product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-MU/HU GL7 Antigen (T/B Cell Act. Marker)
catalog :
144606
quantity :
100 μg
price :
265 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
GL7
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 63
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1o
Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 9a
  • flow cytometry; mouse; 1:200; loading ...; fig s35a
Satofuka H, Abe S, Moriwaki T, Okada A, Kazuki K, Tanaka H, et al. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci. Nat Commun. 2022;13:1841 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1j
Kim E, Attia Z, Woodfint R, Zeng C, Kim S, Steiner H, et al. Inhibition of elastase enhances the adjuvanticity of alum and promotes anti-SARS-CoV-2 systemic and mucosal immunity. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 1l
Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4f
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Zhang Q, Xiang L, Zaman M, Dong W, He G, Deng G. Predominant Role of Immunoglobulin G in the Pathogenesis of Splenomegaly in Murine Lupus. Front Immunol. 2019;10:3020 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
  • flow cytometry; mouse; 1:120; loading ...; fig 1a
Koike T, Harada K, Horiuchi S, Kitamura D. The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2a
Grootjans J, Krupka N, Hosomi S, Matute J, Hanley T, Saveljeva S, et al. Epithelial endoplasmic reticulum stress orchestrates a protective IgA response. Science. 2019;363:993-998 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3b
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3
  • flow cytometry; mouse; loading ...; fig 1b
Tan H, Jegaskanda S, Juno J, Esterbauer R, Wong J, Kelly H, et al. Subdominance and poor intrinsic immunogenicity limit humoral immunity targeting influenza HA stem. J Clin Invest. 2019;129:850-862 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s7b
Theisen D, Davidson J, Briseño C, Gargaro M, Lauron E, Wang Q, et al. WDFY4 is required for cross-presentation in response to viral and tumor antigens. Science. 2018;362:694-699 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 3b
  • immunohistochemistry; mouse; loading ...; fig s3b
Rezende R, Lanser A, Rubino S, Kuhn C, Skillin N, Moreira T, et al. γδ T cells control humoral immune response by inducing T follicular helper cell differentiation. Nat Commun. 2018;9:3151 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4e
Tang C, Chang S, Paton A, Paton J, Gabrilovich D, Ploegh H, et al. Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization. J Cell Biol. 2018;217:1739-1755 pubmed publisher
  • flow cytometry; mouse; fig 2h
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Yazicioglu Y, Marin E, Sandhu C, Galiani S, Raza I, Ali M, et al. Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis. Nat Immunol. 2023;24:991-1006 pubmed publisher
Hao H, Wu S, Lin J, Zheng Z, Zhou Y, Zhang Y, et al. Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel. Nat Biomed Eng. 2023;7:928-942 pubmed publisher
Shi C, Zhang Q, Yao Y, Zeng F, Du C, Nijiati S, et al. Targeting the activity of T cells by membrane surface redox regulation for cancer theranostics. Nat Nanotechnol. 2023;18:86-97 pubmed publisher
Wu J, Yang K, Cai S, Zhang X, Hu L, Lin F, et al. A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation. Nat Commun. 2022;13:7321 pubmed publisher
Melzi E, Willis J, Ma K, Lin Y, Kratochvil S, Berndsen Z, et al. Membrane-bound mRNA immunogens lower the threshold to activate HIV Env V2 apex-directed broadly neutralizing B cell precursors in humanized mice. Immunity. 2022;55:2168-2186.e6 pubmed publisher
Zhu Y, Gu H, Yang L, Li N, Chen Q, Kang D, et al. Involvement of MST1/mTORC1/STAT1 activity in the regulation of B-cell receptor signalling by chemokine receptor 2. Clin Transl Med. 2022;12:e887 pubmed publisher
Hu Q, Xu T, Zhang M, Zhang H, Liu Y, Li H, et al. Diverging regulation of Bach2 protein and RNA expression determine cell fate in early B cell response. Cell Rep. 2022;40:111035 pubmed publisher
Gregoire C, Spinelli L, Villazala Merino S, Gil L, Holgado M, Moussa M, et al. Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism. Immunity. 2022;55:1216-1233.e9 pubmed publisher
Grenov A, Hezroni H, Lasman L, Hanna J, Shulman Z. YTHDF2 suppresses the plasmablast genetic program and promotes germinal center formation. Cell Rep. 2022;39:110778 pubmed publisher
MacLean A, Richmond N, Koneva L, Attar M, Medina C, Thornton E, et al. Secondary influenza challenge triggers resident memory B cell migration and rapid relocation to boost antibody secretion at infected sites. Immunity. 2022;55:718-733.e8 pubmed publisher
Stienne C, Virgen Slane R, Elm xe9 n L, Veny M, Huang S, Nguyen J, et al. Btla signaling in conventional and regulatory lymphocytes coordinately tempers humoral immunity in the intestinal mucosa. Cell Rep. 2022;38:110553 pubmed publisher
Kodali S, Li M, Budai M, Chen M, Wang J. Protection of Quiescence and Longevity of IgG Memory B Cells by Mitochondrial Autophagy. J Immunol. 2022;208:1085-1098 pubmed publisher
Chen J, Chow R, Song E, Mao T, Israelow B, Kamath K, et al. High-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cells. Sci Immunol. 2022;7:eabl5652 pubmed publisher
Mi Z, Zhao L, Sun M, Gao T, Wang Y, Sui B, et al. Overexpression of Interleukin-33 in Recombinant Rabies Virus Enhances Innate and Humoral Immune Responses through Activation of Dendritic Cell-Germinal Center Reactions. Vaccines (Basel). 2021;10: pubmed publisher
Ullrich L, Lueder Y, Juergens A, Wilharm A, Barros Martins J, Bubke A, et al. IL-4-Producing Vγ1+/Vδ6+ γδ T Cells Sustain Germinal Center Reactions in Peyer's Patches of Mice. Front Immunol. 2021;12:729607 pubmed publisher
Trindade B, Ceglia S, Berthelette A, Raso F, Howley K, Muppidi J, et al. The cholesterol metabolite 25-hydroxycholesterol restrains the transcriptional regulator SREBP2 and limits intestinal IgA plasma cell differentiation. Immunity. 2021;54:2273-2287.e6 pubmed publisher
Chen C, Zhang C, Li H, Wang Z, Yuan Y, Zhou M, et al. Toll-Like Receptor 4 Regulates Rabies Virus-Induced Humoral Immunity through Recruitment of Conventional Type 2 Dendritic Cells to Lymph Organs. J Virol. 2021;95:e0082921 pubmed publisher
Glaros V, Rauschmeier R, Artemov A, Reinhardt A, Ols S, Emmanouilidi A, et al. Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response. Immunity. 2021;54:2005-2023.e10 pubmed publisher
Wang Z, Yuan Y, Chen C, Zhang C, Huang F, Zhou M, et al. Colloidal Manganese Salt Improves the Efficacy of Rabies Vaccines in Mice, Cats, and Dogs. J Virol. 2021;95:e0141421 pubmed publisher
Chauveau L, Bridgeman A, Tan T, Beveridge R, Frost J, Rijal P, et al. Inclusion of cGAMP within virus-like particle vaccines enhances their immunogenicity. EMBO Rep. 2021;22:e52447 pubmed publisher
Zhao J, Zhang Y, Chen Y, Zhang J, Pei J, Cui M, et al. A novel oral rabies vaccine enhances the immunogenicity through increasing dendritic cells activation and germinal center formation by expressing U-OMP19 in a mouse model. Emerg Microbes Infect. 2021;10:913-928 pubmed publisher
Yang L, Li N, Yang D, Chen A, Tang J, Jing Y, et al. CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation. Cell Death Differ. 2021;: pubmed publisher
Gonzalez Figueroa P, Roco J, Papa I, Núñez Villacís L, Stanley M, Linterman M, et al. Follicular regulatory T cells produce neuritin to regulate B cells. Cell. 2021;184:1775-1789.e19 pubmed publisher
Alexandre Y, Devi S, Park S, Mackay L, Heath W, Mueller S. Systemic Inflammation Suppresses Lymphoid Tissue Remodeling and B Cell Immunity during Concomitant Local Infection. Cell Rep. 2020;33:108567 pubmed publisher
Wang X, Ray R, Kratochvil S, Melzi E, Lin Y, Giguere S, et al. Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptors. EMBO J. 2021;40:e105926 pubmed publisher
Du Z, Chen A, Huang L, Dai X, Chen Q, Yang D, et al. STAT3 couples with 14-3-3σ to regulate BCR signaling, B-cell differentiation, and IgE production. J Allergy Clin Immunol. 2021;147:1907-1923.e6 pubmed publisher
Hong J, Reynoso G, Andhey P, Swain A, Turner J, Boon A, et al. An Agonistic Anti-CD137 Antibody Disrupts Lymphoid Follicle Structure and T-Cell-Dependent Antibody Responses. Cell Rep Med. 2020;1: pubmed publisher
Zhang Y, Chen C, Deng C, Zhang C, Li N, Wang Z, et al. A novel rabies vaccine based on infectious propagating particles derived from hybrid VEEV-Rabies replicon. EBioMedicine. 2020;56:102819 pubmed publisher
Biram A, Winter E, Denton A, Zaretsky I, Dassa B, Bemark M, et al. B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches. Cell Rep. 2020;30:1910-1922.e5 pubmed publisher
Fallet B, Hao Y, Florova M, Cornille K, de Los Aires A, Girelli Zubani G, et al. Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses. Cell Rep. 2020;30:1013-1026.e7 pubmed publisher
Raju S, Verbaro D, Egawa T. PD-1 Signaling Promotes Control of Chronic Viral Infection by Restricting Type-I-Interferon-Mediated Tissue Damage. Cell Rep. 2019;29:2556-2564.e3 pubmed publisher
Jing Y, Kang D, Liu L, Huang H, Chen A, Yang L, et al. Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. J Allergy Clin Immunol. 2019;: pubmed publisher
Bidet K, Ho V, Chu C, Naim A, Thazin K, Chan K, et al. Mimicking immune signatures of flavivirus infection with targeted adjuvants improves dengue subunit vaccine immunogenicity. NPJ Vaccines. 2019;4:27 pubmed publisher
Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
Zanotti K, Maul R, Yang W, Gearhart P. DNA Breaks in Ig V Regions Are Predominantly Single Stranded and Are Generated by UNG and MSH6 DNA Repair Pathways. J Immunol. 2019;202:1573-1581 pubmed publisher
Woodruff M, Kim E, Luo W, Pulendran B. B Cell Competition for Restricted T Cell Help Suppresses Rare-Epitope Responses. Cell Rep. 2018;25:321-327.e3 pubmed publisher
Nagatake T, Suzuki H, Hirata S, Matsumoto N, Wada Y, Morimoto S, et al. Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice. Int Immunol. 2018;30:471-481 pubmed publisher
Rodda L, Lu E, Bennett M, Sokol C, Wang X, Luther S, et al. Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity. Immunity. 2018;48:1014-1028.e6 pubmed publisher
Ise W, Fujii K, Shiroguchi K, Ito A, Kometani K, Takeda K, et al. T Follicular Helper Cell-Germinal Center B Cell Interaction Strength Regulates Entry into Plasma Cell or Recycling Germinal Center Cell Fate. Immunity. 2018;48:702-715.e4 pubmed publisher
Castiblanco D, Maul R, Russell Knode L, Gearhart P. Co-Stimulation of BCR and Toll-Like Receptor 7 Increases Somatic Hypermutation, Memory B Cell Formation, and Secondary Antibody Response to Protein Antigen. Front Immunol. 2017;8:1833 pubmed publisher
Essig K, Hu D, Guimaraes J, Alterauge D, Edelmann S, Raj T, et al. Roquin Suppresses the PI3K-mTOR Signaling Pathway to Inhibit T Helper Cell Differentiation and Conversion of Treg to Tfr Cells. Immunity. 2017;47:1067-1082.e12 pubmed publisher
Zhu X, Yuan Z, Yan P, Li Y, Jiang H, Huang S. Effect of iRoot SP and mineral trioxide aggregate (MTA) on the viability and polarization of macrophages. Arch Oral Biol. 2017;80:27-33 pubmed publisher
Velazquez V, Meadows A, Pineda R, Camboni M, McCarty D, Fu H. Effective Depletion of Pre-existing Anti-AAV Antibodies Requires Broad Immune Targeting. Mol Ther Methods Clin Dev. 2017;4:159-168 pubmed publisher
Wu S, Majeed S, Evans T, Camus M, Wong N, Schollmeier Y, et al. Clathrin light chains' role in selective endocytosis influences antibody isotype switching. Proc Natl Acad Sci U S A. 2016;113:9816-21 pubmed publisher
Maul R, MacCarthy T, Frank E, Donigan K, McLenigan M, Yang W, et al. DNA polymerase ? functions in the generation of tandem mutations during somatic hypermutation of antibody genes. J Exp Med. 2016;213:1675-83 pubmed publisher
Kleiman E, Jia H, Loguercio S, Su A, Feeney A. YY1 plays an essential role at all stages of B-cell differentiation. Proc Natl Acad Sci U S A. 2016;113:E3911-20 pubmed publisher
product information
Antigen :
GL7
Apps. Abbrev. :
FC, IHC-F
Cat # :
144606
Clone :
GL7
Item :
Alexa Fluor® 647 anti-MU/HU GL7 Antigen (T/B Cell Act. Marker)
Isotype :
Rat IgM, κ
Other Names :
Ly77, T and B cell activation marker
Size :
100 μg
Price (USD) :
265 USD
Reactivity :
Mouse, Human
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
LPS activated DBA/J mouse B cells
Application Notes :
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA