product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 594 anti-mouse CD115 (CSF-1R)
catalog :
135520
quantity :
100 μg
price :
288 USD
clonality :
monoclonal
host :
rat
conjugate :
Alexa Fluor 594
clone name :
AFS98
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, mass cytometry
more info or order :
citations: 43
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s3a
Fahy N, Palomares Cabeza V, Lolli A, Witte Bouma J, Merino A, Ridwan Y, et al. Chondrogenically Primed Human Mesenchymal Stem Cells Persist and Undergo Early Stages of Endochondral Ossification in an Immunocompetent Xenogeneic Model. Front Immunol. 2021;12:715267 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Gurley J, Gmyrek G, Hargis E, Bishop G, Carr D, Elliott M. The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation. Cells. 2021;10: pubmed publisher
  • flow cytometry; mouse; fig 4c
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6f
Wang S, Li R, Ng T, Luthria G, Oudin M, Prytyskach M, et al. Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy. Sci Adv. 2020;6:eaaz8521 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 3s3c
Burfeind K, Zhu X, Norgard M, Levasseur P, Huisman C, Buenafe A, et al. Circulating myeloid cells invade the central nervous system to mediate cachexia during pancreatic cancer. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 1a
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; fig s7
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • mass cytometry; mouse; 0.75 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1a, 1c, s1
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig ex3a
Croft A, Campos J, Jansen K, Turner J, Marshall J, Attar M, et al. Distinct fibroblast subsets drive inflammation and damage in arthritis. Nature. 2019;570:246-251 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig e7b
Jacome Galarza C, Percin G, Muller J, Mass E, Lazarov T, Eitler J, et al. Developmental origin, functional maintenance and genetic rescue of osteoclasts. Nature. 2019;568:541-545 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Lund H, Pieber M, Parsa R, Han J, Grommisch D, Ewing E, et al. Competitive repopulation of an empty microglial niche yields functionally distinct subsets of microglia-like cells. Nat Commun. 2018;9:4845 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s9b
Choi H, Suwanpradid J, Kim I, Staats H, Haniffa M, Macleod A, et al. Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. Science. 2018;362: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 1c
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Capucha T, Koren N, Nassar M, Heyman O, Nir T, Levy M, et al. Sequential BMP7/TGF-β1 signaling and microbiota instruct mucosal Langerhans cell differentiation. J Exp Med. 2018;215:481-500 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; fig 1a
Anderson D, Grajales Reyes G, Satpathy A, Vasquez Hueichucura C, Murphy T, Murphy K. Revisiting the specificity of the MHC class?II transactivator CIITA in classical murine dendritic cells in vivo. Eur J Immunol. 2017;47:1317-1323 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5
Sharif S, Nakatani Y, Wise L, Corbett M, Real N, Stuart G, et al. A Broad-Spectrum Chemokine-Binding Protein of Bovine Papular Stomatitis Virus Inhibits Neutrophil and Monocyte Infiltration in Inflammatory and Wound Models of Mouse Skin. PLoS ONE. 2016;11:e0168007 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
Kastelberg B, Tubau Juni N, Ayubi T, Leung A, Leber A, Hontecillas R, et al. NLRX1 is a key regulator of immune signaling during invasive pulmonary aspergillosis. PLoS Pathog. 2020;16:e1008854 pubmed publisher
Pijuan Sala B, Griffiths J, Guibentif C, Hiscock T, Jawaid W, Calero Nieto F, et al. A single-cell molecular map of mouse gastrulation and early organogenesis. Nature. 2019;566:490-495 pubmed publisher
Kuboi Y, Nishimura M, Ikeda W, Nakatani T, Seki Y, Yamaura Y, et al. Blockade of the fractalkine-CX3CR1 axis ameliorates experimental colitis by dislodging venous crawling monocytes. Int Immunol. 2019;31:287-302 pubmed publisher
Otxoa de Amezaga A, Miro Mur F, Pedragosa J, Gallizioli M, Justicia C, Gaja Capdevila N, et al. Microglial cell loss after ischemic stroke favors brain neutrophil accumulation. Acta Neuropathol. 2019;137:321-341 pubmed publisher
Mouhadeb O, Ben Shlomo S, Cohen K, Farkash I, Gruber S, Maharshak N, et al. Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function. Front Immunol. 2018;9:2623 pubmed publisher
Miyachi Y, Tsuchiya K, Shiba K, Mori K, Komiya C, Ogasawara N, et al. A reduced M1-like/M2-like ratio of macrophages in healthy adipose tissue expansion during SGLT2 inhibition. Sci Rep. 2018;8:16113 pubmed publisher
Cabron A, El Azzouzi K, Boss M, Arnold P, Schwarz J, Rosas M, et al. Structural and Functional Analyses of the Shedding Protease ADAM17 in HoxB8-Immortalized Macrophages and Dendritic-like Cells. J Immunol. 2018;201:3106-3118 pubmed publisher
Youssif C, Cubillos Rojas M, Comalada M, Llonch E, Perna C, Djouder N, et al. Myeloid p38α signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis. EMBO Mol Med. 2018;10: pubmed publisher
Shaw T, Houston S, Wemyss K, Bridgeman H, Barbera T, Zangerle Murray T, et al. Tissue-resident macrophages in the intestine are long lived and defined by Tim-4 and CD4 expression. J Exp Med. 2018;215:1507-1518 pubmed publisher
Youssef L, Rebbaa A, Pampou S, Weisberg S, Stockwell B, Hod E, et al. Increased erythrophagocytosis induces ferroptosis in red pulp macrophages in a mouse model of transfusion. Blood. 2018;: pubmed publisher
Costa M, Kudaravalli J, Liu W, Stock J, Kong S, Liu S. A mouse model for evaluation of efficacy and concomitant toxicity of anti-human CXCR4 therapeutics. PLoS ONE. 2018;13:e0194688 pubmed publisher
Huang L, Nazarova E, Tan S, Liu Y, Russell D. Growth of Mycobacterium tuberculosis in vivo segregates with host macrophage metabolism and ontogeny. J Exp Med. 2018;215:1135-1152 pubmed publisher
Jang J, Li J, Gambini L, Batugedara H, Sati S, Lazar M, et al. Human resistin protects against endotoxic shock by blocking LPS-TLR4 interaction. Proc Natl Acad Sci U S A. 2017;114:E10399-E10408 pubmed publisher
Poulos M, Ramalingam P, Gutkin M, Llanos P, Gilleran K, Rabbany S, et al. Endothelial transplantation rejuvenates aged hematopoietic stem cell function. J Clin Invest. 2017;127:4163-4178 pubmed publisher
Mossadegh Keller N, Gentek R, Gimenez G, Bigot S, Mailfert S, Sieweke M. Developmental origin and maintenance of distinct testicular macrophage populations. J Exp Med. 2017;214:2829-2841 pubmed publisher
Llewellyn R, Thomas K, Gutknecht M, Bouton A. The nonreceptor protein tyrosine kinase Pyk2 promotes the turnover of monocytes at steady state. J Leukoc Biol. 2017;102:1069-1080 pubmed publisher
Parashar K, Kopping E, Frank D, Sampath V, Thanassi D, Carpino N. Increased Resistance to Intradermal Francisella tularensis LVS Infection by Inactivation of the Sts Phosphatases. Infect Immun. 2017;85: pubmed publisher
Wolf Y, Shemer A, Polonsky M, Gross M, Mildner A, Yona S, et al. Autonomous TNF is critical for in vivo monocyte survival in steady state and inflammation. J Exp Med. 2017;214:905-917 pubmed publisher
Cuccarese M, Dubach J, Pfirschke C, Engblom C, Garris C, Miller M, et al. Heterogeneity of macrophage infiltration and therapeutic response in lung carcinoma revealed by 3D organ imaging. Nat Commun. 2017;8:14293 pubmed publisher
Kretzer N, Theisen D, Tussiwand R, Briseño C, Grajales Reyes G, Wu X, et al. RAB43 facilitates cross-presentation of cell-associated antigens by CD8?+ dendritic cells. J Exp Med. 2016;213:2871-2883 pubmed
Li M, Bozzacco L, Hoffmann H, Breton G, Loschko J, Xiao J, et al. Interferon regulatory factor 2 protects mice from lethal viral neuroinvasion. J Exp Med. 2016;213:2931-2947 pubmed
Meierovics A, Cowley S. MAIT cells promote inflammatory monocyte differentiation into dendritic cells during pulmonary intracellular infection. J Exp Med. 2016;213:2793-2809 pubmed
Afik R, Zigmond E, Vugman M, Klepfish M, Shimshoni E, Pasmanik Chor M, et al. Tumor macrophages are pivotal constructors of tumor collagenous matrix. J Exp Med. 2016;213:2315-2331 pubmed
Jaeger B, Donadieu J, Cognet C, Bernat C, Ordoñez Rueda D, Barlogis V, et al. Neutrophil depletion impairs natural killer cell maturation, function, and homeostasis. J Exp Med. 2012;209:565-80 pubmed publisher
product information
Antigen :
CD115
Apps. Abbrev. :
ICC
Cat # :
135520
Clone :
AFS98
Item :
Alexa Fluor® 594 anti-mouse CD115 (CSF-1R)
Isotype :
Rat IgG2a, κ
Other Names :
CSF-1R, M-CSFR, c-fms
Size :
100 μg
Price (USD) :
288 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A594
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of ligand binding 1 . The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD115 4 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA