Ultra-LEAF™ Purified anti-mouse CD117 (c-kit) | BioLegend 135131 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse CD117 (c-kit)

catalog :

135131

quantity :

5 mg

price :

743 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

ACK2

reactivity :

mouse

application :

neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 2a	Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig s2a	Hyrenius Wittsten A, Pilheden M, Sturesson H, Hansson J, Walsh M, Song G, et al. De novo activating mutations drive clonal evolution and enhance clonal fitness in KMT2A-rearranged leukemia. Nat Commun. 2018;9:1770 pubmed publisher
blocking or activating experiments; mouse; fig s2	Palchaudhuri R, Saez B, Hoggatt J, Schajnovitz A, Sykes D, Tate T, et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol. 2016;34:738-45 pubmed publisher
flow cytometry; mouse; loading ...; fig 4a	Kurkewich J, Bikorimana E, Nguyen T, Klopfenstein N, Zhang H, Hallas W, et al. The mirn23a microRNA cluster antagonizes B cell development. J Leukoc Biol. 2016;100:665-677 pubmed
immunohistochemistry - frozen section; mouse; fig 2	Takeyama A, Yoshikawa Y, Ikeo T, Morita S, Hieda Y. Expression patterns of CD66a and CD117 in the mouse submandibular gland. Acta Histochem. 2015;117:76-82 pubmed publisher
	Barkaway A, Rolas L, Joulia R, Bodkin J, Lenn T, Owen Woods C, et al. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage. Immunity. 2021;54:1494-1510.e7 pubmed publisher
	Arensman M, Yang X, Zhong W, Bisulco S, Upeslacis E, Rosfjord E, et al. Anti-tumor immunity influences cancer cell reliance upon ATG7. Oncoimmunology. 2020;9:1800162 pubmed publisher
	Fox A, Dutt T, Karger B, Obregón Henao A, Anderson G, Henao Tamayo M. Acquisition of High-Quality Spectral Flow Cytometry Data. Curr Protoc Cytom. 2020;93:e74 pubmed publisher
	Xie Y, Peng J, Pang J, Guo K, Zhang L, Yin S, et al. Biglycan regulates neuroinflammation by promoting M1 microglial activation in early brain injury after experimental subarachnoid hemorrhage. J Neurochem. 2020;152:368-380 pubmed publisher
	Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
	Agarwal P, Isringhausen S, Li H, Paterson A, He J, Gomariz Á, et al. Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells. Cell Stem Cell. 2019;24:769-784.e6 pubmed publisher

product information

Antigen :

CD117

Apps. Abbrev. :

FC, Block, Depletion

Cat # :

135131

Clone :

ACK2

Item :

Ultra-LEAF™ Purified anti-mouse CD117 (c-kit)

Isotype :

Rat IgG2b, κ

Other Names :

c-KIT, Stem Cell Factor Receptor (SCFR)

Size :

5 mg

Price (USD) :

743 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

Murine IL-3 dependent mast cells

Application Notes :

Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.^3ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;

more info or order :

BioLegend product webpage