product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse CD335 (NKp46)
catalog :
137601
quantity :
50 μg
price :
101 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
29A1.4
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments, mass cytometry
more info or order :
citations: 73
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:50; loading ...; fig 6a
Drzyzga A, Cichon T, Czapla J, Jarosz Biej M, Pilny E, Matuszczak S, et al. The Proper Administration Sequence of Radiotherapy and Anti-Vascular Agent-DMXAA Is Essential to Inhibit the Growth of Melanoma Tumors. Cancers (Basel). 2021;13: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s1g
Tyagi A, Sharma S, Wu K, Wu S, Xing F, Liu Y, et al. Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre-metastatic niche in lung. Nat Commun. 2021;12:474 pubmed publisher
  • flow cytometry; mouse; loading ...
Lissner M, Cumnock K, Davis N, Vilches Moure J, Basak P, Navarrete D, et al. Metabolic profiling during malaria reveals the role of the aryl hydrocarbon receptor in regulating kidney injury. elife. 2020;9: pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1a, 1c, s1
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19c
Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Lecocq Q, Zeven K, De Vlaeminck Y, Martens S, Massa S, Goyvaerts C, et al. Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use. Biomolecules. 2019;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Martins J, Andoniou C, Fleming P, Kuns R, Schuster I, Voigt V, et al. Strain-specific antibody therapy prevents cytomegalovirus reactivation after transplantation. Science. 2019;363:288-293 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Vuckovic S, Minnie S, Smith D, Gartlan K, Watkins T, Markey K, et al. Bone marrow transplantation generates T cell-dependent control of myeloma in mice. J Clin Invest. 2019;129:106-121 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Almeida F, Tognarelli S, Marcais A, Kueh A, Friede M, Liao Y, et al. A point mutation in the Ncr1 signal peptide impairs the development of innate lymphoid cell subsets. Oncoimmunology. 2018;7:e1475875 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s8b
Pommier A, Anaparthy N, Memos N, Kelley Z, Gouronnec A, Yan R, et al. Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases. Science. 2018;360: pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Cribbs A, Hookway E, Wells G, Lindow M, Obad S, Oerum H, et al. Inhibition of histone H3K27 demethylases selectively modulates inflammatory phenotypes of natural killer cells. J Biol Chem. 2018;293:2422-2437 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7a
  • immunohistochemistry; mouse; loading ...; fig 2b
Kortlever R, Sodir N, Wilson C, Burkhart D, Pellegrinet L, Brown Swigart L, et al. Myc Cooperates with Ras by Programming Inflammation and Immune Suppression. Cell. 2017;171:1301-1315.e14 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s10a
Billerbeck E, Wolfisberg R, Fahnøe U, Xiao J, Quirk C, Luna J, et al. Mouse models of acute and chronic hepacivirus infection. Science. 2017;357:204-208 pubmed publisher
  • blocking or activating experiments; mouse; fig 3a
  • flow cytometry; mouse; loading ...
Shifrin N, Kissiov D, Ardolino M, Joncker N, Raulet D. Differential Role of Hematopoietic and Nonhematopoietic Cell Types in the Regulation of NK Cell Tolerance and Responsiveness. J Immunol. 2016;197:4127-4136 pubmed publisher
  • flow cytometry; mouse; fig s1
Kritikou J, Dahlberg C, Baptista M, Wagner A, Banerjee P, Gwalani L, et al. IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo. Sci Rep. 2016;6:30636 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 7a
Lawler C, Tan C, Simas J, Stevenson P. Type I Interferons and NK Cells Restrict Gammaherpesvirus Lymph Node Infection. J Virol. 2016;90:9046-57 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Qualai J, Li L, Cantero J, Tarrats A, Fernández M, Sumoy L, et al. Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential. PLoS ONE. 2016;11:e0154253 pubmed publisher
  • flow cytometry; mouse; 1:100; fig s4
Li Z, Hodgkinson T, Gothard E, Boroumand S, Lamb R, Cummins I, et al. Epidermal Notch1 recruits RORγ(+) group 3 innate lymphoid cells to orchestrate normal skin repair. Nat Commun. 2016;7:11394 pubmed publisher
  • flow cytometry; mouse; fig 1a
Gomez de Agüero M, Ganal Vonarburg S, Fuhrer T, Rupp S, Uchimura Y, Li H, et al. The maternal microbiota drives early postnatal innate immune development. Science. 2016;351:1296-302 pubmed publisher
  • flow cytometry; mouse; 1:20; loading ...; fig 2a
Osterloh A, Papp S, Moderzynski K, Kuehl S, Richardt U, Fleischer B. Persisting Rickettsia typhi Causes Fatal Central Nervous System Inflammation. Infect Immun. 2016;84:1615-1632 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Lin C, Bradstreet T, Schwarzkopf E, Jarjour N, Chou C, Archambault A, et al. IL-1-induced Bhlhe40 identifies pathogenic T helper cells in a model of autoimmune neuroinflammation. J Exp Med. 2016;213:251-71 pubmed publisher
  • flow cytometry; mouse; fig 5
Polansky J, Bahri R, Divivier M, Duitman E, Vock C, Goyeneche Patino D, et al. High dose CD11c-driven IL15 is sufficient to drive NK cell maturation and anti-tumor activity in a trans-presentation independent manner. Sci Rep. 2016;6:19699 pubmed publisher
  • flow cytometry; mouse; fig s5
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Lowe K, Navarro Núñez L, Bénézech C, Nayar S, Kingston B, Nieswandt B, et al. The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state. Eur J Immunol. 2015;45:2484-93 pubmed publisher
  • flow cytometry; mouse; fig s1
Kim M, Taparowsky E, Kim C. Retinoic Acid Differentially Regulates the Migration of Innate Lymphoid Cell Subsets to the Gut. Immunity. 2015;43:107-19 pubmed publisher
  • flow cytometry; mouse
Wickström S, Oberg L, Kärre K, Johansson M. A genetic defect in mice that impairs missing self recognition despite evidence for normal maturation and MHC class I-dependent education of NK cells. J Immunol. 2014;192:1577-86 pubmed publisher
Wan L, Lin K, Rahman M, Ishigami Y, Wang Z, Jensen M, et al. Splicing Factor SRSF1 Promotes Pancreatitis and KRASG12D-Mediated Pancreatic Cancer. Cancer Discov. 2023;13:1678-1695 pubmed publisher
Hu J, S xe1 nchez Rivera F, Wang Z, Johnson G, Ho Y, Ganesh K, et al. STING inhibits the reactivation of dormant metastasis in lung adenocarcinoma. Nature. 2023;616:806-813 pubmed publisher
Moore A, Vivanco Gonzalez N, Plummer K, Mitchel O, Kaur H, Rivera M, et al. Gestationally dependent immune organization at the maternal-fetal interface. Cell Rep. 2022;41:111651 pubmed publisher
Sodir N, Pellegrinet L, Kortlever R, Campos T, Kwon Y, Kim S, et al. Reversible Myc hypomorphism identifies a key Myc-dependency in early cancer evolution. Nat Commun. 2022;13:6782 pubmed publisher
Baldwin L, Bartonicek N, Yang J, Wu S, Deng N, Roden D, et al. DNA barcoding reveals ongoing immunoediting of clonal cancer populations during metastatic progression and immunotherapy response. Nat Commun. 2022;13:6539 pubmed publisher
Potempa M, Aguilar O, Gonzalez Hinojosa M, Tenvooren I, Marquez D, Spitzer M, et al. Influence of Self-MHC Class I Recognition on the Dynamics of NK Cell Responses to Cytomegalovirus Infection. J Immunol. 2022;208:1742-1754 pubmed publisher
Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, et al. Functional visualization of NK cell-mediated killing of metastatic single tumor cells. elife. 2022;11: pubmed publisher
Xiang H, Chan A, Ahene A, Bellovin D, Deng R, Hsu A, et al. Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer. MAbs. 2021;13:1981202 pubmed publisher
Goodall K, Nguyen A, Andrews D, Sullivan L. Ribosylation of the CD8αβ heterodimer permits binding of the nonclassical major histocompatibility molecule, H2-Q10. J Biol Chem. 2021;297:101141 pubmed publisher
Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
Wang Z, Goto Y, Allevato M, Wu V, Saddawi Konefka R, Gilardi M, et al. Disruption of the HER3-PI3K-mTOR oncogenic signaling axis and PD-1 blockade as a multimodal precision immunotherapy in head and neck cancer. Nat Commun. 2021;12:2383 pubmed publisher
Cribbs A, Filippakopoulos P, Philpott M, Wells G, Penn H, Oerum H, et al. Dissecting the Role of BET Bromodomain Proteins BRD2 and BRD4 in Human NK Cell Function. Front Immunol. 2021;12:626255 pubmed publisher
Can xe8 S, Van Snick J, Uyttenhove C, Pilotte L, van den Eynde B. TGFβ1 neutralization displays therapeutic efficacy through both an immunomodulatory and a non-immune tumor-intrinsic mechanism. J Immunother Cancer. 2021;9: pubmed publisher
Ma L, Gonzàlez Juncà A, Zheng Y, Ouyang H, Illa Bochaca I, Horst K, et al. Inflammation Mediates the Development of Aggressive Breast Cancer Following Radiotherapy. Clin Cancer Res. 2021;27:1778-1791 pubmed publisher
Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
Goh W, Scheer S, Jackson J, Hediyeh Zadeh S, Delconte R, Schuster I, et al. Hhex Directly Represses BIM-Dependent Apoptosis to Promote NK Cell Development and Maintenance. Cell Rep. 2020;33:108285 pubmed publisher
Bajic D, Chester K, Neri D. An Antibody-Tumor Necrosis Factor Fusion Protein that Synergizes with Oxaliplatin for Treatment of Colorectal Cancer. Mol Cancer Ther. 2020;19:2554-2563 pubmed publisher
Puca E, Schmitt Koopmann C, Furter M, Murer P, Probst P, Dihr M, et al. The targeted delivery of interleukin-12 to the carcinoembryonic antigen increases the intratumoral density of NK and CD8+ T cell in an immunocompetent mouse model of colorectal cancer. J Gastrointest Oncol. 2020;11:803-811 pubmed publisher
Wang C, Yan J, Yin P, Gui L, Ji L, Ma B, et al. β-Catenin inhibition shapes tumor immunity and synergizes with immunotherapy in colorectal cancer. Oncoimmunology. 2020;9:1809947 pubmed publisher
Aguilar O, Tanaka M, Balaji G, Berry R, Rossjohn J, Lanier L, et al. Tetramer Immunization and Selection Followed by CELLISA Screening to Generate Monoclonal Antibodies against the Mouse Cytomegalovirus m12 Immunoevasin. J Immunol. 2020;205:1709-1717 pubmed publisher
Martin E, Minet N, Boschat A, Sanquer S, Sobrino S, Lenoir C, et al. Impaired lymphocyte function and differentiation in CTPS1-deficient patients result from a hypomorphic homozygous mutation. JCI Insight. 2020;5: pubmed publisher
Yan J, Allen S, McDonald E, Das I, Mak J, Liu L, et al. MAIT Cells Promote Tumor Initiation, Growth, and Metastases via Tumor MR1. Cancer Discov. 2019;: pubmed publisher
Han H, Jain A, Truica M, Izquierdo Ferrer J, Anker J, Lysy B, et al. Small-Molecule MYC Inhibitors Suppress Tumor Growth and Enhance Immunotherapy. Cancer Cell. 2019;36:483-497.e15 pubmed publisher
Song C, Phuengkham H, Kim Y, Dinh V, Lee I, Shin I, et al. Syringeable immunotherapeutic nanogel reshapes tumor microenvironment and prevents tumor metastasis and recurrence. Nat Commun. 2019;10:3745 pubmed publisher
Westrich J, Vermeer D, Silva A, Bonney S, Berger J, Cicchini L, et al. CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8+ T-cell responses by upregulating MHC-I expression. Oncogene. 2019;: pubmed publisher
Petermann F, Pekowska A, Johnson C, Jankovic D, Shih H, Jiang K, et al. The Magnitude of IFN-γ Responses Is Fine-Tuned by DNA Architecture and the Non-coding Transcript of Ifng-as1. Mol Cell. 2019;75:1229-1242.e5 pubmed publisher
Puca E, Probst P, Stringhini M, Murer P, Pellegrini G, Cazzamalli S, et al. The antibody-based delivery of interleukin-12 to solid tumors boosts NK and CD8+ T cell activity and synergizes with immune checkpoint inhibitors. Int J Cancer. 2020;146:2518-2530 pubmed publisher
Ponzetta A, Carriero R, Carnevale S, Barbagallo M, Molgora M, Perucchini C, et al. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors. Cell. 2019;178:346-360.e24 pubmed publisher
Fausther Bovendo H, Qiu X, He S, Bello A, Audet J, Ippolito G, et al. NK Cells Accumulate in Infected Tissues and Contribute to Pathogenicity of Ebola Virus in Mice. J Virol. 2019;93: pubmed publisher
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed publisher
Du X, de Almeida P, Manieri N, de Almeida Nagata D, Wu T, Harden Bowles K, et al. CD226 regulates natural killer cell antitumor responses via phosphorylation-mediated inactivation of transcription factor FOXO1. Proc Natl Acad Sci U S A. 2018;115:E11731-E11740 pubmed publisher
Crinier A, Milpied P, Escalière B, Piperoglou C, Galluso J, Balsamo A, et al. High-Dimensional Single-Cell Analysis Identifies Organ-Specific Signatures and Conserved NK Cell Subsets in Humans and Mice. Immunity. 2018;49:971-986.e5 pubmed publisher
Gubin M, Esaulova E, Ward J, Malkova O, Runci D, Wong P, et al. High-Dimensional Analysis Delineates Myeloid and Lymphoid Compartment Remodeling during Successful Immune-Checkpoint Cancer Therapy. Cell. 2018;175:1014-1030.e19 pubmed publisher
Ito Y, Ashenberg O, Pyrdol J, Luoma A, Rozenblatt Rosen O, Hofree M, et al. Rapid CLIP dissociation from MHC II promotes an unusual antigen presentation pathway in autoimmunity. J Exp Med. 2018;215:2617-2635 pubmed publisher
Hansell C, Fraser A, Hayes A, Pingen M, Burt C, Lee K, et al. The Atypical Chemokine Receptor Ackr2 Constrains NK Cell Migratory Activity and Promotes Metastasis. J Immunol. 2018;201:2510-2519 pubmed publisher
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
Thompson T, Jackson B, Li P, Wang J, Kim A, Huang K, et al. Tumor-derived CSF-1 induces the NKG2D ligand RAE-1δ on tumor-infiltrating macrophages. elife. 2018;7: pubmed publisher
Ramani K, Jawale C, Verma A, Coleman B, Kolls J, Biswas P. Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection. JCI Insight. 2018;3: pubmed publisher
Treise I, Huber E, Klein Rodewald T, Heinemeyer W, Grassmann S, Basler M, et al. Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. Sci Rep. 2018;8:5975 pubmed publisher
Lázaro Frías A, Gomez Medina S, Sanchez Sampedro L, Ljungberg K, Ustav M, Liljestrom P, et al. Distinct Immunogenicity and Efficacy of Poxvirus-Based Vaccine Candidates against Ebola Virus Expressing GP and VP40 Proteins. J Virol. 2018;92: pubmed publisher
Staaf E, Hedde P, Bagawath Singh S, Piguet J, Gratton E, Johansson S. Educated natural killer cells show dynamic movement of the activating receptor NKp46 and confinement of the inhibitory receptor Ly49A. Sci Signal. 2018;11: pubmed publisher
Glasner A, Isaacson B, Viukov S, Neuman T, Friedman N, Mandelboim M, et al. Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression. Sci Rep. 2017;7:13090 pubmed publisher
Nussbaum K, Burkhard S, Ohs I, Mair F, Klose C, Arnold S, et al. Tissue microenvironment dictates the fate and tumor-suppressive function of type 3 ILCs. J Exp Med. 2017;214:2331-2347 pubmed publisher
Wagner A, Kadri N, Snäll J, Brodin P, Gilfillan S, Colonna M, et al. Expression of CD226 is associated to but not required for NK cell education. Nat Commun. 2017;8:15627 pubmed publisher
Di Pilato M, Mejías Pérez E, Sorzano C, Esteban M. Distinct Roles of Vaccinia Virus NF-κB Inhibitor Proteins A52, B15, and K7 in the Immune Response. J Virol. 2017;91: pubmed publisher
Guerriero J, Ditsworth D, Catanzaro J, Sabino G, Furie M, Kew R, et al. DNA alkylating therapy induces tumor regression through an HMGB1-mediated activation of innate immunity. J Immunol. 2011;186:3517-26 pubmed publisher
product information
Antigen :
CD335
Apps. Abbrev. :
FC, IHC-F
Cat # :
137601
Clone :
29A1.4
Item :
Purified anti-mouse CD335 (NKp46)
Isotype :
Rat IgG2a, κ
Other Names :
NKp46, NCR1
Size :
50 μg
Price (USD) :
101 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
NKP46-IgG1 Fc fusion protein
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1,2 and in vitro activation of NK cells^1.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA