One Step Staining Mouse Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD25 PE/CD4 PerCP)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

One Step Staining Mouse Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD25 PE/CD4 PerCP)

catalog :

136801

quantity :

25 tests

price :

500 USD

clonality :

monoclonal

host :

rat

conjugate :

AF488

reactivity :

mouse

application :

flow cytometry

citations: 9

Published Application/Species/Dilution	Reference
flow cytometry; mouse	Boyoglu-Barnum S, Chirkova T, Todd S, Barnum T, Gaston K, Jorquera P, et al. Prophylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice. J Virol. 2014;88:10569-83 pubmed publisher
	Gao L, Liu F, Tan L, Liu T, Chen Z, Shi C. The immunosuppressive properties of non-cultured dermal-derived mesenchymal stromal cells and the control of graft-versus-host disease. Biomaterials. 2014;35:3582-8 pubmed publisher
	Bhattacharya D, Dwivedi V, Kumar S, Reddy M, Van Kaer L, Moodley P, et al. Simultaneous inhibition of T helper 2 and T regulatory cell differentiation by small molecules enhances Bacillus Calmette-Guerin vaccine efficacy against tuberculosis. J Biol Chem. 2014;289:33404-11 pubmed publisher
	Amoah S, Holbrook B, Yammani R, Alexander-Miller M. High viral burden restricts short-lived effector cell number at late times postinfection through increased natural regulatory T cell expansion. J Immunol. 2013;190:5020-9 pubmed publisher
	Nakashima H, Hamaguchi Y, Watanabe R, Ishiura N, Kuwano Y, Okochi H, et al. CD22 expression mediates the regulatory functions of peritoneal B-1a cells during the remission phase of contact hypersensitivity reactions. J Immunol. 2010;184:4637-45 pubmed publisher
	Horikoshi M, Goto D, Segawa S, Yoshiga Y, Iwanami K, Inoue A, et al. Activation of Invariant NKT cells with glycolipid ligand α-galactosylceramide ameliorates glucose-6-phosphate isomerase peptide-induced arthritis. PLoS ONE. 2012;7:e51215 pubmed publisher
	Sugiura D, Denda-Nagai K, Takashima M, Murakami R, Nagai S, Takeda K, et al. Local effects of regulatory T cells in MUC1 transgenic mice potentiate growth of MUC1 expressing tumor cells in vivo. PLoS ONE. 2012;7:e44770 pubmed publisher
	Barber A, Rynda A, Sentman C. Chimeric NKG2D expressing T cells eliminate immunosuppression and activate immunity within the ovarian tumor microenvironment. J Immunol. 2009;183:6939-47 pubmed publisher
	Fallarino F, Volpi C, Zelante T, Vacca C, Calvitti M, Fioretti M, et al. IDO mediates TLR9-driven protection from experimental autoimmune diabetes. J Immunol. 2009;183:6303-12 pubmed publisher

product information

Antigen :

FOXP3/CD4/CD25

Apps. Abbrev. :

Flow Cytometry, Intracellular Staining for Flow Cytometry

Cat # :

136801

Clone :

MF-14

Item :

One Step Staining Mouse Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD25 PE/CD4 PerCP)

Isotype :

Rat IgG2b, κ

Other Names :

Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2

Size :

25 tests

Price (USD) :

500 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Application Notes :

Materials Provided: 1. Alexa Fluor 488 anti-mouse FOXP3/CD25 PE/CD4 PerCP antibody cocktail, 25 tests 2. Alexa Fluor 488 Rat IgG2b, isotype control/CD25 PE/CD4 PerCP antibody cocktail, 25 tests 3. FOXP3 Fix/Perm Buffer Set, 100 tests Cat. No. 421403 Materials not included: Cell Staining Buffer (Cat. No. 420201) Single color compensation controls Immunofluorescence Staining Procedures: Centrifugation steps: perform at 250Xg for 5min Incubation steps: perform at room temperature. 1. Prepare 1X working solutions of FOXP3 Fix/Perm buffer and FOXP3 Perm buffer prior to staining. The FOXP3 Fix/Perm buffer (4X) must be freshly diluted by diluting one (1) part FOPX3 Fix/Perm buffer (4X) with three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted by diluting one (1) part FOXP3 Perm buffer (10X) with nine (9) parts PBS. NOTE : The FOXP3 Perm buffer (10X) may have crystalization or precipitation observed when it is stored at 2-8 C, however, it is normal and does not affect the buffer performance. If there is heavy precipitation observed after diluted to 1X working solution, it can be filtered to clarify the solution. Caution : The FOXP3 Fix/Perm buffer contains paraformaldehyde, which is toxic and mutagenic. Please wear necessary protection to avoid direct body contact. 2. Suspend the cells of interest at 1X 10 7 /ml in BioLegend's cell staining buffer (Cat. No. 420201). 3. Distribute 100 ul of the cell suspension into each FACS tube. 4. Add 1 ml of 1X BioLegend's FOXP3 Fix/Perm solution to each tube, vortex and incubate for 20 minutes; centrifuge, then discard the supernatant. 5. Wash: resuspend cells in 2ml/tube cell staining buffer; centrifuge, then discard the supernatant. 6. Wash: resuspend in 1ml 1X BioLegend's FOXP3 Perm buffer; centrifuge, then discard the supernatent. 7. Resuspend cells in 1ml 1X BioLegend's FOXP3 Perm buffer, incubate in the dark for 15 minutes; centrifuge, then discard the supernatant. 8. Add 20 ul Alexa Fluor 488 anti-mouse FOXP3/CD25 PE/CD4 PerCP antibody cocktail or 20 ul Alexa Fluor 488 rat IgG2b, isotype control/CD25 PE/CD4 PerCP antibody cocktail into appropriate tube, and resuspend the cell pellet by gentle vortex. Incubate in the dark for 30 minutes. 9. Wash twice with cell staining buffer (see step 5) and resuspend in 0.5ml cell staining buffer then analyze with flow cytometer using appropriate instrument settings.

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