subsets in steady state and systemic inflammation. J Exp Med. 2017;214:1913-1923 pubmed publisher
Mujib S, Liu J, Rahman A, Schwartz J, Bonner P, Yue F, et al. Comprehensive Cross-Clade Characterization of Antibody-Mediated Recognition, Complement-Mediated Lysis, and Cell-Mediated Cytotoxicity of HIV-1 Envelope-Specific Antibodies toward Eradication of the HIV-1 Reservoir. J Virol. 2017;91: pubmed publisher
Costa N, Marques O, Godinho S, Carvalho C, Leal B, Figueiredo A, et al. Two separate effects contribute to regulatory T cell defect in systemic lupus erythematosus patients and their unaffected relatives. Clin Exp Immunol. 2017;189:318-330 pubmed publisher
Abolhassani H, Edwards E, Ikinciogullari A, Jing H, Borte S, Buggert M, et al. Combined immunodeficiency and Epstein-Barr virus-induced B cell malignancy in humans with inherited CD70 deficiency. J Exp Med. 2017;214:91-106 pubmed publisher
Grier A, Burleigh S, Sahni J, Clough C, Cardot V, Choe D, et al. pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences. Mol Ther Nucleic Acids. 2016;5:e306 pubmed publisher
Islas Vazquez L, Prado Garcia H, Aguilar Cazares D, Meneses Flores M, Galicia Velasco M, Romero Garcia S, et al. LAP TGF-Beta Subset of CD4(+)CD25(+)CD127(-) Treg Cells is Increased and Overexpresses LAP TGF-Beta in Lung Adenocarcinoma Patients. Biomed Res Int. 2015;2015:430943 pubmed publisher
Selvan S, Dowling J. "Adherent" versus Other Isolation Strategies for Expanding Purified, Potent, and Activated Human NK Cells for Cancer Immunotherapy. Biomed Res Int. 2015;2015:869547 pubmed publisher
Rancan C, Schirrmann L, Hüls C, Zeidler R, Moosmann A. Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells. PLoS Pathog. 2015;11:e1004906 pubmed publisher
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300332
Clone :
HIT3a
Item :
Ultra-LEAF™ Purified anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
1 mg
Price (USD) :
396 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Application Notes :
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes^4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA