product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse CD279 (PD-1)
catalog :
135230
quantity :
100 μg
price :
335 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
29F.1A12
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, mass cytometry
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4b, e5e
Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig 2e
Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
  • flow cytometry; mouse; loading ...
Neumann S, Campbell K, Woodall M, Evans M, Clarkson A, Young S. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s22a
Ho D, Tsui Y, Chan L, Sze K, Zhang X, Cheu J, et al. Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma. Nat Commun. 2021;12:3684 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
  • flow cytometry; mouse; 1:150; loading ...; fig s2l
  • immunohistochemistry; mouse; 1:150; loading ...; fig 5f
Thanabalasuriar A, Chiang A, Morehouse C, Camara M, Hawkins S, Keller A, et al. PD-L1+ neutrophils contribute to injury-induced infection susceptibility. Sci Adv. 2021;7: pubmed publisher
  • flow cytometry; mouse; 1:100; fig 2e
Page N, Lemeille S, Vincenti I, Klimek B, Mariotte A, Wagner I, et al. Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling. Nat Commun. 2021;12:1009 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3i, 3j
Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Tanaka R, Ichimura Y, Kubota N, Saito A, Nakamura Y, Ishitsuka Y, et al. Activation of CD8 T cells accelerates anti-PD-1 antibody-induced psoriasis-like dermatitis through IL-6. Commun Biol. 2020;3:571 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 6d
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a, s3b, s3c, s4a
Cohen G, Chandran P, Lorsung R, Tomlinson L, Sundby M, Burks S, et al. The Impact of Focused Ultrasound in Two Tumor Models: Temporal Alterations in the Natural History on Tumor Microenvironment and Immune Cell Response. Cancers (Basel). 2020;12: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6s1
Canel M, Taggart D, Sims A, Lonergan D, Waizenegger I, Serrels A. T-cell co-stimulation in combination with targeting FAK drives enhanced anti-tumor immunity. elife. 2020;9: pubmed publisher
  • mass cytometry; mouse; 3 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3h
Wirsching H, Zhang H, Szulzewsky F, Arora S, Grandi P, Cimino P, et al. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2b
Grootjans J, Krupka N, Hosomi S, Matute J, Hanley T, Saveljeva S, et al. Epithelial endoplasmic reticulum stress orchestrates a protective IgA response. Science. 2019;363:993-998 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e1b
Karmaus P, Chen X, Lim S, Herrada A, Nguyen T, Xu B, et al. Metabolic heterogeneity underlies reciprocal fates of TH17 cell stemness and plasticity. Nature. 2019;565:101-105 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Shi B, Geng J, Wang Y, Wei H, Walters B, Li W, et al. Foxp1 Negatively Regulates T Follicular Helper Cell Differentiation and Germinal Center Responses by Controlling Cell Migration and CTLA-4. J Immunol. 2018;200:586-594 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Mogilenko D, Shpynov O, Andhey P, Arthur L, Swain A, Esaulova E, et al. Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMK+ CD8+ T Cells as Conserved Hallmark of Inflammaging. Immunity. 2021;54:99-115.e12 pubmed publisher
Sledzińska A, Vila de Mucha M, Bergerhoff K, Hotblack A, Demane D, Ghorani E, et al. Regulatory T Cells Restrain Interleukin-2- and Blimp-1-Dependent Acquisition of Cytotoxic Function by CD4+ T Cells. Immunity. 2020;52:151-166.e6 pubmed publisher
Goldstein J, Valido A, Lewandowski J, Walker R, Mills M, Messemer K, et al. Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Sci Rep. 2019;9:18613 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Nambiar D, Aguilera T, Cao H, Kwok S, Kong C, Bloomstein J, et al. Galectin-1-driven T cell exclusion in the tumor endothelium promotes immunotherapy resistance. J Clin Invest. 2019;129:5553-5567 pubmed publisher
Mintz M, Felce J, Chou M, Mayya V, Xu Y, Shui J, et al. The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity. 2019;: pubmed publisher
Straub T, Pircher H. Enhancing immunity prevents virus-induced T-cell-mediated immunopathology in B cell-deficient mice. Eur J Immunol. 2019;49:782-789 pubmed publisher
Haikala H, Anttila J, Marques E, Raatikainen T, Ilander M, Hakanen H, et al. Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy. Nat Commun. 2019;10:620 pubmed publisher
Bern M, Parikh B, Yang L, Beckman D, Poursine Laurent J, Yokoyama W. Inducible down-regulation of MHC class I results in natural killer cell tolerance. J Exp Med. 2019;216:99-116 pubmed publisher
Tan H, Jegaskanda S, Juno J, Esterbauer R, Wong J, Kelly H, et al. Subdominance and poor intrinsic immunogenicity limit humoral immunity targeting influenza HA stem. J Clin Invest. 2019;129:850-862 pubmed publisher
Mastroianni J, Stickel N, Andrlova H, Hanke K, Melchinger W, Duquesne S, et al. miR-146a Controls Immune Response in the Melanoma Microenvironment. Cancer Res. 2019;79:183-195 pubmed publisher
Wu B, Sun X, Gupta H, Yuan B, Li J, Ge F, et al. Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer. Oncoimmunology. 2018;7:e1500107 pubmed publisher
Preite S, Cannons J, Radtke A, Vujkovic Cvijin I, Gomez Rodriguez J, Volpi S, et al. Hyperactivated PI3Kδ promotes self and commensal reactivity at the expense of optimal humoral immunity. Nat Immunol. 2018;19:986-1000 pubmed publisher
Cheng Y, Zhu X, Wang X, Zhuang Q, Huyan X, Sun X, et al. Trichinella spiralis Infection Mitigates Collagen-Induced Arthritis via Programmed Death 1-Mediated Immunomodulation. Front Immunol. 2018;9:1566 pubmed publisher
Stathopoulou C, Gangaplara A, Mallett G, Flomerfelt F, Liniany L, Knight D, et al. PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells. Immunity. 2018;49:247-263.e7 pubmed publisher
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
Vendetti F, Karukonda P, Clump D, Teo T, Lalonde R, Nugent K, et al. ATR kinase inhibitor AZD6738 potentiates CD8+ T cell-dependent antitumor activity following radiation. J Clin Invest. 2018;128:3926-3940 pubmed publisher
Metzger P, Kirchleitner S, Koenig L, Hörth C, Kobold S, Endres S, et al. Dying cells expose a nuclear antigen cross-reacting with anti-PD-1 monoclonal antibodies. Sci Rep. 2018;8:8810 pubmed publisher
Sun I, Oh M, Zhao L, Patel C, Arwood M, Xu W, et al. mTOR Complex 1 Signaling Regulates the Generation and Function of Central and Effector Foxp3+ Regulatory T Cells. J Immunol. 2018;201:481-492 pubmed publisher
Li R, Liu J, Wu S, Zai X, Li Y, Yang Q, et al. Toll-like receptor 4 signalling regulates antibody response to adenoviral vector-based vaccines by imprinting germinal centre quality. Immunology. 2018;155:251-262 pubmed publisher
Quaranta V, Rainer C, Nielsen S, Raymant M, Ahmed M, Engle D, et al. Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer. Cancer Res. 2018;78:4253-4269 pubmed publisher
Andargachew R, Martinez R, Kolawole E, Evavold B. CD4 T Cell Affinity Diversity Is Equally Maintained during Acute and Chronic Infection. J Immunol. 2018;201:19-30 pubmed publisher
Seydoux E, Liang H, Dubois Cauwelaert N, Archer M, Rintala N, Kramer R, et al. Effective Combination Adjuvants Engage Both TLR and Inflammasome Pathways To Promote Potent Adaptive Immune Responses. J Immunol. 2018;201:98-112 pubmed publisher
Kawano Y, Zavidij O, Park J, Moschetta M, Kokubun K, Mouhieddine T, et al. Blocking IFNAR1 inhibits multiple myeloma-driven Treg expansion and immunosuppression. J Clin Invest. 2018;128:2487-2499 pubmed publisher
Seki A, Rutz S. Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. J Exp Med. 2018;215:985-997 pubmed publisher
Böttcher J, Bonavita E, Chakravarty P, Blees H, Cabeza Cabrerizo M, Sammicheli S, et al. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control. Cell. 2018;172:1022-1037.e14 pubmed publisher
Juchem K, Sacirbegovic F, Zhang C, Sharpe A, RUSSELL K, McNiff J, et al. PD-L1 Prevents the Development of Autoimmune Heart Disease in Graft-versus-Host Disease. J Immunol. 2018;200:834-846 pubmed publisher
Harly C, Cam M, Kaye J, Bhandoola A. Development and differentiation of early innate lymphoid progenitors. J Exp Med. 2018;215:249-262 pubmed publisher
O Neill R, Du W, Mohammadpour H, Alqassim E, Qiu J, Chen G, et al. T Cell-Derived CD70 Delivers an Immune Checkpoint Function in Inflammatory T Cell Responses. J Immunol. 2017;199:3700-3710 pubmed publisher
Roussey J, Viglianti S, Teitz Tennenbaum S, Olszewski M, Osterholzer J. Anti-PD-1 Antibody Treatment Promotes Clearance of Persistent Cryptococcal Lung Infection in Mice. J Immunol. 2017;199:3535-3546 pubmed publisher
Huber M, Suprunenko T, Ashhurst T, Marbach F, Raifer H, Wolff S, et al. IRF9 Prevents CD8+ T Cell Exhaustion in an Extrinsic Manner during Acute Lymphocytic Choriomeningitis Virus Infection. J Virol. 2017;91: pubmed publisher
Goel S, Decristo M, Watt A, BrinJones H, Sceneay J, Li B, et al. CDK4/6 inhibition triggers anti-tumour immunity. Nature. 2017;548:471-475 pubmed publisher
Papa I, Saliba D, Ponzoni M, Bustamante S, Canete P, Gonzalez Figueroa P, et al. TFH-derived dopamine accelerates productive synapses in germinal centres. Nature. 2017;547:318-323 pubmed publisher
Seth P, Csizmadia E, Hedblom A, Vuerich M, Xie H, Li M, et al. Deletion of Lactate Dehydrogenase-A in Myeloid Cells Triggers Antitumor Immunity. Cancer Res. 2017;77:3632-3643 pubmed publisher
Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
Mognol G, Spreafico R, Wong V, Scott Browne J, Togher S, Hoffmann A, et al. Exhaustion-associated regulatory regions in CD8+ tumor-infiltrating T cells. Proc Natl Acad Sci U S A. 2017;114:E2776-E2785 pubmed publisher
Brahmakshatriya V, Kuang Y, Devarajan P, Xia J, Zhang W, Vong A, et al. IL-6 Production by TLR-Activated APC Broadly Enhances Aged Cognate CD4 Helper and B Cell Antibody Responses In Vivo. J Immunol. 2017;198:2819-2833 pubmed publisher
Good Jacobson K, Szumilas C, Chen L, Sharpe A, Tomayko M, Shlomchik M. PD-1 regulates germinal center B cell survival and the formation and affinity of long-lived plasma cells. Nat Immunol. 2010;11:535-42 pubmed publisher
product information
Antigen :
CD279
Apps. Abbrev. :
FC
Cat # :
135230
Clone :
29F.1A12
Item :
Alexa Fluor® 647 anti-mouse CD279 (PD-1)
Isotype :
Rat IgG2a, κ
Other Names :
PD-1, Programmed Death-1, PDCD1
Size :
100 μg
Price (USD) :
335 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
PD-1 cDNA followed by PD-1-Ig fusion protein
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue^3, in vivo blocking of PD-1 binding to its ligands^2,3, and spatial biology (IBEX)^5,6.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA