product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse/human Helios
catalog :
137218
quantity :
100 tests
price :
405 USD
clonality :
monoclonal
host :
hamsters
conjugate :
AF647
clone name :
22F6
reactivity :
human, mouse, rhesus macaque
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:50; loading ...; fig 3i
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig 1b
Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Mittelstadt P, Taves M, Ashwell J. Cutting Edge: De Novo Glucocorticoid Synthesis by Thymic Epithelial Cells Regulates Antigen-Specific Thymocyte Selection. J Immunol. 2018;200:1988-1994 pubmed publisher
  • flow cytometry; rhesus macaque; fig 3c
Rueda C, Presicce P, Jackson C, Miller L, Kallapur S, Jobe A, et al. Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque. J Immunol. 2016;196:3706-15 pubmed publisher
  • flow cytometry; human
Käser T, Mair K, Hammer S, Gerner W, Saalmüller A. Natural and inducible Tregs in swine: Helios expression and functional properties. Dev Comp Immunol. 2015;49:323-31 pubmed publisher
Cossarizza A, Chang H, Radbruch A, Abrignani S, Addo R, Akdis M, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). Eur J Immunol. 2021;51:2708-3145 pubmed publisher
Hu W, Wang Z, Feng Y, Schizas M, Hoyos B, van der Veeken J, et al. Regulatory T cells function in established systemic inflammation and reverse fatal autoimmunity. Nat Immunol. 2021;22:1163-1174 pubmed publisher
Lam A, Lin D, Gillies J, Uday P, Pesenacker A, Kobor M, et al. Optimized CRISPR-mediated gene knockin reveals FOXP3-independent maintenance of human Treg identity. Cell Rep. 2021;36:109494 pubmed publisher
Kawakami R, Kitagawa Y, Chen K, Arai M, Ohara D, Nakamura Y, et al. Distinct Foxp3 enhancer elements coordinate development, maintenance, and function of regulatory T cells. Immunity. 2021;54:947-961.e8 pubmed publisher
Russler Germain E, Yi J, Young S, Nutsch K, Wong H, Ai T, et al. Gut Helicobacter presentation by multiple dendritic cell subsets enables context-specific regulatory T cell generation. elife. 2021;10: pubmed publisher
Chopp L, Gopalan V, Ciucci T, Ruchinskas A, Rae Z, Lagarde M, et al. An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development. Immunity. 2020;53:1182-1201.e8 pubmed publisher
Benner M, Feyaerts D, García C, Inci N, López S, Fasse E, et al. Clusters of Tolerogenic B Cells Feature in the Dynamic Immunological Landscape of the Pregnant Uterus. Cell Rep. 2020;32:108204 pubmed publisher
Goodwin M, Lee E, Lakshmanan U, Shipp S, Froessl L, Barzaghi F, et al. CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells. Sci Adv. 2020;6:eaaz0571 pubmed publisher
Dean J, Peters L, Fuhrman C, Seay H, Posgai A, Stimpson S, et al. Innate inflammation drives NK cell activation to impair Treg activity. J Autoimmun. 2020;108:102417 pubmed publisher
Sidwell T, Liao Y, Garnham A, Vasanthakumar A, Gloury R, Blume J, et al. Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis. Nat Commun. 2020;11:252 pubmed publisher
Becher J, Simula L, Volpe E, Procaccini C, La Rocca C, D Acunzo P, et al. AMBRA1 Controls Regulatory T-Cell Differentiation and Homeostasis Upstream of the FOXO3-FOXP3 Axis. Dev Cell. 2018;47:592-607.e6 pubmed publisher
Kim Y, Kim K, Yoon J, Scott D, Shevach E. SAMHD1 Posttranscriptionally Controls the Expression of Foxp3 and Helios in Human T Regulatory Cells. J Immunol. 2018;201:1671-1680 pubmed publisher
Landman S, Cruijsen M, Urbano P, Huls G, van Erp P, van Rijssen E, et al. DNA Methyltransferase Inhibition Promotes Th1 Polarization in Human CD4+CD25high FOXP3+ Regulatory T Cells but Does Not Affect Their Suppressive Capacity. J Immunol Res. 2018;2018:4973964 pubmed publisher
Allenspach E, Finn L, Rendi M, Eken A, Singh A, Oukka M, et al. Absence of functional fetal regulatory T cells in humans causes in utero organ-specific autoimmunity. J Allergy Clin Immunol. 2017;140:616-619.e7 pubmed publisher
He X, Smeets R, van Rijssen E, Boots A, Joosten I, Koenen H. Single CD28 stimulation induces stable and polyclonal expansion of human regulatory T cells. Sci Rep. 2017;7:43003 pubmed publisher
Fuhrman C, Yeh W, Seay H, Saikumar Lakshmi P, Chopra G, Zhang L, et al. Divergent Phenotypes of Human Regulatory T Cells Expressing the Receptors TIGIT and CD226. J Immunol. 2015;195:145-55 pubmed publisher
Verhagen J, Wraith D. Blockade of LFA-1 augments in vitro differentiation of antigen-induced Foxp3? Treg cells. J Immunol Methods. 2014;414:58-64 pubmed publisher
Liu Y, Tran D, Fatheree N, Marc Rhoads J. Lactobacillus reuteri DSM 17938 differentially modulates effector memory T cells and Foxp3+ regulatory T cells in a mouse model of necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol. 2014;307:G177-86 pubmed publisher
Bedke T, Iannitti R, De Luca A, Giovannini G, Fallarino F, Berges C, et al. Distinct and complementary roles for Aspergillus fumigatus-specific Tr1 and Foxp3+ regulatory T cells in humans and mice. Immunol Cell Biol. 2014;92:659-70 pubmed publisher
Angin M, Klarenbeek P, King M, Sharma S, Moodley E, Rezai A, et al. Regulatory T cells expanded from HIV-1-infected individuals maintain phenotype, TCR repertoire and suppressive capacity. PLoS ONE. 2014;9:e86920 pubmed publisher
Föhse L, Reinhardt A, Oberdörfer L, Schmitz S, Forster R, Malissen B, et al. Differential postselection proliferation dynamics of ?? T cells, Foxp3+ regulatory T cells, and invariant NKT cells monitored by genetic pulse labeling. J Immunol. 2013;191:2384-92 pubmed publisher
Paiva R, Lino A, Bergman M, Caramalho I, Sousa A, Zelenay S, et al. Recent thymic emigrants are the preferential precursors of regulatory T cells differentiated in the periphery. Proc Natl Acad Sci U S A. 2013;110:6494-9 pubmed publisher
Schoenbrunn A, Frentsch M, Kohler S, Keye J, Dooms H, Moewes B, et al. A converse 4-1BB and CD40 ligand expression pattern delineates activated regulatory T cells (Treg) and conventional T cells enabling direct isolation of alloantigen-reactive natural Foxp3+ Treg. J Immunol. 2012;189:5985-94 pubmed publisher
Stone B, Rieck M, Rawlings C, Kas A, Shendure J, Jones H, et al. Identification of novel HLA class II target epitopes for generation of donor-specific T regulatory cells. Clin Immunol. 2012;145:153-60 pubmed publisher
Vaeth M, Schliesser U, Müller G, Reissig S, Satoh K, Tuettenberg A, et al. Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A. 2012;109:16258-63 pubmed publisher
Wainwright D, Sengupta S, Han Y, Lesniak M. Thymus-derived rather than tumor-induced regulatory T cells predominate in brain tumors. Neuro Oncol. 2011;13:1308-23 pubmed publisher
Thornton A, Korty P, Tran D, Wohlfert E, Murray P, Belkaid Y, et al. Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells. J Immunol. 2010;184:3433-41 pubmed publisher
product information
Antigen :
Helios
Apps. Abbrev. :
ICFC
Cat # :
137218
Clone :
22F6
Item :
Alexa Fluor® 647 anti-mouse/human Helios
Isotype :
Armenian Hamster IgG
Other Names :
IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Size :
100 tests
Price (USD) :
405 USD
Reactivity :
Mouse, Human
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
A647
Immunogen :
Helios peptide (aa 51-107)
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA