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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD64 (FcγRI)
catalog :
139304
quantity :
100 μg
price :
296 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
X54-5/7.1
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 76
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; fig 4d
Tran C, Scurr M, O Connor L, Buzzelli J, Ng G, Chin S, et al. IL-33 promotes gastric tumour growth in concert with activation and recruitment of inflammatory myeloid cells. Oncotarget. 2022;13:785-799 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1c
Tian N, Hu L, Lu Y, Tong L, Feng M, Liu Q, et al. TKT maintains intestinal ATP production and inhibits apoptosis-induced colitis. Cell Death Dis. 2021;12:853 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
Félix I, Jokela H, Karhula J, Kotaja N, Savontaus E, Salmi M, et al. Single-Cell Proteomics Reveals the Defined Heterogeneity of Resident Macrophages in White Adipose Tissue. Front Immunol. 2021;12:719979 pubmed publisher
  • flow cytometry; mouse; loading ...
Hering L, Katkeviciute E, Schwarzfischer M, Niechcial A, Riggs J, Wawrzyniak M, et al. Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Katano I, Ito R, Kawai K, Takahashi T. Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Front Immunol. 2020;11:532684 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2a
Luque Martin R, Van den Bossche J, Furze R, Neele A, van der Velden S, Gijbels M, et al. Targeting Histone Deacetylases in Myeloid Cells Inhibits Their Maturation and Inflammatory Function With Limited Effects on Atherosclerosis. Front Pharmacol. 2019;10:1242 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Joshi N, Watanabe S, Verma R, Jablonski R, Chen C, Cheresh P, et al. A spatially restricted fibrotic niche in pulmonary fibrosis is sustained by M-CSF/M-CSFR signalling in monocyte-derived alveolar macrophages. Eur Respir J. 2020;55: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Janela B, Patel A, Lau M, Goh C, Msallam R, Kong W, et al. A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils. Immunity. 2019;50:1069-1083.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19a
Normand S, Waldschmitt N, Neerincx A, Martinez Torres R, Chauvin C, Couturier Maillard A, et al. Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens. Nat Commun. 2018;9:5338 pubmed publisher
  • flow cytometry; mouse
Shin J, Lee S, Kim Y, Kim I, Kim Y, Kyeong D, et al. AHNAK deficiency promotes browning and lipolysis in mice via increased responsiveness to ?-adrenergic signalling. Sci Rep. 2016;6:23426 pubmed publisher
  • flow cytometry; mouse; fig s5
Wiesner D, Specht C, Lee C, Smith K, Mukaremera L, Lee S, et al. Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection. PLoS Pathog. 2015;11:e1004701 pubmed publisher
  • flow cytometry; mouse; fig 4
Karsten C, Laumonnier Y, Eurich B, Ender F, Bröker K, Roy S, et al. Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse. J Immunol. 2015;194:1841-55 pubmed publisher
Subramanian S, Busch C, Molawi K, Geirsdóttir L, Maurizio J, Vargas Aguilar S, et al. Long-term culture-expanded alveolar macrophages restore their full epigenetic identity after transfer in vivo. Nat Immunol. 2022;23:458-468 pubmed publisher
Afkhami S, D Agostino M, Zhang A, Stacey H, Marzok A, Kang A, et al. Respiratory mucosal delivery of next-generation COVID-19 vaccine provides robust protection against both ancestral and variant strains of SARS-CoV-2. Cell. 2022;185:896-915.e19 pubmed publisher
He J, Du Y, Li G, Xiao P, Sun X, Song W, et al. Myeloid Fbxw7 Prevents Pulmonary Fibrosis by Suppressing TGF-β Production. Front Immunol. 2021;12:760138 pubmed publisher
Guo L, Li N, Yang Z, Li H, Zheng H, Yang J, et al. Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection. Front Immunol. 2021;12:785457 pubmed publisher
Huangfu L, Cai X, Yang J, Wang H, Li Y, Dai Z, et al. Irisin attenuates inflammation in a mouse model of ulcerative colitis by altering the intestinal microbiota. Exp Ther Med. 2021;22:1433 pubmed publisher
MARCHELLETTA R, Krishnan M, Spalinger M, Placone T, Alvarez R, Sayoc Becerra A, et al. T cell protein tyrosine phosphatase protects intestinal barrier function by restricting epithelial tight junction remodeling. J Clin Invest. 2021;131: pubmed publisher
Nicolas Boluda A, Vaquero J, Vimeux L, Guilbert T, Barrin S, Kantari Mimoun C, et al. Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment. elife. 2021;10: pubmed publisher
Zhu B, Wu Y, Huang S, Zhang R, Son Y, Li C, et al. Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection. Immunity. 2021;54:1200-1218.e9 pubmed publisher
Brestoff J, Wilen C, Moley J, Li Y, Zou W, Malvin N, et al. Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity. Cell Metab. 2021;33:270-282.e8 pubmed publisher
Engler A, Ysasi A, Pihl R, Villacorta Martin C, Heston H, Richardson H, et al. Airway-Associated Macrophages in Homeostasis and Repair. Cell Rep. 2020;33:108553 pubmed publisher
Zhang X, Liu J, Wu L, Hu X. MicroRNAs of the miR-17~9 family maintain adipose tissue macrophage homeostasis by sustaining IL-10 expression. elife. 2020;9: pubmed publisher
Jiang Q, Maresch C, Petry S, Paradowska Dogan A, Bhushan S, Chang Y, et al. Elevated CCL2 causes Leydig cell malfunction in metabolic syndrome. JCI Insight. 2020;5: pubmed publisher
Hering L, Katkeviciute E, Schwarzfischer M, Busenhart P, Gottier C, Mrdjen D, et al. Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance. Front Immunol. 2020;11:1856 pubmed publisher
Runyan C, Welch L, Lecuona E, Shigemura M, Amarelle L, Abdala Valencia H, et al. Impaired phagocytic function in CX3CR1+ tissue-resident skeletal muscle macrophages prevents muscle recovery after influenza A virus-induced pneumonia in old mice. Aging Cell. 2020;: pubmed publisher
Oguri Y, Shinoda K, Kim H, Alba D, Bolus W, Wang Q, et al. CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell. 2020;: pubmed publisher
Utz S, See P, Mildenberger W, Thion M, Silvin A, Lutz M, et al. Early Fate Defines Microglia and Non-parenchymal Brain Macrophage Development. Cell. 2020;181:557-573.e18 pubmed publisher
Tkacz K, Rolski F, Czepiel M, Działo E, Siedlar M, Eriksson U, et al. Haploinsufficient Rock1+/- and Rock2+/- Mice Are Not Protected from Cardiac Inflammation and Postinflammatory Fibrosis in Experimental Autoimmune Myocarditis. Cells. 2020;9: pubmed publisher
Ferrer Font L, Mehta P, Harmos P, Schmidt A, Chappell S, Price K, et al. High-dimensional analysis of intestinal immune cells during helminth infection. elife. 2020;9: pubmed publisher
Pham T, Brewer S, Thurston T, Massis L, Honeycutt J, Lugo K, et al. Salmonella-Driven Polarization of Granuloma Macrophages Antagonizes TNF-Mediated Pathogen Restriction during Persistent Infection. Cell Host Microbe. 2020;27:54-67.e5 pubmed publisher
Wong E, Montoya B, Stotesbury C, Ferez M, Xu R, Sigal L. Langerhans Cells Orchestrate the Protective Antiviral Innate Immune Response in the Lymph Node. Cell Rep. 2019;29:3047-3059.e3 pubmed publisher
Mansouri N, Willis G, Fernandez Gonzalez A, Reis M, Nassiri S, Mitsialis S, et al. Mesenchymal stromal cell exosomes prevent and revert experimental pulmonary fibrosis through modulation of monocyte phenotypes. JCI Insight. 2019;4: pubmed publisher
Wu M, Liu J, Li F, Huang S, He J, Xue Y, et al. Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution. Mucosal Immunol. 2020;13:47-63 pubmed publisher
Swidergall M, Solis N, Wang Z, Phan Q, Marshall M, Lionakis M, et al. EphA2 Is a Neutrophil Receptor for Candida albicans that Stimulates Antifungal Activity during Oropharyngeal Infection. Cell Rep. 2019;28:423-433.e5 pubmed publisher
Ponzetta A, Carriero R, Carnevale S, Barbagallo M, Molgora M, Perucchini C, et al. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors. Cell. 2019;178:346-360.e24 pubmed publisher
Fachi J, Felipe J, Pral L, da Silva B, Corrêa R, de Andrade M, et al. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. Cell Rep. 2019;27:750-761.e7 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
Jung J, Yoo J, Choe Y, Park S, Lee H, Lee H, et al. Cleaved Cochlin Sequesters Pseudomonas aeruginosa and Activates Innate Immunity in the Inner Ear. Cell Host Microbe. 2019;25:513-525.e6 pubmed publisher
Silva H, Bafica A, Rodrigues Luiz G, Chi J, Santos P, Reis B, et al. Vasculature-associated fat macrophages readily adapt to inflammatory and metabolic challenges. J Exp Med. 2019;216:786-806 pubmed publisher
Blecher Gonen R, Bost P, Hilligan K, David E, Salame T, Roussel E, et al. Single-Cell Analysis of Diverse Pathogen Responses Defines a Molecular Roadmap for Generating Antigen-Specific Immunity. Cell Syst. 2019;8:109-121.e6 pubmed publisher
Britton G, Contijoch E, Mogno I, Vennaro O, Llewellyn S, Ng R, et al. Microbiotas from Humans with Inflammatory Bowel Disease Alter the Balance of Gut Th17 and RORγt+ Regulatory T Cells and Exacerbate Colitis in Mice. Immunity. 2019;50:212-224.e4 pubmed publisher
Willemze R, Welting O, van Hamersveld P, Verseijden C, Nijhuis L, Hilbers F, et al. Loss of intestinal sympathetic innervation elicits an innate immune driven colitis. Mol Med. 2019;25:1 pubmed publisher
Yao Y, Jeyanathan M, Haddadi S, Barra N, Vaseghi Shanjani M, Damjanovic D, et al. Induction of Autonomous Memory Alveolar Macrophages Requires T Cell Help and Is Critical to Trained Immunity. Cell. 2018;: pubmed publisher
Soberanes S, Misharin A, Jairaman A, Morales Nebreda L, McQuattie Pimentel A, Cho T, et al. Metformin Targets Mitochondrial Electron Transport to Reduce Air-Pollution-Induced Thrombosis. Cell Metab. 2019;29:335-347.e5 pubmed publisher
Jacobson A, Lam L, Rajendram M, Tamburini F, Honeycutt J, Pham T, et al. A Gut Commensal-Produced Metabolite Mediates Colonization Resistance to Salmonella Infection. Cell Host Microbe. 2018;24:296-307.e7 pubmed publisher
Hernández Santos N, Wiesner D, Fites J, McDermott A, Warner T, Wuthrich M, et al. Lung Epithelial Cells Coordinate Innate Lymphocytes and Immunity against Pulmonary Fungal Infection. Cell Host Microbe. 2018;23:511-522.e5 pubmed publisher
Pott J, Kabat A, Maloy K. Intestinal Epithelial Cell Autophagy Is Required to Protect against TNF-Induced Apoptosis during Chronic Colitis in Mice. Cell Host Microbe. 2018;23:191-202.e4 pubmed publisher
Danne C, Ryzhakov G, Martínez López M, Ilott N, Franchini F, Cuskin F, et al. A Large Polysaccharide Produced by Helicobacter hepaticus Induces an Anti-inflammatory Gene Signature in Macrophages. Cell Host Microbe. 2017;22:733-745.e5 pubmed publisher
Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
Yu X, Buttgereit A, Lelios I, Utz S, Cansever D, Becher B, et al. The Cytokine TGF-β Promotes the Development and Homeostasis of Alveolar Macrophages. Immunity. 2017;47:903-912.e4 pubmed publisher
Wang Y, Subramanian M, Yurdagul A, Barbosa Lorenzi V, Cai B, de Juan Sanz J, et al. Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages. Cell. 2017;171:331-345.e22 pubmed publisher
Varol D, Mildner A, Blank T, Shemer A, Barashi N, Yona S, et al. Dicer Deficiency Differentially Impacts Microglia of the Developing and Adult Brain. Immunity. 2017;46:1030-1044.e8 pubmed publisher
Ender F, Wiese A, Schmudde I, Sun J, Vollbrandt T, Konig P, et al. Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase. PLoS ONE. 2017;12:e0172446 pubmed publisher
Rao S, Schieber A, O Connor C, Leblanc M, Michel D, Ayres J. Pathogen-Mediated Inhibition of Anorexia Promotes Host Survival and Transmission. Cell. 2017;168:503-516.e12 pubmed publisher
Liu J, Xue Y, Dong D, Xiao C, Lin C, Wang H, et al. CCR2- and CCR2+ corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion. Mucosal Immunol. 2017;10:1145-1159 pubmed publisher
Sen D, Jones S, Oswald E, Pinkard H, Corbin K, Krummel M. Tracking the Spatial and Functional Gradient of Monocyte-To-Macrophage Differentiation in Inflamed Lung. PLoS ONE. 2016;11:e0165064 pubmed publisher
Hung W, Ling P, Cheng J, Chang S, Tseng C. Disabled-2 is a negative immune regulator of lipopolysaccharide-stimulated Toll-like receptor 4 internalization and signaling. Sci Rep. 2016;6:35343 pubmed publisher
Tan X, Fujiu K, Manabe I, Nishida J, Yamagishi R, Terashima Y, et al. Choroidal Neovascularization Is Inhibited in Splenic-Denervated or Splenectomized Mice with a Concomitant Decrease in Intraocular Macrophage. PLoS ONE. 2016;11:e0160985 pubmed publisher
Baptista M, Keszei M, Oliveira M, Sunahara K, Andersson J, Dahlberg C, et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016;7:12175 pubmed publisher
Denk F, Crow M, Didangelos A, Lopes D, McMahon S. Persistent Alterations in Microglial Enhancers in a Model of Chronic Pain. Cell Rep. 2016;15:1771-81 pubmed publisher
Collins N, Jiang X, Zaid A, Macleod B, Li J, Park C, et al. Skin CD4(+) memory T cells exhibit combined cluster-mediated retention and equilibration with the circulation. Nat Commun. 2016;7:11514 pubmed publisher
Terhorst D, Chelbi R, Wohn C, Malosse C, Tamoutounour S, Jorquera A, et al. Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis. J Immunol. 2015;195:4953-61 pubmed publisher
Evans H, Killoran K, Mitre B, Morris C, Kim S, Mitre E. Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice. J Immunol. 2015;195:2973-84 pubmed publisher
Cnops J, De Trez C, Stijlemans B, Keirsse J, Kauffmann F, Barkhuizen M, et al. NK-, NKT- and CD8-Derived IFNγ Drives Myeloid Cell Activation and Erythrophagocytosis, Resulting in Trypanosomosis-Associated Acute Anemia. PLoS Pathog. 2015;11:e1004964 pubmed publisher
Stijlemans B, Cnops J, Naniima P, Vaast A, Bockstal V, De Baetselier P, et al. Development of a pHrodo-based assay for the assessment of in vitro and in vivo erythrophagocytosis during experimental trypanosomosis. PLoS Negl Trop Dis. 2015;9:e0003561 pubmed publisher
Lee M, Shim D, Yoon J, Jang H, Oh S, Suh S, et al. Retnla overexpression attenuates allergic inflammation of the airway. PLoS ONE. 2014;9:e112666 pubmed publisher
van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, et al. Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells. J Immunol. 2015;194:307-15 pubmed publisher
Coquery C, Wade N, Loo W, Kinchen J, Cox K, Jiang C, et al. Neutrophils contribute to excess serum BAFF levels and promote CD4+ T cell and B cell responses in lupus-prone mice. PLoS ONE. 2014;9:e102284 pubmed publisher
Parsons M, Li L, Wallace A, Lee M, Katz H, Fernandez J, et al. Dectin-2 regulates the effector phase of house dust mite-elicited pulmonary inflammation independently from its role in sensitization. J Immunol. 2014;192:1361-71 pubmed publisher
Ozeri E, Mizrahi M, Shahaf G, Lewis E. ?-1 antitrypsin promotes semimature, IL-10-producing and readily migrating tolerogenic dendritic cells. J Immunol. 2012;189:146-53 pubmed publisher
Langlet C, Tamoutounour S, Henri S, Luche H, Ardouin L, Gregoire C, et al. CD64 expression distinguishes monocyte-derived and conventional dendritic cells and reveals their distinct role during intramuscular immunization. J Immunol. 2012;188:1751-60 pubmed publisher
Cho K, Suh J, Sohn J, Park J, Lee H, Kang J, et al. IL-33 induces Th17-mediated airway inflammation via mast cells in ovalbumin-challenged mice. Am J Physiol Lung Cell Mol Physiol. 2012;302:L429-40 pubmed publisher
Okamoto F, Saeki K, Sumimoto H, Yamasaki S, Yokomizo T. Leukotriene B4 augments and restores Fc gammaRs-dependent phagocytosis in macrophages. J Biol Chem. 2010;285:41113-21 pubmed publisher
product information
Antigen :
CD64
Apps. Abbrev. :
FC
Cat # :
139304
Clone :
X54-5/7.1
Item :
PE anti-mouse CD64 (FcγRI)
Isotype :
Mouse IgG1, κ
Other Names :
FcRI
Size :
100 μg
Price (USD) :
296 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Immunogen :
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Application Notes :
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation^1, and spatial biology (IBEX)^5,6. Clone X54-5/7.1 is not found to be useful for Western blots^1.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA