product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD115 (CSF-1R)
catalog :
135505
quantity :
50 μg
price :
117 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
AFS98
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 53
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s3a
Fahy N, Palomares Cabeza V, Lolli A, Witte Bouma J, Merino A, Ridwan Y, et al. Chondrogenically Primed Human Mesenchymal Stem Cells Persist and Undergo Early Stages of Endochondral Ossification in an Immunocompetent Xenogeneic Model. Front Immunol. 2021;12:715267 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Voisin M, Shrestha E, Rollet C, Nikain C, Josefs T, Mahe M, et al. Inhibiting LXRα phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice. Commun Biol. 2021;4:420 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 1a
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 3c
Garcia Agudo L, Janova H, Sendler L, Arinrad S, Steixner A, Hassouna I, et al. Genetically induced brain inflammation by Cnp deletion transiently benefits from microglia depletion. FASEB J. 2019;33:8634-8647 pubmed publisher
  • flow cytometry; mouse; 1:300; fig 5
Aryal B, Rotllan N, Araldi E, Ramírez C, He S, Chousterman B, et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016;7:12313 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
  • flow cytometry; mouse; fig 3a
Stoilova B, Kowenz Leutz E, Scheller M, Leutz A. Lymphoid to myeloid cell trans-differentiation is determined by C/EBP? structure and post-translational modifications. PLoS ONE. 2013;8:e65169 pubmed publisher
Johnson K, Soukup A, Bresnick E. GATA2 deficiency elevates interferon regulatory factor-8 to subvert a progenitor cell differentiation program. Blood Adv. 2022;6:1464-1473 pubmed publisher
Groenen A, La Rose A, Li M, Bazioti V, Svendsen A, Kloosterhuis N, et al. Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 disease. J Lipid Res. 2022;63:100167 pubmed publisher
Kuhn J, Vainchtein I, Braz J, Hamel K, Bernstein M, Craik V, et al. Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice. elife. 2021;10: pubmed publisher
Jütte B, Krollmann C, Cieslak K, Koerber R, Boor P, Graef C, et al. Intercellular cGAMP transmission induces innate immune activation and tissue inflammation in Trex1 deficiency. iScience. 2021;24:102833 pubmed publisher
Wang W, Li R, Miao W, Evans C, Lu L, Lyu J, et al. Development and Evaluation of a Novel Mouse Model of Asphyxial Cardiac Arrest Revealed Severely Impaired Lymphopoiesis After Resuscitation. J Am Heart Assoc. 2021;10:e019142 pubmed publisher
LeBlond N, Ghorbani P, O Dwyer C, Ambursley N, Nunes J, Smith T, et al. Myeloid deletion and therapeutic activation of AMPK do not alter atherosclerosis in male or female mice. J Lipid Res. 2020;61:1697-1706 pubmed publisher
Fuster J, Zuriaga M, Zorita V, MacLauchlan S, Polackal M, Viana Huete V, et al. TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity. Cell Rep. 2020;33:108326 pubmed publisher
Tripathi H, Al Darraji A, Abo Aly M, Peng H, Shokri E, Chelvarajan L, et al. Autotaxin inhibition reduces cardiac inflammation and mitigates adverse cardiac remodeling after myocardial infarction. J Mol Cell Cardiol. 2020;149:95-114 pubmed publisher
Hreha T, Collins C, Daugherty A, Griffith J, Hruska K, Hunstad D. Androgen-Influenced Polarization of Activin A-Producing Macrophages Accompanies Post-pyelonephritic Renal Scarring. Front Immunol. 2020;11:1641 pubmed publisher
Bouchareychas L, Duong P, Covarrubias S, Alsop E, Phu T, Chung A, et al. Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo. Cell Rep. 2020;32:107881 pubmed publisher
Tran S, Baba I, Poupel L, Dussaud S, Moreau M, Gélineau A, et al. Impaired Kupffer Cell Self-Renewal Alters the Liver Response to Lipid Overload during Non-alcoholic Steatohepatitis. Immunity. 2020;53:627-640.e5 pubmed publisher
Tripathi H, Peng H, Donahue R, Chelvarajan L, Gottipati A, Levitan B, et al. Isolation Methods for Human CD34 Subsets Using Fluorescent and Magnetic Activated Cell Sorting: an In Vivo Comparative Study. Stem Cell Rev Rep. 2020;16:413-423 pubmed publisher
Jain A, Irizarry Caro R, McDaniel M, Chawla A, Carroll K, Overcast G, et al. T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunity. Nat Immunol. 2020;21:65-74 pubmed publisher
Sakai M, Troutman T, Seidman J, Ouyang Z, Spann N, Abe Y, et al. Liver-Derived Signals Sequentially Reprogram Myeloid Enhancers to Initiate and Maintain Kupffer Cell Identity. Immunity. 2019;51:655-670.e8 pubmed publisher
Burgener S, Leborgne N, Snipas S, Salvesen G, Bird P, Benarafa C. Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven Inflammation. Cell Rep. 2019;27:3646-3656.e5 pubmed publisher
Anderson S, Roberts J, Zhang J, Steele M, Romero C, Bosco A, et al. Developmental Apoptosis Promotes a Disease-Related Gene Signature and Independence from CSF1R Signaling in Retinal Microglia. Cell Rep. 2019;27:2002-2013.e5 pubmed publisher
Barman P, Pang J, Urao N, Koh T. Skin Wounding-Induced Monocyte Expansion in Mice Is Not Abrogated by IL-1 Receptor 1 Deficiency. J Immunol. 2019;202:2720-2727 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Di Liberto G, Pantelyushin S, Kreutzfeldt M, Page N, Musardo S, Coras R, et al. Neurons under T Cell Attack Coordinate Phagocyte-Mediated Synaptic Stripping. Cell. 2018;175:458-471.e19 pubmed publisher
Combes F, Mc Cafferty S, Meyer E, Sanders N. Off-Target and Tumor-Specific Accumulation of Monocytes, Macrophages and Myeloid-Derived Suppressor Cells after Systemic Injection. Neoplasia. 2018;20:848-856 pubmed publisher
Lee M, Chakhtoura M, Sriram U, Caricchio R, Gallucci S. Conventional DCs from Male and Female Lupus-Prone B6.NZM Sle1/Sle2/Sle3 Mice Express an IFN Signature and Have a Higher Immunometabolism That Are Enhanced by Estrogen. J Immunol Res. 2018;2018:1601079 pubmed publisher
Clemente C, Rius C, Alonso Herranz L, Martín Alonso M, Pollán A, Camafeita E, et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun. 2018;9:910 pubmed publisher
Evrard M, Kwok I, Chong S, Teng K, Becht E, Chen J, et al. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity. 2018;48:364-379.e8 pubmed publisher
Petriello M, Brandon J, Hoffman J, Wang C, Tripathi H, Abdel Latif A, et al. Dioxin-like PCB 126 increases systemic inflammation and accelerates atherosclerosis in lean LDL receptor deficient mice. Toxicol Sci. 2017;: pubmed publisher
Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
Yáñez A, Coetzee S, Olsson A, Muench D, Berman B, Hazelett D, et al. Granulocyte-Monocyte Progenitors and Monocyte-Dendritic Cell Progenitors Independently Produce Functionally Distinct Monocytes. Immunity. 2017;47:890-902.e4 pubmed publisher
Bowers E, Slaughter A, Frenette P, Kuick R, Pello O, Lucas D. Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nat Med. 2018;24:95-102 pubmed publisher
Sierro F, Evrard M, Rizzetto S, Melino M, Mitchell A, Flórido M, et al. A Liver Capsular Network of Monocyte-Derived Macrophages Restricts Hepatic Dissemination of Intraperitoneal Bacteria by Neutrophil Recruitment. Immunity. 2017;47:374-388.e6 pubmed publisher
Mildner A, Schönheit J, Giladi A, David E, Lara Astiaso D, Lorenzo Vivas E, et al. Genomic Characterization of Murine Monocytes Reveals C/EBP? Transcription Factor Dependence of Ly6C- Cells. Immunity. 2017;46:849-862.e7 pubmed publisher
Takács E, Boto P, Simo E, Csuth T, Toth B, Raveh Amit H, et al. Immunogenic Dendritic Cell Generation from Pluripotent Stem Cells by Ectopic Expression of Runx3. J Immunol. 2017;198:239-248 pubmed
Tan X, Fujiu K, Manabe I, Nishida J, Yamagishi R, Terashima Y, et al. Choroidal Neovascularization Is Inhibited in Splenic-Denervated or Splenectomized Mice with a Concomitant Decrease in Intraocular Macrophage. PLoS ONE. 2016;11:e0160985 pubmed publisher
Ulrich V, Rotllan N, Araldi E, Luciano A, Skroblin P, Abonnenc M, et al. Chronic miR-29 antagonism promotes favorable plaque remodeling in atherosclerotic mice. EMBO Mol Med. 2016;8:643-53 pubmed publisher
Steinmetz O, Hoch S, Avniel Polak S, Gavish K, Eli Berchoer L, Wilensky A, et al. CX3CR1hi Monocyte/Macrophages Support Bacterial Survival and Experimental Infection-Driven Bone Resorption. J Infect Dis. 2016;213:1505-15 pubmed publisher
Foster G, Xu L, Chidambaram A, Soderberg S, Armstrong E, Wu H, et al. CD11c/CD18 Signals Very Late Antigen-4 Activation To Initiate Foamy Monocyte Recruitment during the Onset of Hypercholesterolemia. J Immunol. 2015;195:5380-92 pubmed publisher
Oliveira Lima O, Pinto M, Duchene J, Qadri F, Souza L, Alenina N, et al. Mas receptor deficiency exacerbates lipopolysaccharide-induced cerebral and systemic inflammation in mice. Immunobiology. 2015;220:1311-21 pubmed publisher
Hussein M, Shrestha E, Ouimet M, Barrett T, Leone S, Moore K, et al. LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2. PLoS ONE. 2015;10:e0135218 pubmed publisher
Koyama M, Cheong M, Markey K, Gartlan K, Kuns R, Locke K, et al. Donor colonic CD103+ dendritic cells determine the severity of acute graft-versus-host disease. J Exp Med. 2015;212:1303-21 pubmed publisher
Mairhofer D, Ortner D, Tripp C, Schaffenrath S, Fleming V, Heger L, et al. Impaired gp100-Specific CD8(+) T-Cell Responses in the Presence of Myeloid-Derived Suppressor Cells in a Spontaneous Mouse Melanoma Model. J Invest Dermatol. 2015;135:2785-2793 pubmed publisher
Distel E, Barrett T, Chung K, Girgis N, Parathath S, Essau C, et al. miR33 inhibition overcomes deleterious effects of diabetes mellitus on atherosclerosis plaque regression in mice. Circ Res. 2014;115:759-69 pubmed publisher
Chen S, Feng W, Yang X, Yang W, Ru Y, Liao J, et al. Functional expression of P2X family receptors in macrophages is affected by microenvironment in mouse T cell acute lymphoblastic leukemia. Biochem Biophys Res Commun. 2014;446:1002-9 pubmed publisher
Jackson A, Hegde V, Nagarkatti P, Nagarkatti M. Characterization of endocannabinoid-mediated induction of myeloid-derived suppressor cells involving mast cells and MCP-1. J Leukoc Biol. 2014;95:609-19 pubmed publisher
Johansson J, Pradhan S, Lokteva L, Woodling N, Ko N, Brown H, et al. Suppression of inflammation with conditional deletion of the prostaglandin E2 EP2 receptor in macrophages and brain microglia. J Neurosci. 2013;33:16016-32 pubmed publisher
Ryu J, Davidson B, Xie A, Qi Y, Zha D, Belcik J, et al. Molecular imaging of the paracrine proangiogenic effects of progenitor cell therapy in limb ischemia. Circulation. 2013;127:710-9 pubmed publisher
Smith C, Chang M, Parker K, Beury D, DuHadaway J, Flick H, et al. IDO is a nodal pathogenic driver of lung cancer and metastasis development. Cancer Discov. 2012;2:722-35 pubmed publisher
Jaeger B, Donadieu J, Cognet C, Bernat C, Ordoñez Rueda D, Barlogis V, et al. Neutrophil depletion impairs natural killer cell maturation, function, and homeostasis. J Exp Med. 2012;209:565-80 pubmed publisher
product information
Antigen :
CD115
Apps. Abbrev. :
Flow Cytometry
Cat # :
135505
Clone :
AFS98
Item :
PE anti-mouse CD115 (CSF-1R)
Isotype :
Rat IgG2a, κ
Other Names :
CSF-1R, M-CSFR, c-fms
Size :
50 μg
Price (USD) :
117 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of ligand binding 1 . The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD115 4 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA