PE/Cyanine5 anti-human CD3 | BioLegend 300310 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine5 anti-human CD3

catalog :

300310

quantity :

100 tests

price :

280 USD

clonality :

monoclonal

host :

mouse

conjugate :

PE-Cy5

clone name :

HIT3a

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; tbl s1	Chang C, Hale S, Cox C, Blair A, Kronsteiner B, Grabowska R, et al. Junctional Adhesion Molecule-A Is Highly Expressed on Human Hematopoietic Repopulating Cells and Associates with the Key Hematopoietic Chemokine Receptor CXCR4. Stem Cells. 2016;34:1664-78 pubmed publisher
flow cytometry; human; loading ...; fig 2a	James E, Gates T, LaFond R, Yamamoto S, Ni C, Mai D, et al. Neuroinvasive West Nile Infection Elicits Elevated and Atypically Polarized T Cell Responses That Promote a Pathogenic Outcome. PLoS Pathog. 2016;12:e1005375 pubmed publisher
	Gupta R, Turati V, Brian D, Thrussel C, Wilbourn B, May G, et al. Nov/CCN3 Enhances Cord Blood Engraftment by Rapidly Recruiting Latent Human Stem Cell Activity. Cell Stem Cell. 2020;26:527-541.e8 pubmed publisher
	Labuhn M, Perkins K, Matzk S, Varghese L, Garnett C, Papaemmanuil E, et al. Mechanisms of Progression of Myeloid Preleukemia to Transformed Myeloid Leukemia in Children with Down Syndrome. Cancer Cell. 2019;36:123-138.e10 pubmed publisher
	Rodriguez Meira A, Buck G, Clark S, Povinelli B, Alcolea V, Louka E, et al. Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing. Mol Cell. 2019;73:1292-1305.e8 pubmed publisher
	Barruet E, Morales B, Cain C, Ton A, Wentworth K, Chan T, et al. NF-κB/MAPK activation underlies ACVR1-mediated inflammation in human heterotopic ossification. JCI Insight. 2018;3: pubmed publisher
	Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
	Bill M, B van Kooten Niekerk P, S Woll P, Laine Herborg L, Stidsholt Roug A, Hokland P, et al. Mapping the CLEC12A expression on myeloid progenitors in normal bone marrow; implications for understanding CLEC12A-related cancer stem cell biology. J Cell Mol Med. 2018;22:2311-2318 pubmed publisher
	Böiers C, Richardson S, Laycock E, Zriwil A, Turati V, Brown J, et al. A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1. Dev Cell. 2018;44:362-377.e7 pubmed publisher
	Ho T, LaMere M, Stevens B, Ashton J, Myers J, O Dwyer K, et al. Evolution of acute myelogenous leukemia stem cell properties after treatment and progression. Blood. 2016;128:1671-8 pubmed publisher
	Quast I, Cueni F, Nimmerjahn F, Tackenberg B, Lunemann J. Deregulated Fcγ receptor expression in patients with CIDP. Neurol Neuroimmunol Neuroinflamm. 2015;2:e148 pubmed publisher
	Chow I, Yang J, Gates T, James E, Mai D, Greenbaum C, et al. Assessment of CD4+ T cell responses to glutamic acid decarboxylase 65 using DQ8 tetramers reveals a pathogenic role of GAD65 121-140 and GAD65 250-266 in T1D development. PLoS ONE. 2014;9:e112882 pubmed publisher

product information

Antigen :

CD3

Apps. Abbrev. :

Cat # :

300310

Clone :

HIT3a

Item :

PE/Cyanine5 anti-human CD3

Isotype :

Mouse IgG2a, κ

Other Names :

T3, CD3ε

Size :

100 tests

Price (USD) :

280 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_CYANINE5

Application Notes :

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes^4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;

more info or order :

BioLegend product webpage