product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human CD3
catalog :
300305
quantity :
25 tests
price :
22 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
HIT3a
reactivity :
human
application :
flow cytometry
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 6b
Liu Y, Wang L, Song Q, Ali M, Crowe W, Kucera G, et al. Intrapleural nano-immunotherapy promotes innate and adaptive immune responses to enhance anti-PD-L1 therapy for malignant pleural effusion. Nat Nanotechnol. 2022;17:206-216 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Deeba E, Lambrianides A, Pantzaris M, Krashias G, Christodoulou C. The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients. BMC Res Notes. 2020;13:460 pubmed publisher
  • flow cytometry; human; loading ...; fig s14b
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • flow cytometry; human; 1:100; loading ...
Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
  • flow cytometry; human; 1:100; fig 5
Parameswaran R, Ramakrishnan P, Moreton S, Xia Z, Hou Y, Lee D, et al. Repression of GSK3 restores NK cell cytotoxicity in AML patients. Nat Commun. 2016;7:11154 pubmed publisher
Huang C, Ren S, Chen Y, Liu A, Wu Q, Jiang T, et al. PD-L1 methylation restricts PD-L1/PD-1 interactions to control cancer immune surveillance. Sci Adv. 2023;9:eade4186 pubmed publisher
Wu X, Wang A, Zhang S, Wang X, Guo D, Zhu W, et al. Multiomic landscape of immune pathogenesis in Kimura's disease. iScience. 2023;26:106559 pubmed publisher
Tang C, Sun H, Kadoki M, Han W, Ye X, Makusheva Y, et al. Blocking Dectin-1 prevents colorectal tumorigenesis by suppressing prostaglandin E2 production in myeloid-derived suppressor cells and enhancing IL-22 binding protein expression. Nat Commun. 2023;14:1493 pubmed publisher
Nisa B, Nakanishi R, Tanaka M, Lin H, Hirabayashi T, Maeshige N, et al. Mild Hyperbaric Oxygen Exposure Enhances Peripheral Circulatory Natural Killer Cells in Healthy Young Women. Life (Basel). 2023;13: pubmed publisher
Vyasamneni R, Kohler V, Karki B, Mahimkar G, Esaulova E, McGee J, et al. A universal MHCII technology platform to characterize antigen-specific CD4+ T cells. Cell Rep Methods. 2023;3:100388 pubmed publisher
Sun L, Yu J, Zhang N, Wang Y, Qi J. M1 macrophages may be effective adjuvants for promoting Th‑17 differentiation in HBeAg positive hepatitis patients with ALT ≤2ULN. Mol Med Rep. 2023;27: pubmed publisher
Cui B, Chen X, Sun J, Li S, Zhou G, Sun L, et al. Dendritic cells originating exosomal miR-193b-3p induces regulatory T cells to alleviate liver transplant rejection. Int Immunopharmacol. 2023;114:109541 pubmed publisher
Chen L, Liu S, Adah D, Sun Q, Liang Z, Ho M, et al. Soluble programmed death ligand-1-induced immunosuppressive effects on chimeric antigen receptor-natural killer cells targeting Glypican-3 in hepatocellular carcinoma. Immunology. 2023;169:204-218 pubmed publisher
Xu Q, Muyayalo K, Zhang Y, Wang H, Lin X, Liao A. Altered vitamin D metabolism is involved in the dysregulation of γδT cell function and their crosstalk with trophoblasts in recurrent pregnancy loss. Am J Reprod Immunol. 2023;89:e13581 pubmed publisher
Wang P, Mo Z, Zhang Y, Guo C, Chikede T, Chen D, et al. Serum IL-5 levels predict HBsAg seroclearance in patients treated with Nucleos(t)ide analogues combined with pegylated interferon. Front Immunol. 2022;13:1104329 pubmed publisher
Zhang J, Hu Y, Yang J, Li W, Zhang M, Wang Q, et al. Non-viral, specifically targeted CAR-T cells achieve high safety and efficacy in B-NHL. Nature. 2022;609:369-374 pubmed publisher
Wang M, Wei Y, Li Y, Li H, Jin J, Lu Y, et al. Targeting breast cancer with a combination of DNT and LAG3 checkpoint blockage and its mechanism. Immun Inflamm Dis. 2022;10:e626 pubmed publisher
Imbrechts M, Maes W, Ampofo L, Van den Berghe N, Calcoen B, Van Looveren D, et al. Potent neutralizing anti-SARS-CoV-2 human antibodies cure infection with SARS-CoV-2 variants in hamster model. iScience. 2022;25:104705 pubmed publisher
Rosa T, Mendes M, Linhares N, Rodrigues T, Dias A, Leal Calvo T, et al. The Type I Interferon Pathway Is Upregulated in the Cutaneous Lesions and Blood of Multibacillary Leprosy Patients With Erythema Nodosum Leprosum. Front Med (Lausanne). 2022;9:899998 pubmed publisher
Wang H, Cao K, Liu S, Xu Y, Tang L. Tim-3 Expression Causes NK Cell Dysfunction in Type 2 Diabetes Patients. Front Immunol. 2022;13:852436 pubmed publisher
Walle T, Kraske J, Liao B, Lenoir B, Timke C, von Bohlen Und Halbach E, et al. Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8. Sci Adv. 2022;8:eabh4050 pubmed publisher
Wu H, Zhu R, Zheng B, Liao G, Wang F, Ding J, et al. Single-Cell Sequencing Reveals an Intrinsic Heterogeneity of the Preovulatory Follicular Microenvironment. Biomolecules. 2022;12: pubmed publisher
Du Y, Peng Q, Cheng D, Pan T, Sun W, Wang H, et al. Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells. Nat Commun. 2022;13:231 pubmed publisher
Li X, Zhu Y, Zhang X, An X, Weng M, Shi J, et al. An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP. J Clin Invest. 2022;132: pubmed publisher
Wu G, Xu Y, Schultz R, Chen H, Xie J, Deng M, et al. LILRB3 supports acute myeloid leukemia development and regulates T-cell antitumor immune responses through the TRAF2-cFLIP-NF-κB signaling axis. Nat Cancer. 2021;2:1170-1184 pubmed publisher
Ding L, Yang Y, Ge Y, Lu Q, Yan Z, Chen X, et al. Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity. Cancers (Basel). 2021;13: pubmed publisher
Hu W, He M, Wang X, Sun Q, Kuang M. Specific CD8+ TCR Repertoire Recognizing Conserved Antigens of SARS-CoV-2 in Unexposed Population: A Prerequisite for Broad-Spectrum CD8+ T Cell Immunity. Vaccines (Basel). 2021;9: pubmed publisher
Gingrich A, Reiter T, Judge S, York D, Yanagisawa M, Razmara A, et al. Comparative Immunogenomics of Canine Natural Killer Cells as Immunotherapy Target. Front Immunol. 2021;12:670309 pubmed publisher
Wang X, Chen Y, Li Z, Huang B, Xu L, Lai J, et al. Single-Cell RNA-Seq of T Cells in B-ALL Patients Reveals an Exhausted Subset with Remarkable Heterogeneity. Adv Sci (Weinh). 2021;8:e2101447 pubmed publisher
Guo S, Huang C, Han F, Chen B, Ding Y, Zhao Y, et al. Gastric Cancer Mesenchymal Stem Cells Inhibit NK Cell Function through mTOR Signalling to Promote Tumour Growth. Stem Cells Int. 2021;2021:9989790 pubmed publisher
Delvecchio F, Fincham R, Spear S, Clear A, Roy Luzarraga M, Balkwill F, et al. Pancreatic Cancer Chemotherapy Is Potentiated by Induction of Tertiary Lymphoid Structures in Mice. Cell Mol Gastroenterol Hepatol. 2021;12:1543-1565 pubmed publisher
Pellegrini J, Martin C, Morelli M, Schander J, Tateosian N, Amiano N, et al. PGE2 displays immunosuppressive effects during human active tuberculosis. Sci Rep. 2021;11:13559 pubmed publisher
Turner J, O Halloran J, Kalaidina E, Kim W, Schmitz A, Zhou J, et al. SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses. Nature. 2021;596:109-113 pubmed publisher
Murdock B, Famie J, Piecuch C, Raue K, Mendelson F, Pieroni C, et al. NK cells associate with ALS in a sex- and age-dependent manner. JCI Insight. 2021;6: pubmed publisher
Grant R, Coopman K, Silva Gomes S, Campbell J, Kara B, Braybrook J, et al. Assessment of Protocol Impact on Subjectivity Uncertainty When Analyzing Peripheral Blood Mononuclear Cell Flow Cytometry Data Files. Methods Protoc. 2021;4: pubmed publisher
Sutton H, Aye R, Idris A, Vistein R, Nduati E, Kai O, et al. Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans. Cell Rep. 2021;34:108684 pubmed publisher
Jiang Q, Isquith J, Ladel L, Mark A, Holm F, Mason C, et al. Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution. Cell Rep. 2021;34:108670 pubmed publisher
Mondala P, Vora A, Zhou T, Lazzari E, Ladel L, Luo X, et al. Selective antisense oligonucleotide inhibition of human IRF4 prevents malignant myeloma regeneration via cell cycle disruption. Cell Stem Cell. 2021;28:623-636.e9 pubmed publisher
S xe1 nchez Vargas L, Hern xe1 ndez Flores K, Thomas Dupont P, Izaguirre Hern xe1 ndez I, S xe1 nchez Marce E, Remes Ruiz R, et al. Characterization of the IL-17 and CD4+ Th17 Cells in the Clinical Course of Dengue Virus Infections. Viruses. 2020;12: pubmed publisher
Poran A, Scherer J, Bushway M, Besada R, Balogh K, Wanamaker A, et al. Combined TCR Repertoire Profiles and Blood Cell Phenotypes Predict Melanoma Patient Response to Personalized Neoantigen Therapy plus Anti-PD-1. Cell Rep Med. 2020;1:100141 pubmed publisher
Yang J, Zhang J, Lu R, Tan Y, Du G, Zhou G. T cell-derived exosomes induced macrophage inflammatory protein-1α/β drive the trafficking of CD8+ T cells in oral lichen planus. J Cell Mol Med. 2020;24:14086-14098 pubmed publisher
Zhou Y, Bian S, Zhou X, Cui Y, Wang W, Wen L, et al. Single-Cell Multiomics Sequencing Reveals Prevalent Genomic Alterations in Tumor Stromal Cells of Human Colorectal Cancer. Cancer Cell. 2020;38:818-828.e5 pubmed publisher
Ott P, Hu Lieskovan S, Chmielowski B, Govindan R, Naing A, Bhardwaj N, et al. A Phase Ib Trial of Personalized Neoantigen Therapy Plus Anti-PD-1 in Patients with Advanced Melanoma, Non-small Cell Lung Cancer, or Bladder Cancer. Cell. 2020;183:347-362.e24 pubmed publisher
Dinh H, Eggert T, Meyer M, Zhu Y, Olingy C, Llewellyn R, et al. Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow. Immunity. 2020;53:319-334.e6 pubmed publisher
Liu Y, Tang L, Gao N, Diao Y, Zhong J, Deng Y, et al. Synthetic MUC1 breast cancer vaccine containing a Toll-like receptor 7 agonist exerts antitumor effects. Oncol Lett. 2020;20:2369-2377 pubmed publisher
Elias S, Kol I, Kahlon S, Amore R, Zeibak M, Mevorach D, et al. Anti-RhD antibody therapy modulates human natural killer cell function. Haematologica. 2021;106:1846-1856 pubmed publisher
Karlsson J, Nilsson L, Mitra S, Alsén S, Shelke G, Sah V, et al. Molecular profiling of driver events in metastatic uveal melanoma. Nat Commun. 2020;11:1894 pubmed publisher
Wadley A, Cullen T, Vautrinot J, Keane G, Bishop N, Coles S. High intensity interval exercise increases the frequency of peripheral PD-1+ CD8+ central memory T-cells and soluble PD-L1 in humans. Brain Behav Immun Health. 2020;3:100049 pubmed publisher
Bourges C, Groff A, Burren O, Gerhardinger C, Mattioli K, Hutchinson A, et al. Resolving mechanisms of immune-mediated disease in primary CD4 T cells. EMBO Mol Med. 2020;12:e12112 pubmed publisher
Omer O, Powell N, Lord G. Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease. Methods Mol Biol. 2020;2121:199-211 pubmed publisher
Dean J, Peters L, Fuhrman C, Seay H, Posgai A, Stimpson S, et al. Innate inflammation drives NK cell activation to impair Treg activity. J Autoimmun. 2020;108:102417 pubmed publisher
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product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
300305
Clone :
HIT3a
Item :
FITC anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
25 tests
Price (USD) :
22 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Application Notes :
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes^4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA