product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse CD169 (Siglec-1)
catalog :
142407
quantity :
25 μg
price :
160 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
3D6.112
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 27
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 7c
Lin X, Twelkmeyer T, Zhu D, Zhang L, Zhao Y, Zhang C, et al. Homeostatic regulation of T follicular helper and antibody response to particle antigens by IL-1Ra of medullary sinus macrophage origin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2d
Freeman S, Uderhardt S, Saric A, Collins R, Buckley C, Mylvaganam S, et al. Lipid-gated monovalent ion fluxes regulate endocytic traffic and support immune surveillance. Science. 2020;367:301-305 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e6b
Solis A, Bielecki P, Steach H, Sharma L, Harman C, Yun S, et al. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature. 2019;573:69-74 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Zhang J, Supakorndej T, Krambs J, Rao M, Abou Ezzi G, Ye R, et al. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest. 2019;129:2920-2931 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3e
Uderhardt S, Martins A, Tsang J, Lämmermann T, Germain R. Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell. 2019;177:541-555.e17 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1c
Cao A, Alluqmani N, Buhari F, Wasim L, Smith L, Quaile A, et al. Galectin-9 binds IgM-BCR to regulate B cell signaling. Nat Commun. 2018;9:3288 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5c
Yanagihashi Y, Segawa K, Maeda R, Nabeshima Y, Nagata S. Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc Natl Acad Sci U S A. 2017;114:8800-8805 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5b
Turner V, Mabbott N. Structural and functional changes to lymph nodes in ageing mice. Immunology. 2017;151:239-247 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Le Gars M, Haustant M, Klezovich Bénard M, Paget C, Trottein F, Goossens P, et al. Mechanisms of Invariant NKT Cell Activity in Restraining Bacillus anthracis Systemic Dissemination. J Immunol. 2016;197:3225-3232 pubmed
Singh R, Choi B. A Transient Transfection-based Cell Adhesion Assay with 293T Cells. Bio Protoc. 2021;11:e3878 pubmed publisher
Gajdasik D, Gaspal F, Halford E, Fiancette R, Dutton E, Willis C, et al. Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues. Nat Commun. 2020;11:3421 pubmed publisher
Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart B, Brouilly N, et al. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages. Immunity. 2020;53:127-142.e7 pubmed publisher
Park C, Kehrl J. An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph node. elife. 2019;8: pubmed publisher
Broderick L, Yost S, Li D, McGeough M, Booshehri L, Guaderrama M, et al. Mutations in topoisomerase IIβ result in a B cell immunodeficiency. Nat Commun. 2019;10:3644 pubmed publisher
Rojo R, Raper A, Ozdemir D, Lefèvre L, Grabert K, Wollscheid Lengeling E, et al. Deletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations. Nat Commun. 2019;10:3215 pubmed publisher
Bidet K, Ho V, Chu C, Naim A, Thazin K, Chan K, et al. Mimicking immune signatures of flavivirus infection with targeted adjuvants improves dengue subunit vaccine immunogenicity. NPJ Vaccines. 2019;4:27 pubmed publisher
Pundir P, Liu R, Vasavda C, Serhan N, Limjunyawong N, Yee R, et al. A Connective Tissue Mast-Cell-Specific Receptor Detects Bacterial Quorum-Sensing Molecules and Mediates Antibacterial Immunity. Cell Host Microbe. 2019;26:114-122.e8 pubmed publisher
Farsakoglu Y, Palomino Segura M, Latino I, Zanaga S, Chatziandreou N, Pizzagalli D, et al. Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner. Cell Rep. 2019;26:2307-2315.e5 pubmed publisher
Uchil P, Pi R, Haugh K, Ladinsky M, Ventura J, Barrett B, et al. A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine Retrovirus. Cell Host Microbe. 2019;25:87-100.e10 pubmed publisher
Seydoux E, Liang H, Dubois Cauwelaert N, Archer M, Rintala N, Kramer R, et al. Effective Combination Adjuvants Engage Both TLR and Inflammasome Pathways To Promote Potent Adaptive Immune Responses. J Immunol. 2018;201:98-112 pubmed publisher
Vaartjes D, Nandakumar K, Holmdahl R, Raposo B. Increased salt exposure affects both lymphoid and myeloid effector functions, influencing innate-associated disease but not T-cell-associated autoimmunity. Immunology. 2018;: pubmed publisher
Baratin M, Simon L, Jorquera A, Ghigo C, Dembele D, Nowak J, et al. T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node. Immunity. 2017;47:349-362.e5 pubmed publisher
Wu X, Dayanand K, Thylur R, Norbury C, Gowda D. Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes. J Biol Chem. 2017;292:13615-13634 pubmed publisher
Deniset J, Surewaard B, Lee W, Kubes P. Splenic Ly6Ghigh mature and Ly6Gint immature neutrophils contribute to eradication of S. pneumoniae. J Exp Med. 2017;214:1333-1350 pubmed publisher
Sung S, Ge Y, Dai C, Wang H, Fu S, Sharma R, et al. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol. 2017;198:2589-2601 pubmed publisher
Ramos Perez W, Fang V, Escalante Alcalde D, Cammer M, Schwab S. A map of the distribution of sphingosine 1-phosphate in the spleen. Nat Immunol. 2015;16:1245-52 pubmed publisher
Klaas M, Oetke C, Lewis L, Erwig L, Heikema A, Easton A, et al. Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni. J Immunol. 2012;189:2414-22 pubmed publisher
product information
Antigen :
CD169
Apps. Abbrev. :
Flow Cytometry, IHC-F, 3D IHC
Cat # :
142407
Clone :
3D6.112
Item :
Alexa Fluor® 647 anti-mouse CD169 (Siglec-1)
Isotype :
Rat IgG2a, κ
Other Names :
Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)
Size :
25 μg
Price (USD) :
160 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
Purified Native Sialoadhesin from spleen
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections 1 , and spatial biology (IBEX) 4,5 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA