product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse Tim-4
catalog :
130008
quantity :
100 μg
price :
347 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
RMT4-54
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 20
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 3c
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig e6e
Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig 3e
Mouhadeb O, Ben Shlomo S, Cohen K, Farkash I, Gruber S, Maharshak N, et al. Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function. Front Immunol. 2018;9:2623 pubmed publisher
  • flow cytometry; mouse; fig 2a
Shaw T, Houston S, Wemyss K, Bridgeman H, Barbera T, Zangerle Murray T, et al. Tissue-resident macrophages in the intestine are long lived and defined by Tim-4 and CD4 expression. J Exp Med. 2018;215:1507-1518 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5c
A Gonzalez N, Quintana J, Garcia Silva S, Mazariegos M, González de la Aleja A, Nicolás Ávila J, et al. Phagocytosis imprints heterogeneity in tissue-resident macrophages. J Exp Med. 2017;214:1281-1296 pubmed publisher
Wu Z, Thierry K, Bachy S, Zhang X, Gamradt P, Hernandez Vargas H, et al. Pericyte stem cells induce Ly6G+ cell accumulation and immunotherapy resistance in pancreatic cancer. EMBO Rep. 2023;24:e56524 pubmed publisher
Slusarczyk P, Mandal P, Zurawska G, Niklewicz M, Chouhan K, Mahadeva R, et al. Impaired iron recycling from erythrocytes is an early hallmark of aging. elife. 2023;12: pubmed publisher
Rosina M, Ceci V, Turchi R, Chuan L, Borcherding N, Sciarretta F, et al. Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue. Cell Metab. 2022;34:533-548.e12 pubmed publisher
Cohen K, Mouhadeb O, Ben Shlomo S, Langer M, Neumann A, Erez N, et al. COMMD10 is critical for Kupffer cell survival and controls Ly6Chi monocyte differentiation and inflammation in the injured liver. Cell Rep. 2021;37:110026 pubmed publisher
Troutman T, Bennett H, Sakai M, Seidman J, Heinz S, Glass C. Purification of mouse hepatic non-parenchymal cells or nuclei for use in ChIP-seq and other next-generation sequencing approaches. STAR Protoc. 2021;2:100363 pubmed publisher
Liu Z, Gu Y, Shin A, Zhang S, Ginhoux F. Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry. STAR Protoc. 2020;1:100029 pubmed publisher
Remmerie A, Martens L, Thoné T, Castoldi A, Seurinck R, Pavie B, et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver. Immunity. 2020;53:641-657.e14 pubmed publisher
Tran S, Baba I, Poupel L, Dussaud S, Moreau M, Gélineau A, et al. Impaired Kupffer Cell Self-Renewal Alters the Liver Response to Lipid Overload during Non-alcoholic Steatohepatitis. Immunity. 2020;53:627-640.e5 pubmed publisher
Liu Z, Gu Y, Chakarov S, Blériot C, Kwok I, Chen X, et al. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019;178:1509-1525.e19 pubmed publisher
De Schepper S, Verheijden S, Aguilera Lizarraga J, Viola M, Boesmans W, Stakenborg N, et al. Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis. Cell. 2018;175:400-415.e13 pubmed publisher
Scott C, T Jonck W, Martens L, Todorov H, Sichien D, Soen B, et al. The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages. Immunity. 2018;49:312-325.e5 pubmed publisher
Baratin M, Simon L, Jorquera A, Ghigo C, Dembele D, Nowak J, et al. T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node. Immunity. 2017;47:349-362.e5 pubmed publisher
Imperatore F, Maurizio J, Vargas Aguilar S, Busch C, Favret J, Kowenz Leutz E, et al. SIRT1 regulates macrophage self-renewal. EMBO J. 2017;36:2353-2372 pubmed publisher
Johnson B, Midura E, Prakash P, Rice T, Kunz N, Kalies K, et al. Neutrophil derived microparticles increase mortality and the counter-inflammatory response in a murine model of sepsis. Biochim Biophys Acta Mol Basis Dis. 2017;1863:2554-2563 pubmed publisher
Yu Y, O Koren E, Hotten D, Kan M, Kopin D, Nelson E, et al. A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues. PLoS ONE. 2016;11:e0150606 pubmed publisher
product information
Antigen :
Tim-4
Apps. Abbrev. :
FC
Cat # :
130008
Clone :
RMT4-54
Item :
Alexa Fluor® 647 anti-mouse Tim-4
Isotype :
Rat IgG2a, κ
Other Names :
T cell immunoglobulin and mucin domain containing protein-4
Size :
100 μg
Price (USD) :
347 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
Mouse TIM4-Ig fusion protein
Application Notes :
Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production^1 and blockade of dendritic cell Tim-4^2.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA