product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-human CD3
catalog :
300318
quantity :
100 tests
price :
311 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
HIT3a
reactivity :
human
application :
flow cytometry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100
Wang Q, Gao H, Clark K, Mugisha C, Davis K, Tang J, et al. CARD8 is an inflammasome sensor for HIV-1 protease activity. Science. 2021;371: pubmed publisher
  • flow cytometry; human; loading ...; fig s5
Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
  • flow cytometry; human; loading ...; fig 2c
Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Kaczmarek D, Kokordelis P, Kramer B, Glässner A, Wolter F, Goeser F, et al. Alterations of the NK cell pool in HIV/HCV co-infection. PLoS ONE. 2017;12:e0174465 pubmed publisher
  • flow cytometry; human; loading ...
Flint S, Gibson A, Lucas G, Nandigam R, Taylor L, Provan D, et al. A distinct plasmablast and naïve B-cell phenotype in primary immune thrombocytopenia. Haematologica. 2016;101:698-706 pubmed publisher
Feng Y, Zhang Y, He Z, Huang H, Tian X, Wang G, et al. Immunogenicity of an inactivated SARS-CoV-2 vaccine in people living with HIV-1: a non-randomized cohort study. EClinicalMedicine. 2022;43:101226 pubmed publisher
Beatson R, Parente Pereira A, Halim L, Cozzetto D, Hull C, Whilding L, et al. TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer. Cell Rep Med. 2021;2:100473 pubmed publisher
Kreutmair S, Unger S, Núñez N, Ingelfinger F, Alberti C, De Feo D, et al. Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia. Immunity. 2021;54:1578-1593.e5 pubmed publisher
Harb H, Benamar M, Lai P, Contini P, Griffith J, Crestani E, et al. Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections. Immunity. 2021;54:1186-1199.e7 pubmed publisher
Hearnden R, Sandhar B, Vyas V, Longhi M. Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications. STAR Protoc. 2021;2:100422 pubmed publisher
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
Wang F, Trier A, Li F, Kim S, Chen Z, Chai J, et al. A basophil-neuronal axis promotes itch. Cell. 2021;184:422-440.e17 pubmed publisher
Koh W, Zayats R, López P, Murooka T. Visualizing Cellular Dynamics and Protein Localization in 3D Collagen. STAR Protoc. 2020;1:100203 pubmed publisher
Ping Y, Li F, Nan S, Zhang D, Shi X, Shan J, et al. Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv. Front Cell Dev Biol. 2020;8:803 pubmed publisher
Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
Nixon C, Mavigner M, Sampey G, Brooks A, Spagnuolo R, Irlbeck D, et al. Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature. 2020;578:160-165 pubmed publisher
Kim D, Kobayashi T, Voisin B, Jo J, Sakamoto K, Jin S, et al. Targeted therapy guided by single-cell transcriptomic analysis in drug-induced hypersensitivity syndrome: a case report. Nat Med. 2020;26:236-243 pubmed publisher
Hagan T, Cortese M, Rouphael N, Boudreau C, Linde C, Maddur M, et al. Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans. Cell. 2019;178:1313-1328.e13 pubmed publisher
Friedensohn S, Lindner J, Cornacchione V, Iazeolla M, Miho E, Zingg A, et al. Synthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells. Front Immunol. 2018;9:1401 pubmed publisher
Neff C, Krueger O, Xiong K, Arif S, Nusbacher N, Schneider J, et al. Fecal Microbiota Composition Drives Immune Activation in HIV-infected Individuals. EBioMedicine. 2018;30:192-202 pubmed publisher
Howson L, Napolitani G, Shepherd D, Ghadbane H, Kurupati P, Preciado Llanes L, et al. MAIT cell clonal expansion and TCR repertoire shaping in human volunteers challenged with Salmonella Paratyphi A. Nat Commun. 2018;9:253 pubmed publisher
Gladkikh A, Potashnikova D, Tatarskiy V, Yastrebova M, Khamidullina A, Barteneva N, et al. Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70. Cancer Med. 2017;6:2984-2997 pubmed publisher
Nakano Y, Misawa N, Juarez Fernandez G, Moriwaki M, Nakaoka S, Funo T, et al. HIV-1 competition experiments in humanized mice show that APOBEC3H imposes selective pressure and promotes virus adaptation. PLoS Pathog. 2017;13:e1006348 pubmed publisher
Palamides P, Jodeleit H, Föhlinger M, Beigel F, Herbach N, Mueller T, et al. A mouse model for ulcerative colitis based on NOD-scid IL2R ?null mice reconstituted with peripheral blood mononuclear cells from affected individuals. Dis Model Mech. 2016;9:985-97 pubmed publisher
Gerstner S, Kohler W, Heidkamp G, Purbojo A, Uchida S, Ekici A, et al. Specific phenotype and function of CD56-expressing innate immune cell subsets in human thymus. J Leukoc Biol. 2016;100:1297-1310 pubmed
Younis R, Han K, Webb T. Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells. J Immunol. 2016;196:1419-29 pubmed publisher
Bradley S, Suárez Fueyo A, Moss D, Kyttaris V, Tsokos G. T Cell Transcriptomes Describe Patient Subtypes in Systemic Lupus Erythematosus. PLoS ONE. 2015;10:e0141171 pubmed publisher
Li Causi E, Parikh S, Chudley L, Layfield D, Ottensmeier C, Stevenson F, et al. Vaccination Expands Antigen-Specific CD4+ Memory T Cells and Mobilizes Bystander Central Memory T Cells. PLoS ONE. 2015;10:e0136717 pubmed publisher
Liu Y, Bezverbnaya K, Zhao T, Parsons M, Shi M, Treanor B, et al. Involvement of the HCK and FGR src-family kinases in FCRL4-mediated immune regulation. J Immunol. 2015;194:5851-60 pubmed publisher
Sato K, Takeuchi J, Misawa N, Izumi T, Kobayashi T, Kimura Y, et al. APOBEC3D and APOBEC3F potently promote HIV-1 diversification and evolution in humanized mouse model. PLoS Pathog. 2014;10:e1004453 pubmed publisher
Luetke Eversloh M, Hammer Q, Durek P, Nordström K, Gasparoni G, Pink M, et al. Human cytomegalovirus drives epigenetic imprinting of the IFNG locus in NKG2Chi natural killer cells. PLoS Pathog. 2014;10:e1004441 pubmed publisher
Ohue Y, Kurose K, Mizote Y, Matsumoto H, Nishio Y, Isobe M, et al. Prolongation of overall survival in advanced lung adenocarcinoma patients with the XAGE1 (GAGED2a) antibody. Clin Cancer Res. 2014;20:5052-63 pubmed publisher
Hinderer S, Schesny M, Bayrak A, Ibold B, Hampel M, Walles T, et al. Engineering of fibrillar decorin matrices for a tissue-engineered trachea. Biomaterials. 2012;33:5259-66 pubmed publisher
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300318
Clone :
HIT3a
Item :
APC/Cyanine7 anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
311 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Application Notes :
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes^4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA